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RecombinantRabMAb

Recombinant Anti-EAAT2 antibody [EPR19798] (ab205248)

Submit a review Submit a question References (6)
Western blot - Anti-EAAT2 antibody [EPR19798] (ab205248)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] (ab205248)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] (ab205248)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] (ab205248)
  • Immunohistochemistry (Frozen sections) - Anti-EAAT2 antibody [EPR19798] (ab205248)
  • Immunoprecipitation - Anti-EAAT2 antibody [EPR19798] (ab205248)
  • Anti-EAAT2 antibody [EPR19798] (ab205248)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19798] to EAAT2
  • Suitable for: IHC-Fr, WB, IP, IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

Overview

  • Product name

    Anti-EAAT2 antibody [EPR19798]
    See all EAAT2 primary antibodies

  • Description

    Rabbit monoclonal [EPR19798] to EAAT2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat brain lysates. IHC-P: Mouse and rat striatum tissues. IHC-Fr: Mouse striatum tissue. IP: Mouse brain lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19798

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab205248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
1/500.
WB
1/1000. Detects a band of approximately 65,180 kDa (predicted molecular weight: 62 kDa).
IP
1/30.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
IHC-Fr
1/500.
WB
1/1000. Detects a band of approximately 65,180 kDa (predicted molecular weight: 62 kDa).
IP
1/30.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

  • Sequence similarities

    Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.

  • Post-translational
    modifications

    Glycosylated.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • EAA2_HUMAN antibody
      • EAAT2 antibody
      • Excitatory amino acid transporter 2 antibody
      • Excitotoxic amino acid transporter 2 antibody
      • Glial high affinity glutamate transporter antibody
      • GLT 1 antibody
      • GLT1 antibody
      • Glutamate aspartate transporter II antibody
      • Glutamate transporter 1 antibody
      • Glutamate/aspartate transporter II antibody
      • Slc1a2 antibody
      • Sodium dependent glutamate aspartate transporter 2 antibody
      • Sodium-dependent glutamate/aspartate transporter 2 antibody
      • solute carrier family 1 (glial high affinity glutamate transporter), member 2 antibody
      • Solute carrier family 1 glial high affinity glutamate transporter member 2 antibody
      • Solute carrier family 1 member 2 antibody
      see all

    Images

    • Western blot - Anti-EAAT2 antibody [EPR19798] (ab205248)
      Western blot - Anti-EAAT2 antibody [EPR19798] (ab205248)
      All lanes : Anti-EAAT2 antibody [EPR19798] (ab205248) at 1/2000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Mouse liver lysate
      Lane 3 : Mouse kidney lysate
      Lane 4 : Rat brain lysate
      Lane 5 : Rat liver lysate
      Lane 6 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
      Lane 7 : C6 (Rat glial tumor cell line) whole cell lysate
      Lane 8 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
      Lane 9 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
      Lane 10 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 62 kDa
      Observed band size: 180,65 kDa why is the actual band size different from the predicted?


      Exposure time: 1 second


      Blocking/Dilution buffer: 5% NFDM/TBST.

      1. EAAT2 is a multi-pass membrane protein, the main transporter that clears the excitatory neurotransmitter glutamate in the central nervous system. The bands around 180 kDa are multimers of EAAT2, which is consistent with what have been described in the literatures (PMID: 24569372 & 20193040).

      2. EATT2 is primarily expressed in astrocytes but is also expressed in neurons of the retina and during fetal development(PMID:12176072).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] (ab205248)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] (ab205248)

      Immunohistochemical analysis of paraffin-embedded mouse striatum tissue labeling EAAT2 with ab205248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on mouse striatum is observed [PMID 25391854]. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] (ab205248)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] (ab205248)

      Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling EAAT2 with ab205248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Negative control: no staining on mouse kidney [PMID: 11038258].

      Counter stained with Hematoxylin.

       

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] (ab205248)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT2 antibody [EPR19798] (ab205248)

      Immunohistochemical analysis of paraffin-embedded rat striatum tissue labeling EAAT2 with ab205248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on Rat striatum is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-EAAT2 antibody [EPR19798] (ab205248)
      Immunohistochemistry (Frozen sections) - Anti-EAAT2 antibody [EPR19798] (ab205248)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse striatum tissue labeling EAAT2 with ab205248 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Membrane staining on mouse striatum is observed [PMID: 25391854].  The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077  at 1/1000 dilution.

    • Immunoprecipitation - Anti-EAAT2 antibody [EPR19798] (ab205248)
      Immunoprecipitation - Anti-EAAT2 antibody [EPR19798] (ab205248)

      EAAT2 was immunoprecipitated from 0.35 mg of Mouse brain lysate with ab205248 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab205248 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: Mouse brain lysate, 10μg (Input).

      Lane 2: ab205248 IP in Mouse brain lysate.

      Lane 3: Rabbit IgG,monoclonal [EPR25A]- Isotype Control (ab172730) instead of ab205248 in Mouse brain lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

    • Anti-EAAT2 antibody [EPR19798] (ab205248)
      Anti-EAAT2 antibody [EPR19798] (ab205248)

    References (6)

    Publishing research using ab205248? Please let us know so that we can cite the reference in this datasheet.

    ab205248 has been referenced in 6 publications.

    • Dyomina AV  et al. MTEP, a Selective mGluR5 Antagonist, Had a Neuroprotective Effect but Did Not Prevent the Development of Spontaneous Recurrent Seizures and Behavioral Comorbidities in the Rat Lithium-Pilocarpine Model of Epilepsy. Int J Mol Sci 23:N/A (2022). PubMed: 35008924
    • Postnikova TY  et al. Delayed Impairment of Hippocampal Synaptic Plasticity after Pentylenetetrazole-Induced Seizures in Young Rats. Int J Mol Sci 23:N/A (2022). PubMed: 36362260
    • Zhang X  et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873
    • Servaes S  et al. Neuroreceptor kinetics in rats repeatedly exposed to quinpirole as a model for OCD. PLoS One 14:e0213313 (2019). PubMed: 30845202
    • Zhou X  et al. 5-HT1A Receptor Agonist Promotes Retinal Ganglion Cell Function by Inhibiting OFF-Type Presynaptic Glutamatergic Activity in a Chronic Glaucoma Model. Front Cell Neurosci 13:167 (2019). PubMed: 31130845
    • Zaitsev AV  et al. Ceftriaxone Treatment Affects EAAT2 Expression and Glutamatergic Neurotransmission and Exerts a Weak Anticonvulsant Effect in Young Rats. Int J Mol Sci 20:N/A (2019). PubMed: 31766528

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