Name: Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade
Brand: RabMAb
SKU: ab185050
Rating: 5 (4 reviews)

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Western blot - Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18894] to EHMT2/G9A - ChIP Grade
Suitable for: ChIP-sequencing, WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra), ChIC/CUT&RUN-seq
Knockout validated
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Carrier Free

Product name

Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade
See all EHMT2/G9A primary antibodies
Description

Rabbit monoclonal [EPR18894] to EHMT2/G9A - ChIP Grade
Host species

Rabbit
Tested applications

Suitable for: ChIP-sequencing, WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra), ChIC/CUT&RUN-seqmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HEK-293, Jurkat, HepG2, NCCIT, F9, Neuro-2a, LLC1, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates. Human fetal heart and fetal kidney lysates. IHC-P: Human colon, Human gastric adenocarcinoma, mouse liver and rat kidney tissues. ICC/IF: HeLa. Flow Cyt (intra): HeLa cells. IP: HeLa whole cell lysate. ChIP-seq: HeLa cells. ChIC/CUT&RUN-seq: wild-type HeLa cell line (ab255928).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18894
Isotype

IgG
Research areas

Alternative Versions
- Alexa Fluor® 488 Anti-EHMT2/G9A antibody [EPR18894] (ab218359)
- Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade - BSA and Azide free (ab240289)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human EHMT2 (G9A) knockout HeLa cell line (ab265149)
KO cell lysates
- Human EHMT2 (G9A) knockout HeLa cell lysate (ab257080)
Recombinant Protein
- Recombinant human EHMT2/G9A protein (ab80323)
Related Products
- VeriBlot for IP Detection Reagent (HRP) (ab131366)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab185050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ChIP-sequencing		Use 8µg for 10⁷ cells.
WB	(1)	1/1000. Detects a band of approximately 170, 160 kDa (predicted molecular weight: 132 kDa).
IHC-P		1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF	(1)	1/1000.
IP	(1)	1/50.
Flow Cyt (Intra)		1/150.
ChIC/CUT&RUN-seq		Use at an assay dependent concentration.

Notes
ChIP-sequencing Use 8µg for 10⁷ cells.
WB 1/1000. Detects a band of approximately 170, 160 kDa (predicted molecular weight: 132 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/1000.
IP 1/50.
Flow Cyt (Intra) 1/150.
ChIC/CUT&RUN-seq Use at an assay dependent concentration.

Function

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
Tissue specificity

Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.
Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 7 ANK repeats.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.
Domain

The SET domain mediates interaction with WIZ.
The ANK repeats bind H3K9me1 and H3K9me2.
Post-translational
modifications

Methylated at Lys-185; automethylated.
Cellular localization

Nucleus. Chromosome. Associates with euchromatic regions. Does not associate with heterochromatin.
Target information above from: UniProt accession Q96KQ7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 10919 Human
- Entrez Gene: 110147 Mouse
- Entrez Gene: 361798 Rat
- Omim: 604599 Human
- SwissProt: Q96KQ7 Human
- SwissProt: Q9Z148 Mouse
- Unigene: 709218 Human
- Unigene: 35345 Mouse
Alternative names
- Ankyrin repeat containing protein antibody
- Bat 8 antibody
- Bat8 antibody
- C6orf30 antibody
- DKFZp686H08213 antibody
- EHMT 2 antibody
- Ehmt2 antibody
- EHMT2_HUMAN antibody
- Euchromatic histone lysine methyltransferase 2 antibody
- Euchromatic histone lysine N methyltransferase 2 antibody
- Euchromatic histone-lysine N-methyltransferase 2 antibody
- FLJ35547 antibody
- G 9a antibody
- G9 a antibody
- G9a protein antibody
- G9A antibody
- G9A histone methyltransferase antibody
- GAT8 antibody
- H3 K9 HMTase 3 antibody
- H3-K9-HMTase 3 antibody
- Histone H3 K9 methyltransferase 3 antibody
- Histone H3 K9 methyltransferase3 antibody
- Histone H3-K9 methyltransferase 3 antibody
- Histone lysine N methyltransferase antibody
- Histone lysine N methyltransferase EHMT2 antibody
- Histone lysine N methyltransferase, H3 lysine 9 specific 3 antibody
- Histone lysine N methyltransferase, H3 lysine 9 specific3 antibody
- Histone-lysine N-methyltransferase EHMT2 antibody
- HLA B associated transcript 8 antibody
- HLA-B-associated transcript 8 antibody
- KMT 1C antibody
- KMT1 C antibody
- Lysine N methyltransferase 1C antibody
- Lysine N-methyltransferase 1C antibody
- NG 36 antibody
- NG36 antibody
- Protein G9a antibody
see all

Western blot - Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050)

All lanes : Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : EHMT2/G9A knockout HeLa cell lysate
Lane 3 : HEK-293 cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 132 kDa

Lanes 1-4: Merged signal (red and green). Green - ab185050 observed at 160 kDa. Red - loading control ab8245 observed at 37 kDa.

ab185050 Anti-EHMT2/G9A antibody [EPR18894] was shown to specifically react with EHMT2/G9A in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265149 (knockout cell lysate ab257080) was used. Wild-type and EHMT2/G9A knockout samples were subjected to SDS-PAGE. ab185050 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling EHMT2/G9A with ab185050 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on HeLa cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab185050 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
ChIP-sequencing - Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050)

Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ HeLa cells and 8 µg of ab185050 [EPR18894]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.

