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RecombinantRabMAb

Recombinant Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)

Reviews (1) Submit a question References (11)
Western blot - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
  • Immunoprecipitation - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
  • Indirect ELISA - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
  • Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9710] to EDG2 /LPA-1
  • Suitable for: Indirect ELISA, WB, IHC-P, IP
  • Reacts with: Human, Recombinant fragment

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-EDG2 /LPA-1 antibody [EPR9710]
    See all EDG2 /LPA-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR9710] to EDG2 /LPA-1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Indirect ELISA, WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human, Recombinant fragment

  • Immunogen

    Synthetic peptide within Human EDG2/LPA-1. The exact sequence is proprietary.
    Database link: Q92633
    (Peptide available as ab184693)

  • Positive control

    • Jurkat, HeLa, Caco 2 and A375 cell lysates, paraffin-embedded Human colon tissue, paraffin-embedded Human kidney tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab166903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA
Use at an assay dependent concentration.
WB (1)
1/1000 - 1/5000. Predicted molecular weight: 39 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
Notes
Indirect ELISA
Use at an assay dependent concentration.
WB
1/1000 - 1/5000. Predicted molecular weight: 39 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Function

    Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation.

  • Tissue specificity

    Expressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney. Little or no expression in liver, lung, thymus, or peripheral blood leukocytes.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.

  • Cellular localization

    Cell surface. Cell membrane. Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA co-localizes with RALA in the endocytic vesicles.
  • Information by UniProt

  • Database links

    • Alternative names

      • 5031439C20 antibody
      • AI326300 antibody
      • EDG 2 antibody
      • EDG2 antibody
      • Endothelial differentiation gene 2 antibody
      • Endothelial differentiation, lysophosphatidic acid G protein coupled receptor, 2 antibody
      • GPCR 26 antibody
      • Gpcr26 antibody
      • Gpcr91 antibody
      • GPR26 antibody
      • Kdt2 antibody
      • LPA 1 antibody
      • LPA receptor 1 antibody
      • LPA receptor EDG2 antibody
      • LPA-1 antibody
      • lpA1 antibody
      • Lpar1 antibody
      • LPAR1_HUMAN antibody
      • Lysophosphatidic acid receptor 1 antibody
      • Lysophosphatidic acid receptor Edg-2 antibody
      • Lysophosphatidic acid receptor EDG2 antibody
      • MGC105279 antibody
      • MGC29102 antibody
      • Mrec1.3 antibody
      • rec.1.3 antibody
      • Ventricular zone gene 1 antibody
      • vzg-1 antibody
      • VZG1 antibody
      see all

    Images

    • Western blot - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
      Western blot - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
      All lanes : Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Caco 2 cell lysate
      Lane 4 : A375 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP IgG antibody at 1/2000 dilution

      Predicted band size: 39 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling EDG2 / LPA-1 using ab166903 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling EDG2 / LPA-1 using ab166903 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
      Immunoprecipitation - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
      Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab166903 at 1/10 dilution. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
    • Indirect ELISA - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
      Indirect ELISA - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)

      ELISA analysis of Human EDG2/LPA-1 recombinant protein at 250 ng/mL with ab166903. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

    • Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
      Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)

    References (11)

    Publishing research using ab166903? Please let us know so that we can cite the reference in this datasheet.

    ab166903 has been referenced in 11 publications.

    • Su Z  et al. Downregulation of circLPAR3 inhibits tumor progression and glycolysis by liberating miR-144-3p and upregulating LPCAT1 in oral squamous cell carcinoma. Laryngoscope Investig Otolaryngol 7:425-436 (2022). PubMed: 35434335
    • Wu D  et al. EZH2 promotes the expression of LPA1 by mediating microRNA-139 promoter methylation to accelerate the development of ovarian cancer. Cancer Cell Int 20:551 (2020). PubMed: 33292225
    • Song K  et al. Identification of genes with universally upregulated or downregulated expressions in colorectal cancer. J Gastroenterol Hepatol 34:880-889 (2019). PubMed: 30395690
    • Akoume MY  et al. A Differential Hypofunctionality of Gai Proteins Occurs in Adolescent Idiopathic Scoliosis and Correlates with the Risk of Disease Progression. Sci Rep 9:10074 (2019). PubMed: 31296888
    • Cha YJ & Koo JS Expression of Autotaxin?Lysophosphatidate Signaling-Related Proteins in Breast Cancer with Adipose Stroma. Int J Mol Sci 20:N/A (2019). PubMed: 31035435
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-EDG2 / LPA-1 antibody [EPR9710]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Purified protein (BEL-7404)
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Loading amount
    50 µg
    Treatment
    80 µM actinomycin for 24hrs
    Specification
    BEL-7404
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. guoqing zhu

    Verified customer

    Submitted Jan 26 2016

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