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RecombinantRabMAb

Recombinant Anti-Drosha antibody [EPR23046-123] (ab242147)

Submit a review Submit a question References (1)
Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] (ab242147)
  • Western blot - Anti-Drosha antibody [EPR23046-123] (ab242147)
  • Western blot - Anti-Drosha antibody [EPR23046-123] (ab242147)
  • Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] (ab242147)
  • Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] (ab242147)
  • Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] (ab242147)
  • Anti-Drosha antibody [EPR23046-123] (ab242147)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23046-123] to Drosha
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Drosha antibody [EPR23046-123]
    See all Drosha primary antibodies

  • Description

    Rabbit monoclonal [EPR23046-123] to Drosha

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, K562, PC-12, RAW 264.7, C6 and NIH/3T3 whole cell lysates. ICC/IF: RAW 264.7 and NIH/3T3 cells. Flow Cyt (intra): RAW 264.7 and NIH/3T3 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab242147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/500.

Not suitable for Human or Mouse.
ICC/IF
1/50.

Not suitable for Human or Mouse.
WB
1/1000. Detects a band of approximately 158 kDa (predicted molecular weight: 159 kDa).
Notes
Flow Cyt (Intra)
1/500.

Not suitable for Human or Mouse.
ICC/IF
1/50.

Not suitable for Human or Mouse.
WB
1/1000. Detects a band of approximately 158 kDa (predicted molecular weight: 159 kDa).
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Contains 1 DRBM (double-stranded RNA-binding) domain.
    Contains 2 RNase III domains.

  • Domain

    The 2 RNase III domains form an intramolecular dimer where the domain 1 cuts the 3'strand while the domain 2 cleaves the 5'strand of pri-miRNAs, independently of each other.

  • Cellular localization

    Nucleus. Nucleus > nucleolus. A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies.
  • Information by UniProt

  • Database links

    • Alternative names

      • DROSHA antibody
      • Drosha double stranded RNA specific endoribonuclease antibody
      • Drosha ribonuclease type III antibody
      • Etohi2 antibody
      • HSA242976 antibody
      • Nuclear RNase III Drosha antibody
      • p241 antibody
      • Protein Drosha antibody
      • Putative protein p241 which interacts with transcription factor Sp1 antibody
      • Putative ribonuclease III antibody
      • RANSE3L antibody
      • Ribonuclease 3 antibody
      • Ribonuclease III antibody
      • Ribonuclease III nuclear antibody
      • Ribonuclease type III nuclear antibody
      • RibonucleaseIII antibody
      • RN 3 antibody
      • RN3 antibody
      • RNase 3 antibody
      • RNase III antibody
      • RNase3 antibody
      • RNASE3L antibody
      • RNaseIII antibody
      • RNASEN antibody
      • RNC_HUMAN antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] (ab242147)
      Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] (ab242147)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Drosha with ab242147 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in NIH/3T3 cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Western blot - Anti-Drosha antibody [EPR23046-123] (ab242147)
      Western blot - Anti-Drosha antibody [EPR23046-123] (ab242147)
      All lanes : Anti-Drosha antibody [EPR23046-123] (ab242147) at 1/1000 dilution

      Lane 1 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
      Lane 2 : C6 (rat glial tumor glial cell), whole cell lysate
      Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 159 kDa
      Observed band size: 158 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Weakly reactivity with undetermined proteins in HeLa.

      Exposure time: 3 minutes

    • Western blot - Anti-Drosha antibody [EPR23046-123] (ab242147)
      Western blot - Anti-Drosha antibody [EPR23046-123] (ab242147)
      All lanes : Anti-Drosha antibody [EPR23046-123] (ab242147) at 1/1000 dilution

      Lane 1 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
      Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
      Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 159 kDa
      Observed band size: 158 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Exposure time: 48 seconds.

    • Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] (ab242147)
      Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] (ab242147)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling Drosha with ab242147 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] (ab242147)
      Immunocytochemistry/ Immunofluorescence - Anti-Drosha antibody [EPR23046-123] (ab242147)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Raw264.7 cells labelling Drosha with ab242147 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in Raw264.7 cell line. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] (ab242147)
      Flow Cytometry (Intracellular) - Anti-Drosha antibody [EPR23046-123] (ab242147)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Raw 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Drosha with ab242147 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Anti-Drosha antibody [EPR23046-123] (ab242147)
      Anti-Drosha antibody [EPR23046-123] (ab242147)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab242147? Please let us know so that we can cite the reference in this datasheet.

    ab242147 has been referenced in 1 publication.

    • Peng YH  et al. Micro ribonucleic acid-363 regulates the phosphatidylinositol 3-kinase/threonine protein kinase axis by targeting NOTCH1 and forkhead box C2, leading to hepatic glucose and lipids metabolism disorder in type 2 diabetes mellitus. J Diabetes Investig 13:236-248 (2022). PubMed: 34739190

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