Additional screenshots of mapped reads can be downloaded here.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling EHMT2/G9A with ab185050 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nucleus staining on rat kidney is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050)

All lanes : Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050) at 1/1000 dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : EHMT2/G9A knockout HAP1 cell lysate
Lane 3 : HEK293 cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 132 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab185050 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab185050 was shown to recognize EHMT2/G9A when EHMT2/G9A knockout samples were used, along with additional cross-reactive bands. Wild-type and EHMT2/G9A knockout samples were subjected to SDS-PAGE. ab185050 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050)

All lanes : Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050) at 1/1000 dilution

Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 4 : NCCIT (Human pluripotent embryonic carcinoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 132 kDa
Observed band size: 160,170 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID 16702210).
Western blot - Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050)

Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050) at 1/1000 dilution + Human fetal heart lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 132 kDa
Observed band size: 160,170 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID 16702210).
Western blot - Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050)

Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050) at 1/1000 dilution + Human fetal kidney lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 132 kDa
Observed band size: 160,170 kDa why is the actual band size different from the predicted?

Exposure time: 8 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID 16702210).
Western blot - Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050)

All lanes : Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050) at 1/1000 dilution

Lane 1 : F9 (Mouse embyro testicular cancer cell line) whole cell lysate
Lane 2 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysate
Lane 3 : LLC1 (Mouse lung carcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 132 kDa
Observed band size: 160,170 kDa why is the actual band size different from the predicted?

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID 16702210).
Western blot - Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050)

All lanes : Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050) at 1/1000 dilution

Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 132 kDa
Observed band size: 160,170 kDa why is the actual band size different from the predicted?

Exposure time: 8 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID 16702210).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling EHMT2/G9A with ab185050 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nucleus staining on epithelial cells of the normal Human colon is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050)

Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma tissue labeling EHMT2/G9A with ab185050 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nucleus staining on tumor cells of the gastric adenocarcinoma is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling EHMT2/G9A with ab185050 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nucleus staining on hepatocytes of the mouse liver is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling EHMT2/G9A with ab185050 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing no staining on MCF7 cell line, as MCF7 cells have a very low level expression of EHMT2/G9A.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab185050 at 1/70 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling EHMT2/G9Awith ab185050 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
ChIC/CUT&RUN sequencing - Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050)

ChIC/CUT&RUN was performed using the ChIC/CUT&RUN pAG-MNAse ab285373. 2.5X10^5 of wild-type HeLa cell line (ab255928) or EHMT2 (G9A) knockout HeLa cell line (ab265149) were used along with 5µg of Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050). Assay Quality Control was conducted using 5µg Anti-CTCF antibody [EPR18253] - ChIP Grade (ab188408) on the same cell lines. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The Negative IgG control is also shown.

Additional screenshots of mapped reads can be downloaded here.
Immunoprecipitation - Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050)

EHMT2/G9A was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab185050 at 1/50 dilution.

Western blot was performed from the immunoprecipitate using ab185050 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/10000 dilution.

Lane 1: HeLa whole cell lysate 10ug (Input).

Lane 2: ab185050 IP in HeLa whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab185050 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (ab185050)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

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Datasheet download

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Publishing research using ab185050? Please let us know so that we can cite the reference in this datasheet.

ab185050 has been referenced in 17 publications.

Cipriano A et al. Epigenetic regulation of Wnt7b expression by the cis-acting long noncoding RNA Lnc-Rewind in muscle stem cells. Elife 10:N/A (2021). PubMed: 33432928
Hou QQ et al. TBC1D3 promotes neural progenitor proliferation by suppressing the histone methyltransferase G9a. Sci Adv 7:N/A (2021). PubMed: 33523893
Barcena-Varela M et al. Epigenetic mechanisms and metabolic reprogramming in fibrogenesis: dual targeting of G9a and DNMT1 for the inhibition of liver fibrosis. Gut 70:388-400 (2021). PubMed: 32327527
Wang H et al. Post-Treatment Sevoflurane Protects Against Hypoxic-Ischemic Brain Injury in Neonatal Rats by Downregulating Histone Methyltransferase G9a and Upregulating Nuclear Factor Erythroid 2-Related Factor 2 (NRF2). Med Sci Monit 27:e930042 (2021). PubMed: 34059615
Xia S et al. SLC7A2 deficiency promotes hepatocellular carcinoma progression by enhancing recruitment of myeloid-derived suppressors cells. Cell Death Dis 12:570 (2021). PubMed: 34108444

View all Publications for this product

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1-4 of 4 Abreviews

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Human Dermal Microvascular Endothelial Cells)

Permeabilization

Yes - 0.1% Triton-X100

Specification

Human Dermal Microvascular Endothelial Cells

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 14 2017

Application

Immunoprecipitation

Sample

Human Cell lysate - whole cell (Dermal microvascular endothelium)

Total protein in input

200 µg

Immuno-precipitation step

Protein A/G

Specification

Dermal microvascular endothelium

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 20 2017

Application

ChIP

Sample

Human Cell lysate - nuclear (B Cells)

Negative control

Isotype IgG

Specification

B Cells

Detection step

Real-time PCR

Type

Cross-linking (X-ChIP)
Duration of cross-linking step: 5 minute(s) and 0 second(s)
Specification of the cross-linking agent: Methanol free Formaldehyde

Positive control

Gene promoter specific primers

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 17 2017

Application

Western blot

Sample

Human Cell lysate - nuclear (Human Dermal Microvascular Endothelial Cells)

Gel Running Conditions

Reduced Denaturing (7.5%)

Loading amount

20 µg

Specification

Human Dermal Microvascular Endothelial Cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 28 2017

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Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

KO cell lines

KO cell lysates

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Domain

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

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SDS download

Datasheet download

Certificate of Compliance

References (17)

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Immunocytochemistry/ Immunofluorescence abreview for Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade

Immunoprecipitation abreview for Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade

ChIP abreview for Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade

Western blot abreview for Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade

Post-translational
modifications