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    products/primary-antibodies/ech1-antibody-3b7cf2ac8-ab123450.pdf

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Anti-ECH1 antibody [3B7CF2AC8] (ab123450)

  • Datasheet
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Western blot - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
  • Immunocytochemistry/ Immunofluorescence - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
  • Flow Cytometry - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)

Key features and details

  • Mouse monoclonal [3B7CF2AC8] to ECH1
  • Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
  • Reacts with: Rat, Cow, Human
  • Isotype: IgG2b

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Recombinant Human ECH1 protein (ab101154)
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Secondary
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-ECH1 antibody [3B7CF2AC8]
    See all ECH1 primary antibodies
  • Description

    Mouse monoclonal [3B7CF2AC8] to ECH1
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Rat, Cow, Human
  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • HDFn cells; Human, Rat and Cow heart homogenates; Human liver homogenate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.5
    Preservative: 0.02% Sodium azide
    Constituent: 99% HEPES buffered saline
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    Purity is near homogeneity as judged by SDS-PAGE. ab123450 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
  • Clonality

    Monoclonal
  • Clone number

    3B7CF2AC8
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Peroxisome
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Recombinant Protein

    • Recombinant Human ECH1 protein (ab101154)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab123450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. 

Notes
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. 

Target

  • Function

    Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.
  • Pathway

    Lipid metabolism; fatty acid beta-oxidation.
  • Sequence similarities

    Belongs to the enoyl-CoA hydratase/isomerase family.
  • Cellular localization

    Mitochondrion. Peroxisome.
  • Target information above from: UniProt accession Q13011 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 507431 Cow
    • Entrez Gene: 1891 Human
    • Entrez Gene: 64526 Rat
    • Omim: 600696 Human
    • SwissProt: Q13011 Human
    • SwissProt: Q62651 Rat
    • Unigene: 196176 Human
    • Unigene: 6148 Rat
    • Alternative names

      • 4)-dienoyl-CoA isomerase antibody
      • 5)-Delta(2 antibody
      • Delta(3 5) Delta(2 4) dienoyl CoA isomerase antibody
      • Delta(3 5) Delta(2 4) dienoyl CoA isomerase mitochondrial antibody
      • Delta(3 antibody
      • Delta3 5 delta2 4 dienoyl CoA isomerase antibody
      • Dienoyl CoA isomerase antibody
      • ECH 1 antibody
      • Ech1 antibody
      • ECH1_HUMAN antibody
      • Enoyl Coenzyme A hydratase 1 antibody
      • Enoyl Coenzyme A hydratase 1 peroxisomal antibody
      • HPXEL antibody
      • mitochondrial antibody
      • MS1034 antibody
      • Peroxisomal enoyl CoA hydratase 1 antibody
      • Peroxisomal enoyl coenzyme A hydratase like protein antibody
      see all

    Images

    • Western blot - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
      Western blot - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
      All lanes : Anti-ECH1 antibody [3B7CF2AC8] (ab123450) at 1 µg/ml

      Lane 1 : Human heart homogenate
      Lane 2 : Human liver homogenate
      Lane 3 : Rat heart homogenate
      Lane 4 : Cow heart homogenate

      Lysates/proteins at 10 µg/ml per lane.

      Secondary
      All lanes : Goat-anti-Mouse HRP

      Predicted band size: 36 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
      Immunocytochemistry/ Immunofluorescence - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
      ab123450 at 1µg/ml staining ECH1 in 4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized HDFn cells by Immunocytochemistry followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). DAPI was used to stain the cell nuclei (blue).
    • Flow Cytometry - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
      Flow Cytometry - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
      ab123450 at 1µg/ml staining ECH1 in HDFn cells by Flow Cytometry (blue) or no primary antibody control (red).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)

      IHC image of ECH1 staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab123450, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab123450? Please let us know so that we can cite the reference in this datasheet.

    ab123450 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Please send a testing discount for testing reactivity with mouse samples by western blotting.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 25 2012

    Answer

    I am very pleased to hear you would like to accept our offer and test ab123450 on western blots of mouse samples. This code will give you one free antibody before the expiration date. To redeem this offer, please submit an Abreview of WB mouse reactivity and include this code in the “Additional Comments” section so we know the Abreview is for this promotion.

    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    We publish both positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the outcome.

    The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Jan 25 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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