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RecombinantRabMAb

Recombinant Anti-EGFR antibody [SP84] - C-terminal (ab227642)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
  • Flow Cytometry (Intracellular) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
  • Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
  • Western blot - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
  • Anti-EGFR antibody [SP84] - C-terminal (ab227642)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP84] to EGFR - C-terminal
  • Suitable for: ICC/IF, IHC-P, WB, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-EGFR antibody [SP84] - C-terminal
    See all EGFR primary antibodies

  • Description

    Rabbit monoclonal [SP84] to EGFR - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431 EGF treated cell lysate. IHC-P: Human lung squamous cell carcinoma, breast ductal carcinoma, colon adenocarcinoma, lung adenocarcinoma, renal cell carcinoma, stomach adenocarcinoma, liver hepatocellular carcinoma, bladder transitional cell carcinoma, placenta and liver tissues. Flow Cyt: A431 cells. ICC/IF: A431 cells.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/1000.
IHC-P (1)
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.
WB
1/25. Predicted molecular weight: 134 kDa.

Primary antibody incubation for 1 hour at room temperature.
Flow Cyt (Intra)
1/200.

Permeabilize with 90% Methanol (-20 ºC for 30min) after fixation.
Notes
ICC/IF
1/1000.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.
WB
1/25. Predicted molecular weight: 134 kDa.

Primary antibody incubation for 1 hour at room temperature.
Flow Cyt (Intra)
1/200.

Permeabilize with 90% Methanol (-20 ºC for 30min) after fixation.

Target

  • Function

    Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.

  • Tissue specificity

    Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.

  • Involvement in disease

    Lung cancer
    Inflammatory skin and bowel disease, neonatal, 2

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
    Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.

  • Cellular localization

    Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erb-b2 receptor tyrosine kinase 1 antibody
    • ERBB antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • NISBD2 antibody
    • Oncogen ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)

    Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

  • Flow Cytometry (Intracellular) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
    Flow Cytometry (Intracellular) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
    Flow Cytometry analysis of A431(Human epidermoid carcinoma epithelial cell) cells labeling EGFR with purified ab227642 at 1:200 dilution (0.48 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
  • Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
    Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
    Immunocytochemistry/ Immunofluorescence analysis of A431(human epidermoid carcinoma epithelial cell) cells labeling EGFR with purified ab227642 at 1:50 (1.92 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)

    Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)

    Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)

    Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)

    Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)

    Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)

    Formalin-fixed, paraffin-embedded human placenta tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [SP84] - C-terminal (ab227642)

    Formalin-fixed, paraffin-embedded human piver tissue stained for EGFR using ab227642 at 1/100 dilution in immunohistochemical analysis.

  • Western blot - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
    Western blot - Anti-EGFR antibody [SP84] - C-terminal (ab227642)
    Anti-EGFR antibody [SP84] - C-terminal (ab227642) at 1/25 dilution + A431 (human epidermoid carcinoma cell line) EGF treated cell lysate

    Predicted band size: 134 kDa

  • Anti-EGFR antibody [SP84] - C-terminal (ab227642)
    Anti-EGFR antibody [SP84] - C-terminal (ab227642)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab227642? Please let us know so that we can cite the reference in this datasheet.

ab227642 has been referenced in 1 publication.

  • Kwon D  et al. MET exon 14 skipping mutation in triple-negative pulmonary adenocarcinomas and pleomorphic carcinomas: An analysis of intratumoral MET status heterogeneity and clinicopathological characteristics. Lung Cancer 106:131-137 (2017). PubMed: 28285687

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-EGFR antibody [SP84] - C-terminal

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1_Roche
Permeabilization
No
Specification
skin
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Emmanuel Bouchaert

Verified customer

Submitted Feb 28 2022

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com