Anti-E Cadherin antibody - Intercellular Junction Marker (ab15148)

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.
I would like to reassure you that ab15148 is tested and covered by our 6 month guarantee for use in WB and human samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.
Reviewing this case, I would like to offer some suggestions to help optimise the results from ab15148.
1.) I recommend to run a no primary control, to make sure that it is not the secondary antibody producing the extra bands at 50 and 90kDa.
2.) When testing our antibodies, our lab uses 5% BSA as a blocking reagent, so I recommend switching to this instead of milk. Some antibodies give stronger, more specific signals on blots blocked with BSA instead of milk, so doing this may improve the results you are seeing, and reduce the non-specific bands. An example of the above is the western blot obtained with the Abcam GAPDH antibody ab9385 : www.abcam.com/ab9385
3.) Since the smallest extra bands are only in the positive control and it is smaller than the expected band, this could be a degradation band. I therefore suggest to review the proteinase inhibitors and always freshly prepare them.
I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

Thank you for contacting us.
The immunogen of ab53033 corresponds to the internal sequence of E-cadherin so this may not suitable as per your requirements.
The immunogens of antibodies ab15148, ab77287, ab128804, and ab133597 are located within the extra cellular domain of E-cadherin so these antibodies might be suitable. ab133597 has been tested in ICC/IF with mouse and rat cells so it is the most suitable candidate. However the other antibodies can be offered with either mouse or rat or with ICC/IF 100% Abreview discount.
The Abpromise guarantee is with ICC/IF when performed on fixed cells we do not know which antibody would be suitable for live cell imaging so the suitability has t o be determined by the end user.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Thank you for your phone call. Our current E Cadherin antibodies that bind to an extracellular domain of the human protein are:

ab8050
ab14015
ab15148
ab15150
ab22585
ab40772
ab45990
ab77287

I hope this helps, please let me know if you would like me to narrow down the list by any additional criteria.

Thank you for confirming this information and for your help and cooperation with this case. As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order. As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. The reference number, or credit ID, is xxxx. Please refer to this number in any correspondence with the accounting department. I would like to wish the customer good luck with their research. The technical team is always at your service, should you require further expert advice.