Anti-ECH1 antibody [9D11AF3] (ab110294)
![Flow Cytometry - Anti-ECH1 antibody [9D11AF3] (ab110294)](https://www.abcam.com/ps/products/110/ab110294/Images/ECH1-Primary-antibodies-ab110294-2.jpg "Flow Cytometry - Anti-ECH1 antibody [9D11AF3] (ab110294)")
Key features and details
- Mouse monoclonal [9D11AF3] to ECH1
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-ECH1 antibody [9D11AF3]
See all ECH1 primary antibodies -
Description
Mouse monoclonal [9D11AF3] to ECH1 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HDFn cells and HL-60 cells
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General notes
ECH1 is a member of the hydratase/isomerase superfamily involved in fatty acid metabolism. It is localized to peroxisomes and/or mitochondria in different mammals. In humans, levels of ECH1 are modulated by peroxisome proliferators. As the name implies, ECH1 catalyzes the isomerization of cis ?3,5, double bonds in unsaturated acyl-CoAs to yield trans ?2,4-dienoyl CoAs. These are substrates for ?2,4-dienoyl CoA reductase which produces trans ?3 enoyl CoA. This intermediate can then be further transformed by ?3, ?2 - enoyl CoA isomerase to finally yield the trans-2 double bond configured fatty acyl CoA, which can be then directed into the ß-oxidation pathway. Expression of ECH1 is highest in metabolically active tissues such as heart, skeletal muscle, central nervous system, liver and kidney. Recent studies show that it is down regulated in gastric carcinoma and CLL.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Near homogeneity as judged by SDS-PAGE. ab110294 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
9D11AF3 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110294 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 4 µg/ml.
|
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| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
ICC/IF
Use a concentration of 4 µg/ml. |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA. -
Pathway
Lipid metabolism; fatty acid beta-oxidation. -
Sequence similarities
Belongs to the enoyl-CoA hydratase/isomerase family. -
Cellular localization
Mitochondrion. Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 1891 Human
- Omim: 600696 Human
- SwissProt: Q13011 Human
- Unigene: 196176 Human
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Alternative names
- 4)-dienoyl-CoA isomerase antibody
- 5)-Delta(2 antibody
- Delta(3 5) Delta(2 4) dienoyl CoA isomerase antibody
see all
Images
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Flow Cytometry - Anti-ECH1 antibody [9D11AF3] (ab110294)HL-60 cells were stained with 1 µg/mL ab110294 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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![Immunocytochemistry/ Immunofluorescence - Anti-ECH1 antibody [9D11AF3] (ab110294)](https://www.abcam.com/ps/products/110/ab110294/Images/ECH1-Primary-antibodies-ab110294-7.gif)
Immunocytochemistry/ Immunofluorescence - Anti-ECH1 antibody [9D11AF3] (ab110294)Immunocytochemistry image of ab110294 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (9D11AF3, 4 µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (green) AlexaFluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in the mitochondria.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab110294 has not yet been referenced specifically in any publications.