Recombinant Anti-DTN-A antibody [EPR14112] (ab191395)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14112] to DTN-A
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DTN-A antibody [EPR14112]
See all DTN-A primary antibodies -
Description
Rabbit monoclonal [EPR14112] to DTN-A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain, Human cerebellum and skeletal muscle lysats; A673 and U87-MG cell lysates; Human thyroid carcinoma tissue; U87-MG and A673 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14112 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/250.
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Flow Cyt (Intra) |
1/130.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/250. |
Flow Cyt (Intra)
1/130. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
May be involved in the formation and stability of synapses as well as being involved in the clustering of nicotinic acetylcholine receptors. -
Tissue specificity
Highly expressed in brain, skeletal and cardiac muscles, and expressed at lower levels in lung, liver and pancreas. Isoform 2 is not expressed in cardiac muscle. Isoform 7 and isoform 8 are only expressed in muscle. -
Involvement in disease
Defects in DTNA are the cause of left ventricular non-compaction type 1 (LVNC1) [MIM:604169]. Left ventricular non-compaction is due to an arrest of myocardial morphogenesis. The disorder is characterized by a hypertrophic left ventricule with deep trabeculations and with poor systolic function, with or without associated left ventricular dilation. In some cases, it is associated with other congenital heart anomalies such as ventricular septal defects, pulmonic stenosis and atrial septal defects. The right ventricle may also be affected. -
Sequence similarities
Belongs to the dystrophin family. Dystrobrevin subfamily.
Contains 1 ZZ-type zinc finger. -
Domain
The coiled coil domain mediates the interaction with dystrophin and utrophin. -
Post-translational
modificationsPhosphorylation of DTN-1 on tyrosine kinase substrate domain present in the C-terminus. -
Cellular localization
Cytoplasm. Cell junction > synapse. Cell membrane. In peripheral nerves, co-localizes with MAGEE1 in the Schwann cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1837 Human
- Entrez Gene: 13527 Mouse
- Entrez Gene: 307548 Rat
- Omim: 601239 Human
- SwissProt: Q9Y4J8 Human
- SwissProt: Q9D2N4 Mouse
- Unigene: 643454 Human
- Unigene: 94371 Mouse
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Alternative names
- Alpha-dystrobrevin antibody
- D18S892E antibody
- DRP3 antibody
see all
Images
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All lanes : Anti-DTN-A antibody [EPR14112] (ab191395) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human cerebellum lysate
Lane 3 : Human skeletal muscle lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 84 kDa -
Immunofluorescent analysis of U87-MG cells (4% formaldehyde-fixed; 0.1% Triton X-100 permeabilized) labeling DTN-A with ab191395 at 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at 1/200 dilution. Counter-stained with DAPI (blue). Negative controls: Primary ab concentration (anti-DTNA) is 1/250 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.
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All lanes : Anti-DTN-A antibody [EPR14112] (ab191395) at 1/10000 dilution
Lane 1 : A673 cell lysate
Lane 2 : U87-MG cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 84 kDa -
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling DTN-A with ab191395 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Inset: Negative control: using PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of A673 cells (paraformaldehyde-fixed, 2%)labeling DTN-A with ab191395 at 1/130 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab191395 has not yet been referenced specifically in any publications.