Recombinant Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)
![Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)](https://www.abcam.com/ps/products/250/ab250138/Images/ab250138-408326-anti-e1-ubiquitin-activating-enzyme-1-uba1-antibody-epr14203-b-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14203(B)] to E1 Ubiquitin Activating Enzyme 1/UBA1 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free
See all E1 Ubiquitin Activating Enzyme 1/UBA1 primary antibodies -
Description
Rabbit monoclonal [EPR14203(B)] to E1 Ubiquitin Activating Enzyme 1/UBA1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250138 is the carrier-free version of ab180125.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14203(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| ICC/IF |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 117 kDa (predicted molecular weight: 118 kDa).
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| Notes |
|---|
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 117 kDa (predicted molecular weight: 118 kDa). |
Target
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Function
Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. -
Pathway
Protein modification; protein ubiquitination. -
Involvement in disease
Defects in UBA1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2) [MIM:301830]; also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures. -
Sequence similarities
Belongs to the ubiquitin-activating E1 family. -
Post-translational
modificationsISGylated. - Information by UniProt
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Database links
- Entrez Gene: 7317 Human
- Entrez Gene: 22201 Mouse
- Entrez Gene: 314432 Rat
- Omim: 314370 Human
- SwissProt: P22314 Human
- SwissProt: Q02053 Mouse
- SwissProt: Q5U300 Rat
- Unigene: 533273 Human
see all -
Alternative names
- A1S9 antibody
- A1S9 protein antibody
- A1S9T and BN75 temperature sensitivity complementing antibody
see all
Images
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Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)All lanes : Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125) at 1/5000 dilution
Lane 1 : Fetal brain tissue lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 118 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?This data was developed using ab180125, the same antibody clone in a different buffer formulation.
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![Flow Cytometry (Intracellular) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)](https://www.abcam.com/ps/products/250/ab250138/Images/ab250138-3-anti-e1-ubiquitin-activating-enzyme-1-uba1-antibody-epr14203-b-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)This data was developed using ab180125, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/70 dilution (red) compared to a rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/75 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)](https://www.abcam.com/ps/products/250/ab250138/Images/ab250138-2-anti-e1-ubiquitin-activating-enzyme-1-uba1-antibody-epr14203-b-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)This data was developed using ab180125, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of acetone-fixed Jurkat cells labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/100 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/250 dilution. Counter stained with DAPI (blue). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)](https://www.abcam.com/ps/products/250/ab250138/Images/ab250138-1-anti-e1-ubiquitin-activating-enzyme-1-uba1-antibody-epr14203-b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)This data was developed using ab180125, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)](https://www.abcam.com/ps/products/250/ab250138/Images/ab250138-5-benefits-of-recombinant-antibodies.png)
Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250138 has not yet been referenced specifically in any publications.
