Recombinant Anti-DUSP3 antibody [EPR5492] - BSA and Azide free (ab248113)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5492] to DUSP3 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-DUSP3 antibody [EPR5492] - BSA and Azide free
See all DUSP3 primary antibodies -
Description
Rabbit monoclonal [EPR5492] to DUSP3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab248113 is the carrier-free version of ab125077.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5492 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
|
Notes |
---|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). |
Target
-
Function
Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 1845 Human
- Entrez Gene: 72349 Mouse
- Entrez Gene: 498003 Rat
- GenBank: NP_004081.1 Human
- Omim: 600183 Human
- SwissProt: P51452 Human
- SwissProt: Q9D7X3 Mouse
- Unigene: 181046 Human
see all -
Alternative names
- Dual specificity phosphatase 3 antibody
- Dual specificity protein phosphatase 3 antibody
- Dual specificity protein phosphatase VHR antibody
see all
Images
-
All lanes : Anti-DUSP3 antibody [EPR5492] (ab125077) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 20 kDaThis data was developed using ab125077, the same antibody clone in a different buffer formulation.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP3 antibody [EPR5492] - BSA and Azide free (ab248113)This data was developed using ab125077, the same antibody clone in a different buffer formulation.ab125077, at 1/100, staining DUSP3 in paraffin-embedded Human ovarian cancer tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUSP3 antibody [EPR5492] - BSA and Azide free (ab248113)This data was developed using ab125077, the same antibody clone in a different buffer formulation.ab125077, at 1/100, staining DUSP3 in paraffin-embedded Mouse kidney tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab248113 has not yet been referenced specifically in any publications.