Recombinant Anti-Drosha antibody [EPR23046-123] (ab242147)
![Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] (ab242147)](https://www.abcam.com/ps/products/242/ab242147/Images/ab242147-2-anti-drosha-antibody-epr23046123-immunocytochemistry-nih3t3.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] (ab242147)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23046-123] to Drosha
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Drosha antibody [EPR23046-123]
See all Drosha primary antibodies -
Description
Rabbit monoclonal [EPR23046-123] to Drosha -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, K562, PC-12, RAW 264.7, C6 and NIH/3T3 whole cell lysates. ICC/IF: RAW 264.7 and NIH/3T3 cells. Flow Cyt (intra): RAW 264.7 and NIH/3T3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23046-123 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab242147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
Not suitable for Human or Mouse. |
|
| ICC/IF |
1/50.
Not suitable for Human or Mouse. |
|
| WB |
1/1000. Detects a band of approximately 158 kDa (predicted molecular weight: 159 kDa).
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/500. Not suitable for Human or Mouse. |
|
ICC/IF
1/50. Not suitable for Human or Mouse. |
|
WB
1/1000. Detects a band of approximately 158 kDa (predicted molecular weight: 159 kDa). |
Target
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Function
Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 2 RNase III domains. -
Domain
The 2 RNase III domains form an intramolecular dimer where the domain 1 cuts the 3'strand while the domain 2 cleaves the 5'strand of pri-miRNAs, independently of each other. -
Cellular localization
Nucleus. Nucleus > nucleolus. A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies. - Information by UniProt
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Database links
- Entrez Gene: 29102 Human
- Entrez Gene: 14000 Mouse
- Entrez Gene: 310159 Rat
- Omim: 608828 Human
- SwissProt: Q9NRR4 Human
- SwissProt: Q5HZJ0 Mouse
- Unigene: 97997 Human
- Unigene: 293142 Mouse
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Alternative names
- DROSHA antibody
- Drosha double stranded RNA specific endoribonuclease antibody
- Drosha ribonuclease type III antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] (ab242147)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Drosha with ab242147 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in NIH/3T3 cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Western blot - Anti-Drosha antibody [EPR23046-123] (ab242147)](https://www.abcam.com/ps/products/242/ab242147/Images/ab242147-6-anti-drosha-antibody-epr23046123-western-blot-lysate1.jpg)
Western blot - Anti-Drosha antibody [EPR23046-123] (ab242147)All lanes : Anti-Drosha antibody [EPR23046-123] (ab242147) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lane 2 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 159 kDa
Observed band size: 158 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Weakly reactivity with undetermined proteins in HeLa.
Exposure time: 3 minutes
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![Western blot - Anti-Drosha antibody [EPR23046-123] (ab242147)](https://www.abcam.com/ps/products/242/ab242147/Images/ab242147-5-anti-drosha-antibody-epr23046123-western-blot-lysate.jpg)
Western blot - Anti-Drosha antibody [EPR23046-123] (ab242147)All lanes : Anti-Drosha antibody [EPR23046-123] (ab242147) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 159 kDa
Observed band size: 158 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 48 seconds.
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![Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] (ab242147)](https://www.abcam.com/ps/products/242/ab242147/Images/ab242147-4-anti-drosha-antibody-epr23046123-flow-cytometry-nih3t3.jpg)
Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] (ab242147)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling Drosha with ab242147 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] (ab242147)](https://www.abcam.com/ps/products/242/ab242147/Images/ab242147-1-anti-drosha-antibody-epr23046123-immunocytochemistry-raw2647.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] (ab242147)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Raw264.7 cells labelling Drosha with ab242147 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in Raw264.7 cell line. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] (ab242147)](https://www.abcam.com/ps/products/242/ab242147/Images/ab242147-3-anti-drosha-antibody-epr23046123-flow-cytometry-raw-2647.jpg)
Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] (ab242147)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Raw 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Drosha with ab242147 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Anti-Drosha antibody [EPR23046-123] (ab242147)](https://www.abcam.com/ps/products/242/ab242147/Images/ab242147-7-benefits-of-recombinant-antibodies.png)
Anti-Drosha antibody [EPR23046-123] (ab242147)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab242147 has not yet been referenced specifically in any publications.