Anti-E. coli antibody (ab137967)
Key features and details
- Rabbit polyclonal to E. coli
- Suitable for: ELISA, ICC/IF
- Reacts with: Escherichia coli
- Isotype: IgG
Overview
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Product name
Anti-E. coli antibody
See all E. coli primary antibodies -
Description
Rabbit polyclonal to E. coli -
Host species
Rabbit -
Specificity
This antibody is specific for many “O” and “K” antigenic serotypes of Escherichia coli. Antiserum is not absorbed and does cross-react with related Enterobacteriaceae. ab137967 will remove E. coli proteins from recombinant preparations. -
Tested applications
Suitable for: ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Tissue, cells or virus corresponding to E. coli. Tissue/ cell preparation (Mixture of E. coli serotypes: K12, O111, O55, O125, O20, and O157).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Protein A chromatography. >95% pure. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration. PubMed: 28662099
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| Notes |
|---|
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 28662099 |
Target
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Relevance
Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins. -
Alternative names
- Escherichia coli antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-E. coli antibody (ab137967)Murphy et al. PLoS One. 2017 Jun 29;12(6):e0180034. doi: 10.1371/journal.pone.0180034. eCollection 2017. Figure 3 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Balb/C mice bearing s.c. CT26 flank tumours stained for E. coli (green) using ab137967 in IF. A donkey anti-rabbit Alexa Fluor 488-conjugated anti-Ig was used as the secondary antibody. Blue: DAPI.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab137967 has been referenced in 19 publications.
- Moteshareie H et al. Rapid, Sensitive, and Selective Quantification of Bacillus cereus Spores Using xMAP Technology. Microorganisms 10:N/A (2022). PubMed: 35889128
- Hou Z et al. Intestinal epithelial β Klotho is a critical protective factor in alcohol-induced intestinal barrier dysfunction and liver injury. EBioMedicine 82:104181 (2022). PubMed: 35908416
- Yang X et al. Molecular mechanisms of the antibacterial activity of polyimide fibers in a skin-wound model with Gram-positive and Gram-negative bacterial infection in vivo. Nanoscale Adv 4:3043-3053 (2022). PubMed: 36133513
- Singh KS et al. IspH inhibitors kill Gram-negative bacteria and mobilize immune clearance. Nature 589:597-602 (2021). PubMed: 33361818
- Nickerson KP et al. A Versatile Human Intestinal Organoid-Derived Epithelial Monolayer Model for the Study of Enteric Pathogens. Microbiol Spectr 9:e0000321 (2021). PubMed: 34106568