Anti-EHHADH antibody [7F6AF11] (ab110299)
![Immunoprecipitation - Anti-EHHADH antibody [7F6AF11] (ab110299)](https://www.abcam.com/ps/products/110/ab110299/Images/ab110299-123544-anti-ehhadh-antibody-7f6af11-immunoprecipitation.gif "Immunoprecipitation - Anti-EHHADH antibody [7F6AF11] (ab110299)")
Key features and details
- Mouse monoclonal [7F6AF11] to EHHADH
- Suitable for: IP, ICC
- Reacts with: Human
- Isotype: IgG3
Overview
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Product name
Anti-EHHADH antibody [7F6AF11]
See all EHHADH primary antibodies -
Description
Mouse monoclonal [7F6AF11] to EHHADH -
Host species
Mouse -
Tested applications
Suitable for: IP, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
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Positive control
- ICC: HepG2 cells. IP: Enriched subcellular organelles from human liver.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Near homogeneity as judged by SDS-PAGE (>95% purity). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
7F6AF11 -
Isotype
IgG3 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent dilution.
|
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| ICC |
Use a concentration of 1 µg/ml.
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| Notes |
|---|
|
IP
Use at an assay dependent dilution. |
|
ICC
Use a concentration of 1 µg/ml. |
Target
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Tissue specificity
Liver and kidney. Lower amounts seen in the brain. -
Pathway
Lipid metabolism; fatty acid beta-oxidation. -
Sequence similarities
In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family. -
Post-translational
modificationsAcetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 1962 Human
- Omim: 607037 Human
- SwissProt: Q08426 Human
- Unigene: 429879 Human
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Alternative names
- 3 hydroxyacyl CoA dehydrogenase antibody
- 3,2 trans enoyl CoA isomerase antibody
- 3-hydroxyacyl-CoA dehydrogenase antibody
see all
Images
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Immunoprecipitation - Anti-EHHADH antibody [7F6AF11] (ab110299)
Immonprecipitation analysis of enriched subcellular organelles from human liver, which contains mitochondria and peroxisome. EHHADH immunocaptured using ab110299.
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![Immunocytochemistry - Anti-EHHADH antibody [7F6AF11] (ab110299)](https://www.abcam.com/ps/products/110/ab110299/Images/ab110299-123543-anti-ehhadh-antibody-7f6af11-immunocytochemistry.gif)
Immunocytochemistry - Anti-EHHADH antibody [7F6AF11] (ab110299)Immunocytochemistry image of EHHADH stained Human HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated ab110299 at 1µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in peroxisome.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab110299 has not yet been referenced specifically in any publications.