Recombinant Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)
![Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)](https://www.abcam.com/ps/products/181/ab181225/Images/ab181225-484492-AntiE1-Ubiquitin-Activating-Enzyme-1UBA1-antibody-EPR14204B-western-blot-human-recombinant-protein.png "Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14204(B)] to E1 Ubiquitin Activating Enzyme 1/UBA1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)]
See all E1 Ubiquitin Activating Enzyme 1/UBA1 primary antibodies -
Description
Rabbit monoclonal [EPR14204(B)] to E1 Ubiquitin Activating Enzyme 1/UBA1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain, K562, HeLa and Jurkat lysates; Human kidney tissue; HeLa cells; K562 cells., GST-tagged full-length human recombinant UBA7 and UBA1.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14204(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/10000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| ICC/IF |
1/100 - 1/250.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
Target
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Function
Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. -
Pathway
Protein modification; protein ubiquitination. -
Involvement in disease
Defects in UBA1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2) [MIM:301830]; also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures. -
Sequence similarities
Belongs to the ubiquitin-activating E1 family. -
Post-translational
modificationsISGylated. - Information by UniProt
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Database links
- Entrez Gene: 7317 Human
- Entrez Gene: 22201 Mouse
- Entrez Gene: 314432 Rat
- Omim: 314370 Human
- SwissProt: P22314 Human
- SwissProt: Q02053 Mouse
- SwissProt: Q5U300 Rat
- Unigene: 533273 Human
see all -
Alternative names
- A1S9 antibody
- A1S9 protein antibody
- A1S9T and BN75 temperature sensitivity complementing antibody
see all
Images
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Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)All lanes : Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225) at 1/1000 dilution
Lane 1 : GST tagged Recombinant Human UBA7(Ube1L) protein (Full length, 166 KDa)
Lane 2 : GST tagged Recombinant Human UBA1 protein (Full length, 146 KDa)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 118 kDa
Observed band size: 146,166 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)](https://www.abcam.com/ps/products/181/ab181225/Images/ab181225-214613-anti-e1-ubiquitin-activating-enzyme-antibody-epr14204b-western-blot.jpg)
Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)All lanes : Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225) at 1/10000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 118 kDa
Observed band size: 118 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)](https://www.abcam.com/ps/products/181/ab181225/Images/ab181225-214612-anti-e1-ubiquitin-activating-enzyme-antibody-epr14204b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling E1 Ubiquitin Activating Enzyme with ab181225 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)](https://www.abcam.com/ps/products/181/ab181225/Images/ab181225-214610-anti-e1-ubiquitin-activating-enzyme-antibody-epr14204b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)Immunofluorescent analysis of acetone-fixed HeLa cells labeling E1 Ubiquitin Activating Enzyme with ab181225 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution (red). Counter stained with Dapi (blue).
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![Flow Cytometry (Intracellular) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)](https://www.abcam.com/ps/products/181/ab181225/Images/ab181225-214609-anti-e1-ubiquitin-activating-enzyme-antibody-epr14204b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)Intracellular flow cytometric analysis of2% paraformaldehyde-fixed K562 cells labeling E1 Ubiquitin Activating Enzyme with ab181225 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed byGoat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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![Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)](https://www.abcam.com/ps/products/181/ab181225/Images/ab181225-5-benefits-of-recombinant-antibodies.png)
Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab181225 has been referenced in 1 publication.
- Shi Y et al. Ube2D3 and Ube2N are essential for RIG-I-mediated MAVS aggregation in antiviral innate immunity. Nat Commun 8:15138 (2017). PubMed: 28469175