Name: Anti-EAAT1 antibody [EPR12686]
Brand: RabMAb
SKU: ab181036
Rating: 5 (3 reviews)

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Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12686] to EAAT1
Suitable for: IHC-P, WB, ICC/IF
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-EAAT1 antibody [EPR12686]
See all EAAT1 primary antibodies
Description

Rabbit monoclonal [EPR12686] to EAAT1
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WB, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Predicted to work with: Monkey
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse brain, rat brain and human cerebellum lysates. IHC-P: Human , Mouse and Rat cerebral cortex tissue sections. ICC/IF: Mouse and rat primary neural / glia cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12686
Isotype

IgG
Research areas
- Neuroscience
- Development
- Neuroscience
- Processes

Alternative Versions
- Anti-EAAT1 antibody [EPR12686] - BSA and Azide free (ab240235)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Mouse brain tissue lysate - total protein (ab30151)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/50 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB		1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
ICC/IF		1/50.

Notes
IHC-P 1/50 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
ICC/IF 1/50.

Function

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
Tissue specificity

Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.
Involvement in disease

Defects in SLC1A3 are the cause of episodic ataxia type 6 (EA6) [MIM:612656]. EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia.
Sequence similarities

Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily.
Post-translational
modifications

Glycosylated.
Cellular localization

Membrane.
Target information above from: UniProt accession P43003 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6507 Human
- Entrez Gene: 20512 Mouse
- Entrez Gene: 29483 Rat
- Omim: 600111 Human
- SwissProt: P43003 Human
- SwissProt: P56564 Mouse
- SwissProt: P24942 Rat
- Unigene: 481918 Human
- Unigene: 204834 Mouse
- Unigene: 34134 Rat
see all
Alternative names
- EA6 antibody
- EAA1_HUMAN antibody
- EAAT1 antibody
- Excitatory amino acid transporter 1 antibody
- FLJ25094 antibody
- GLAST antibody
- GLAST-1 antibody
- GLAST1 antibody
- Glial high affinity glutamate transporter antibody
- glutamate/aspartate transporter, high affinity, sodium-dependent antibody
- High affinity neuronal glutamate transporter antibody
- Slc1a3 antibody
- Sodium dependent glutamate/aspartate transporter antibody
- Sodium-dependent glutamate/aspartate transporter 1 antibody
- Solute carrier family 1 (glial high affinity glutamate transporter) member 3 antibody
- Solute carrier family 1 member 3 antibody
see all

Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)

All lanes : Anti-EAAT1 antibody [EPR12686] (ab181036) at 1/5000 dilution

Lane 1 : Mouse brain lysate boiled
Lane 2 : Mouse brain lysate unboiled

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 59 kDa

Exposure time: 10 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

We recommend not to boil the samples after lysis to get desired WB results.
Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)

All lanes : Anti-EAAT1 antibody [EPR12686] (ab181036) at 1/5000 dilution

Lane 1 : Mouse brain
Lane 2 : Mouse hippocampus
Lane 3 : Rat hippocampus
Lane 4 : Rat brain

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 59 kDa
Additional bands at: 59 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

ab181036 staining EAAT1 in mouse cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

Negative control 1: PBS in place of primary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-EAAT1 antibody [EPR12686] (ab181036)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural / glia cells labelling EAAT1 with ab181036 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in rat primary glia cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-EAAT1 antibody [EPR12686] (ab181036)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural / glia cells labelling EAAT1 with ab181036 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in mouse primary glia cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-EAAT1 antibody [EPR12686] (ab181036)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural / glia cells labelling EAAT1 with ab181036 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in mouse primary neural / glia cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

ab181036 staining EAAT1 in rat cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

ab181036 staining EAAT1 in human glioma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

Negative control 1: PBS in place of primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

ab181036 staining EAAT1 in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

Negative control 1: PBS in place of primary antibody.
Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)

Anti-EAAT1 antibody [EPR12686] (ab181036) at 1/5000 dilution + Human cerebellum at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 59 kDa
Additional bands at: 59 kDa. We are unsure as to the identity of these extra bands.

Blocking and diluting buffer: 5% NFDM/TBST

The band above (100KDa) is dimer
Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)

All lanes : Anti-EAAT1 antibody [EPR12686] (ab181036) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Human cerebellum lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 59 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

Immunohistochemical staining of EAAT1 in paraffin-embedded human brain tissue using ab181036 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-EAAT1 antibody [EPR12686] (ab181036)

Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab181036? Please let us know so that we can cite the reference in this datasheet.

ab181036 has been referenced in 7 publications.

Zheng J et al. GLP-1 improves the supportive ability of astrocytes to neurons by promoting aerobic glycolysis in Alzheimer's disease. Mol Metab 47:101180 (2021). PubMed: 33556642
Zhang X et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873
Sun N et al. DCX and CRABP2 are candidate genes for differential diagnosis between pre-chemotherapy embryonic and alveolar rhabdomyosarcoma in pediatric patients. Pediatr Investig 5:106-111 (2021). PubMed: 34179706
Sipe GO et al. Astrocyte glutamate uptake coordinates experience-dependent, eye-specific refinement in developing visual cortex. Glia 69:1723-1735 (2021). PubMed: 33675674
Sakami S et al. Müller glia phagocytose dead photoreceptor cells in a mouse model of retinal degenerative disease. FASEB J 33:3680-3692 (2019). PubMed: 30462532
Wang R et al. Astrocytic JWA deletion exacerbates dopaminergic neurodegeneration by decreasing glutamate transporters in mice. Cell Death Dis 9:352 (2018). PubMed: 29500411
Liu J et al. Nestin-expressing cell types in the temporal lobe and hippocampus: Morphology, differentiation, and proliferative capacity. Glia 66:62-77 (2018). IHC-P ; Human . PubMed: 28925561

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Application

Western blot

Sample

Bos taurus Cell lysate - whole cell (Lane 1=no sample (negative control) Lane 2=bovine)

Gel Running Conditions

Reduced Denaturing (12%)

Loading amount

20 µg

Specification

Lane 1=no sample (negative control) Lane 2=bovine

Blocking step

No blocking step used for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 28°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Alexis Hruby

Verified customer

Submitted Jul 18 2022

Application

Immunohistochemistry (Frozen sections)

Sample

Common marmoset Tissue sections (Cochlea)

Permeabilization

Specification

Cochlea

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 27 2022

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10mM Citric acid 0.05% Tween20

Sample

Monkey Tissue sections (brain)

Specification

brain

Permeabilization

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 27 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (7)

Customer reviews and Q&As

Western blot abreview for Anti-EAAT1 antibody [EPR12686]

Immunohistochemistry (Frozen sections) abreview for Anti-EAAT1 antibody [EPR12686]

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-EAAT1 [EPR12686] antibody

Post-translational
modifications