Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
Key features and details
- Mouse monoclonal [3B7CF2AC8] to ECH1
- Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
- Reacts with: Rat, Cow, Human
- Isotype: IgG2b
Overview
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Product name
Anti-ECH1 antibody [3B7CF2AC8]
See all ECH1 primary antibodies -
Description
Mouse monoclonal [3B7CF2AC8] to ECH1 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Rat, Cow, Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- HDFn cells; Human, Rat and Cow heart homogenates; Human liver homogenate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
Purity is near homogeneity as judged by SDS-PAGE. ab123450 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
3B7CF2AC8 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab123450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 µg/ml.
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IHC-P |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA. -
Pathway
Lipid metabolism; fatty acid beta-oxidation. -
Sequence similarities
Belongs to the enoyl-CoA hydratase/isomerase family. -
Cellular localization
Mitochondrion. Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 507431 Cow
- Entrez Gene: 1891 Human
- Entrez Gene: 64526 Rat
- Omim: 600696 Human
- SwissProt: Q13011 Human
- SwissProt: Q62651 Rat
- Unigene: 196176 Human
- Unigene: 6148 Rat
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Alternative names
- 4)-dienoyl-CoA isomerase antibody
- 5)-Delta(2 antibody
- Delta(3 5) Delta(2 4) dienoyl CoA isomerase antibody
see all
Images
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All lanes : Anti-ECH1 antibody [3B7CF2AC8] (ab123450) at 1 µg/ml
Lane 1 : Human heart homogenate
Lane 2 : Human liver homogenate
Lane 3 : Rat heart homogenate
Lane 4 : Cow heart homogenate
Lysates/proteins at 10 µg/ml per lane.
Secondary
All lanes : Goat-anti-Mouse HRP
Predicted band size: 36 kDa -
ab123450 at 1µg/ml staining ECH1 in 4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized HDFn cells by Immunocytochemistry followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). DAPI was used to stain the cell nuclei (blue).
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ab123450 at 1µg/ml staining ECH1 in HDFn cells by Flow Cytometry (blue) or no primary antibody control (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECH1 antibody [3B7CF2AC8] (ab123450)
IHC image of ECH1 staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab123450, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab123450 has not yet been referenced specifically in any publications.