Name: Anti-DRP1 antibody [EPR19274]
Brand: RabMAb
SKU: ab184247
Rating: 5 (4 reviews)

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Western blot - Anti-DRP1 antibody [EPR19274] (ab184247)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19274] to DRP1
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-P
Reacts with: Mouse, Rat, Human

APC Carrier Free PE

Product name

Anti-DRP1 antibody [EPR19274]
See all DRP1 primary antibodies
Description

Rabbit monoclonal [EPR19274] to DRP1
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal kidney, rat brain, rat heart and mouse brain lysates; A549, U-2 OS, HeLa, Jurkat, HEK-293, HCT 116, PC-12 and NIH/3T3 whole cell lysates. IHC-P: Mouse cerebrum and rat cerebellum tissues. ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt (intra): NIH/3T3 cells. IP: HeLa whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19274
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human DRP1 protein (ab153041)
Related Products
- VeriBlot for IP Detection Reagent (HRP) (ab131366)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/70.
WB	(4)	1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
ICC/IF		1/250.
IP		1/30.
IHC-P		1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC is recommended for rat and mouse only.

Notes
Flow Cyt (Intra) 1/70.
WB 1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
ICC/IF 1/250.
IP 1/30.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC is recommended for rat and mouse only.

Function

Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport.
Isoform 1 and isoform 4 inhibit peroxisomal division when overexpressed.
Tissue specificity

Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons.
Involvement in disease

Note=May be associated with Alzheimer disease through beta-amyloid-induced increased S-nitrosylation of DNM1L, which triggers, directly or indirectly, excessive mitochondrial fission, synaptic loss and neuronal damage.
Sequence similarities

Belongs to the dynamin family.
Contains 1 GED domain.
Domain

The GED domain folds back to interact, in cis, with the GTP-binding domain and middle domain, and interacts, in trans, with the GED domains of other DNM1L molecules, and is thus critical for activating GTPase activity and for DNM1L dimerization.
Post-translational
modifications

Phosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-637 inhibits mitochondrial fissin probably through preventing intramolecular interaction. Dephosphorylated on this site by PPP3CA which promotes mitochondrial fission. Phosphorylation on Ser-616 also promotes mitochondrial fission.
Sumoylated on various lysine residues within the B domain. Desumoylated by SENP5 during G2/M transition of mitosis. Appears to be linked to its catalytic activity.
S-nitrosylation increases DNM1L dimerization, mitochondrial fission and causes neuronal damage.
Ubiquitination by MARCH5 affects mitochondrial morphology.
Cellular localization

Cytoplasm > cytosol. Golgi apparatus. Endomembrane system. Mainly cytosolic. Translocated to the mitochondrial membrane through interaction with FIS1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear. In some cell types, localizes to the Golgi complex.
Target information above from: UniProt accession O00429 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 10059 Human
- Entrez Gene: 74006 Mouse
- Entrez Gene: 114114 Rat
- Omim: 603850 Human
- SwissProt: O00429 Human
- SwissProt: Q8K1M6 Mouse
- SwissProt: O35303 Rat
- Unigene: 556296 Human
- Unigene: 218820 Mouse
- Unigene: 216851 Rat
see all
Alternative names
- DLP1 antibody
- dnm1l antibody
- DNM1L_HUMAN antibody
- Dnm1p/Vps1p-like protein antibody
- dnml1 antibody
- DRP1 antibody
- DVLP antibody
- Dymple antibody
- Dynamin 1 like antibody
- Dynamin family member proline-rich carboxyl-terminal domain less antibody
- Dynamin like protein antibody
- Dynamin related protein 1 antibody
- Dynamin-1-like protein antibody
- Dynamin-like protein 4 antibody
- Dynamin-like protein antibody
- Dynamin-like protein IV antibody
- Dynamin-related protein 1 antibody
- DYNIV 11 antibody
- EMPF antibody
- EMPF1 antibody
- FLJ41912 antibody
- HdynIV antibody
- VPS1 antibody
see all

Western blot - Anti-DRP1 antibody [EPR19274] (ab184247)

All lanes : Anti-DRP1 antibody [EPR19274] (ab184247) at 1/1000 dilution

Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 6 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 83 kDa
Observed band size: 83 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1 and 2: 3 minutes; Lane 3: 30 seconds; Lane 4,5 and 6: 8 seconds.
DRP1 can be SUMOylated, as described in the literature (PMID: 19638400).
Immunocytochemistry/ Immunofluorescence - Anti-DRP1 antibody [EPR19274] (ab184247)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling DRP1 with ab184247 at 1/250 dilution, followed by Goat anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasm staining on HeLa cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [EPR19274] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab184247 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-DRP1 antibody [EPR19274] (ab184247)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling DRP1 with ab184247 at 1/70 dilution (red) compared with a Rabbit IgG,monoclonal -Isotype Control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
Western blot - Anti-DRP1 antibody [EPR19274] (ab184247)

