Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab34711)
Key features and details
- Rabbit polyclonal to E1 Ubiquitin Activating Enzyme 1/UBA1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody
See all E1 Ubiquitin Activating Enzyme 1/UBA1 primary antibodies -
Description
Rabbit polyclonal to E1 Ubiquitin Activating Enzyme 1/UBA1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Dog
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Immunogen
Fusion protein corresponding to Human E1 Ubiquitin Activating Enzyme 1/UBA1.
Database link: P22314 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
The product was purified from monospecific antiserum by a multi step procedure. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
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| IHC-P |
Use a concentration of 2 - 20 µg/ml.
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| Notes |
|---|
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WB
1/1000 - 1/5000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa). |
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IHC-P
Use a concentration of 2 - 20 µg/ml. |
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ICC/IF
Use a concentration of 5 µg/ml. |
Target
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Function
Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. -
Pathway
Protein modification; protein ubiquitination. -
Involvement in disease
Defects in UBA1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2) [MIM:301830]; also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures. -
Sequence similarities
Belongs to the ubiquitin-activating E1 family. -
Post-translational
modificationsISGylated. - Information by UniProt
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Database links
- Entrez Gene: 7317 Human
- Entrez Gene: 22201 Mouse
- Omim: 314370 Human
- SwissProt: P22314 Human
- SwissProt: Q02053 Mouse
- Unigene: 533273 Human
- Unigene: 1104 Mouse
- Unigene: 474674 Mouse
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Alternative names
- A1S9 antibody
- A1S9 protein antibody
- A1S9T and BN75 temperature sensitivity complementing antibody
see all
Images
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Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab34711)All lanes : Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab34711) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg/ml per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 118 kDa
Observed band size: 118 kDa
Additional bands at: 35 kDa, 53 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab34711)This image shows human lung tissue stained with ab34711 at 10µg/ml. In many cells apunctate nuclear staining was observed. Other cells showed both cytoplasmic and nuclear staining.
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Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab34711)ICC/IF image of ab34711 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34711, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab34711)ab34711 (4µg/ml) staining E1 Ubiquitin activating enzyme in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is nuclear and weak cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab34711 has been referenced in 8 publications.
- Chen Z et al. Spermidine activates RIP1 deubiquitination to inhibit TNF-a-induced NF-?B/p65 signaling pathway in osteoarthritis. Cell Death Dis 11:503 (2020). PubMed: 32632306
- Shruthi K et al. Ubiquitin-proteasome system and ER stress in the brain of diabetic rats. J Cell Biochem 120:5962-5973 (2019). PubMed: 30317658
- Reddy SS et al. 4-PBA prevents diabetic muscle atrophy in rats by modulating ER stress response and ubiquitin-proteasome system. Chem Biol Interact 306:70-77 (2019). PubMed: 30980806
- Powis RA et al. Systemic restoration of UBA1 ameliorates disease in spinal muscular atrophy. JCI Insight 1:e87908 (2016). WB . PubMed: 27699224
- Park JH et al. PUGNAc induces protein ubiquitination in C2C12 myotube cells. Cell Biochem Funct 33:525-33 (2015). PubMed: 26531776
- Estrada-Bernal A et al. Functional complexity of the axonal growth cone: a proteomic analysis. PLoS One 7:e31858 (2012). WB, ICC/IF ; Rat . PubMed: 22384089
- Hjerpe R et al. Changes in the ratio of free NEDD8 to ubiquitin triggers NEDDylation by ubiquitin enzymes. Biochem J 441:927-36 (2012). WB . PubMed: 22004789
- Guinez C et al. Protein ubiquitination is modulated by O-GlcNAc glycosylation. FASEB J 22:2901-11 (2008). WB, IP ; Human . PubMed: 18434435
