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RecombinantRabMAb

Recombinant Anti-DUSP3 antibody [EPR5492] - BSA and Azide free (ab248113)

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Western blot - Anti-DUSP3 antibody [EPR5492] - BSA and Azide free (ab248113)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP3 antibody [EPR5492] - BSA and Azide free (ab248113)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP3 antibody [EPR5492] - BSA and Azide free (ab248113)
  • Anti-DUSP3 antibody [EPR5492] - BSA and Azide free (ab248113)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5492] to DUSP3 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-DUSP3 antibody [EPR5492] - BSA and Azide free
    See all DUSP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR5492] to DUSP3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248113 is the carrier-free version of ab125077.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).

Target

  • Function

    Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2.

  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 tyrosine-protein phosphatase domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Dual specificity phosphatase 3 antibody
    • Dual specificity protein phosphatase 3 antibody
    • Dual specificity protein phosphatase VHR antibody
    • DUS3_HUMAN antibody
    • DUSP3 antibody
    • Serine/threonine specific protein phosphatase antibody
    • Vaccinia H1-related phosphatase antibody
    • Vaccinia virus phosphatase VH1 related antibody
    • VHR antibody
    see all

Images

  • Western blot - Anti-DUSP3 antibody [EPR5492] - BSA and Azide free (ab248113)
    Western blot - Anti-DUSP3 antibody [EPR5492] - BSA and Azide free (ab248113)
    All lanes : Anti-DUSP3 antibody [EPR5492] (ab125077) at 1/50000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : MCF-7 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 20 kDa



    This data was developed using ab125077, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP3 antibody [EPR5492] - BSA and Azide free (ab248113)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP3 antibody [EPR5492] - BSA and Azide free (ab248113)
    This data was developed using ab125077, the same antibody clone in a different buffer formulation.ab125077, at 1/100, staining DUSP3 in paraffin-embedded Human ovarian cancer tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP3 antibody [EPR5492] - BSA and Azide free (ab248113)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP3 antibody [EPR5492] - BSA and Azide free (ab248113)
    This data was developed using ab125077, the same antibody clone in a different buffer formulation.ab125077, at 1/100, staining DUSP3 in paraffin-embedded Mouse kidney tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-DUSP3 antibody [EPR5492] - BSA and Azide free (ab248113)
    Anti-DUSP3 antibody [EPR5492] - BSA and Azide free (ab248113)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab248113? Please let us know so that we can cite the reference in this datasheet.

ab248113 has not yet been referenced specifically in any publications.

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