Anti-DRP1 antibody [EPR19274] (ab184247) at 1/1000 dilution + Human fetal kidney lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

Predicted band size: 83 kDa
Observed band size: 83 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-DRP1 antibody [EPR19274] (ab184247)

All lanes : Anti-DRP1 antibody [EPR19274] (ab184247) at 1/1000 dilution

Lane 1 : Rat brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Mouse brain lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 83 kDa
Observed band size: 83 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Lane 1: 2 seconds; Lane 2: 8 seconds; Lane 3: 3 seconds.
Western blot - Anti-DRP1 antibody [EPR19274] (ab184247)

All lanes : Anti-DRP1 antibody [EPR19274] (ab184247) at 1/1000 dilution

Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 83 kDa
Observed band size: 83 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19274] (ab184247)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling DRP1 with ab184247 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse cerebrum is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19274] (ab184247)

Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling DRP1 with ab184247 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on rat cerebellum is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-DRP1 antibody [EPR19274] (ab184247)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling DRP1 with ab184247 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasm staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [EPR19274] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab184247 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
Immunoprecipitation - Anti-DRP1 antibody [EPR19274] (ab184247)

DRP1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab184247 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184247 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate 10µg (Input).
Lane 2: ab184247 IP in HeLa whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR19274]-Isotype Control (ab172730) instead of ab184247 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.

Note: DRP1 can be SUMOylated, as described in the literature (PMID: 19638400).
Anti-DRP1 antibody [EPR19274] (ab184247)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

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Publishing research using ab184247? Please let us know so that we can cite the reference in this datasheet.

ab184247 has been referenced in 83 publications.

Xu W et al. Melanocortin 1 receptor attenuates early brain injury following subarachnoid hemorrhage by controlling mitochondrial metabolism via AMPK/SIRT1/PGC-1a pathway in rats. Theranostics 11:522-539 (2021). PubMed: 33391490
Fan H et al. Heat shock protein 22 modulates NRF1/TFAM-dependent mitochondrial biogenesis and DRP1-sparked mitochondrial apoptosis through AMPK-PGC1a signaling pathway to alleviate the early brain injury of subarachnoid hemorrhage in rats. Redox Biol 40:101856 (2021). PubMed: 33472123
Zhang XL et al. Anti-inflammatory and neuroprotective effects of natural cordycepin in rotenone-induced PD models through inhibiting Drp1-mediated mitochondrial fission. Neurotoxicology 84:1-13 (2021). PubMed: 33549657
Zhou M et al. Acupoint catgut embedding improves senescence in a rat model of ageing by regulating mitophagy via the PINK1 pathway. J Cell Mol Med 25:3816-3828 (2021). PubMed: 33645011
Feng L et al. Hepatic Knockdown of Endothelin Type A Receptor (ETAR) Ameliorates Hepatic Insulin Resistance and Hyperglycemia Through Suppressing p66Shc-Mediated Mitochondrial Fragmentation in High-Fat Diet-Fed Mice. Diabetes Metab Syndr Obes 14:963-981 (2021). PubMed: 33688230

View all Publications for this product

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1-4 of 4 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (Dopaminergic Neurons)

Gel Running Conditions

Reduced Denaturing (NuPage 4-12% MES buffer)

Loading amount

30 µg

Specification

Dopaminergic Neurons

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 22 2020

Application

Western blot

Sample

Drosophila melanogaster Tissue lysate - whole (DM head tissue lysate)

Gel Running Conditions

Reduced Denaturing (4-15%)

Loading amount

72 µg

Specification

DM head tissue lysate

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 11 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (Multiple myeloma cancer cell line lysates)

Gel Running Conditions

Reduced Denaturing (10)

Loading amount

20 µg

Specification

Multiple myeloma cancer cell line lysates

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 27 2019

Application

Western blot

Sample

Human Cell lysate - whole cell (Leukemic Cancer Cell lines)

Gel Running Conditions

Reduced Denaturing (10)

Loading amount

20 µg

Specification

Leukemic Cancer Cell lines

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 13 2017

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Domain

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (83)

Customer reviews and Q&As

Western blot abreview for Anti-DRP1 antibody [EPR19274]

Western blot abreview for Anti-DRP1 antibody [EPR19274]

Western blot abreview for Anti-DRP1 antibody [EPR19274]

Western blot abreview for Anti-DRP1 antibody [EPR19274]

Post-translational
modifications