Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab264182)
Key features and details
- Rabbit polyclonal to E1 Ubiquitin Activating Enzyme 1/UBA1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody
See all E1 Ubiquitin Activating Enzyme 1/UBA1 primary antibodies -
Description
Rabbit polyclonal to E1 Ubiquitin Activating Enzyme 1/UBA1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow
-
Immunogen
Synthetic peptide aa 1008-1058. The exact sequence is proprietary.
Database link: P22314 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates; IHC-P: Human prostate carcinoma tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100 - 1/500.
|
|
| WB |
1/2000 - 1/10000.
|
| Notes |
|---|
|
IHC-P
1/100 - 1/500. |
|
WB
1/2000 - 1/10000. |
Target
-
Function
Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. -
Pathway
Protein modification; protein ubiquitination. -
Involvement in disease
Defects in UBA1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2) [MIM:301830]; also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures. -
Sequence similarities
Belongs to the ubiquitin-activating E1 family. -
Post-translational
modificationsISGylated. - Information by UniProt
-
Database links
- Entrez Gene: 7317 Human
- Entrez Gene: 22201 Mouse
- Entrez Gene: 314432 Rat
- Omim: 314370 Human
- SwissProt: P22314 Human
- SwissProt: Q02053 Mouse
- SwissProt: Q5U300 Rat
- Unigene: 533273 Human
see all -
Alternative names
- A1S9 antibody
- A1S9 protein antibody
- A1S9T and BN75 temperature sensitivity complementing antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab264182)
Immunohistochemical analysis of formalin/PFA-fixed, paraffin-embedded human prostate carcinoma tissue staining E1 Ubiquitin Activating Enzyme 1/UBA1 with ab264182 at 1/200 dilution. Detection: DAB.
-
Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab264182)All lanes : Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab264182) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Lane 5 : NIH/3T3 whole cell lysate at 50 µg
Exposure time: 10 seconds4-8% SDS-PAGE.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab264182 has not yet been referenced specifically in any publications.