Anti-E3 ubiquitin-protein ligase MUL1 antibody (ab155511)
Key features and details
- Rabbit polyclonal to E3 ubiquitin-protein ligase MUL1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-E3 ubiquitin-protein ligase MUL1 antibody
See all E3 ubiquitin-protein ligase MUL1 primary antibodies -
Description
Rabbit polyclonal to E3 ubiquitin-protein ligase MUL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to a region within amino acids 1-352 of Human E3 ubiquitin-protein ligase MUL1 (UniProt Q969V5).
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Positive control
- 293T, A431 and Raji cell lysates; A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 40 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 40 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Exhibits weak E3 ubiquitin-protein ligase activity, but preferentially acts as a SUMO E3 ligase at physiological concentrations. Plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. Inhibits cell growth. When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. -
Tissue specificity
Widely expressed with highest levels in the heart, skeletal muscle, placenta, kidney and liver. Barely detectable in colon and thymus. -
Pathway
Protein modification; protein ubiquitination.
Protein modification; protein sumoylation. -
Sequence similarities
Contains 1 RING-type zinc finger. -
Domain
The zinc finger domain is required for E3 ligase activity. -
Cellular localization
Mitochondrion outer membrane. Peroxisome. Transported in mitochondrion-derived vesicles from the mitochondrion to the peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 79594 Human
- Entrez Gene: 68350 Mouse
- Entrez Gene: 298576 Rat
- Omim: 612037 Human
- SwissProt: Q969V5 Human
- SwissProt: Q8VCM5 Mouse
- Unigene: 10101 Human
- Unigene: 103413 Mouse
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Alternative names
- 0610009K11Rik antibody
- AV000801 antibody
- C1orf166 antibody
see all
Images
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Confocal immunofluorescent analysis of methanol-fixed A431 cells labeling E3 ubiquitin-protein ligase MUL1 with ab155511 at 1/500 dilution (Green). Alpha-tubulin filaments are labeled red.
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Anti-E3 ubiquitin-protein ligase MUL1 antibody (ab155511) at 1/2000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 40 kDa
10% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155511 has not yet been referenced specifically in any publications.