Anti-Eagle antibody [8B11DH10] (ab201327)
![Immunohistochemistry (PFA fixed) - Anti-Eagle antibody [8B11DH10] (ab201327)](https://www.abcam.com/ps/products/201/ab201327/Images/ab201327-242847-anti-eagle-antibody-8b11dh10-immunohistochemistry.jpg "Immunohistochemistry (PFA fixed) - Anti-Eagle antibody [8B11DH10] (ab201327)")
Key features and details
- Mouse monoclonal [8B11DH10] to Eagle
- Suitable for: IHC (PFA fixed)
- Reacts with: Drosophila melanogaster
- Isotype: IgG1
Overview
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Product name
Anti-Eagle antibody [8B11DH10] -
Description
Mouse monoclonal [8B11DH10] to Eagle -
Host species
Mouse -
Tested applications
Suitable for: IHC (PFA fixed)more details -
Species reactivity
Reacts with: Drosophila melanogaster -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Drosophila stage 15 embryos.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purification notes
Purity: 95% -
Clonality
Monoclonal -
Clone number
8B11DH10 -
Isotype
IgG1 -
Light chain type
kappa
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC (PFA fixed) |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC (PFA fixed)
Use at an assay dependent concentration. |
Target
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Relevance
May play a critical role in development of the progeny of eagle-positive neuroblasts. -
Cellular localization
Nucleus -
Database links
- Entrez Gene: 40428 Drosophila melanogaster
- SwissProt: P15370 Drosophila melanogaster
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Alternative names
- CG7383 antibody
- eg antibody
- egon antibody
see all
Images
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Immunohistochemistry (PFA fixed) - Anti-Eagle antibody [8B11DH10] (ab201327)
Immunohistochemistry analysis in neuroblast nuclei from Drosophila stage 15 embryos. The cells were fixed in 4% formaldehyde for 25 minutes, permeabilized with 0.1% Triton X-100/PBS, and blocked in 0.1% Triton X-100/PBS, 2.5% Normal Goat Serum, 2.5% Normal Donkey Serum.The Eagle antibody (ab201327) was used at 3.8 µg/mL, overnight at 4°C. The secondary antibody used was Alexa488 conjugated goat anti-mouse at 1/400.
Datasheets and documents
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Datasheet download
References (0)
ab201327 has not yet been referenced specifically in any publications.
