Recombinant Anti-eIF3A antibody [EPR5819(2)] (ab128996)
![Western blot - Anti-eIF3A antibody [EPR5819(2)] (ab128996)](https://www.abcam.com/ps/products/128/ab128996/Images/ab128996-142882-anti-eif3a-antibody-epr58192-western-blot.jpg "Western blot - Anti-eIF3A antibody [EPR5819(2)] (ab128996)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5819(2)] to eIF3A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-eIF3A antibody [EPR5819(2)]
See all eIF3A primary antibodies -
Description
Rabbit monoclonal [EPR5819(2)] to eIF3A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human eIF3A aa 1100-1200. The exact sequence is proprietary.
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Positive control
- BxPC3, 293T, Jurkat, C6, RAW264.7, PC12 and L6 lysates; Human brain tissue; BxPC3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 8.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5819(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 166 kDa).
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
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| ICC/IF |
1/250 - 1/500.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 166 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
ICC/IF
1/250 - 1/500. |
Application notes
Is unsuitable for IP.
Target
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Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed:23766293, PubMed:24357634). In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426). -
Sequence similarities
Belongs to the eIF-3 subunit A family.
Contains 1 PCI domain. -
Post-translational
modificationsPhosphorylated. Phosphorylation is enhanced upon serum stimulation. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 8661 Human
- Entrez Gene: 13669 Mouse
- Entrez Gene: 292148 Rat
- Omim: 602039 Human
- SwissProt: Q14152 Human
- SwissProt: P23116 Mouse
- SwissProt: Q1JU68 Rat
- Unigene: 523299 Human
see all -
Alternative names
- centrosomin homolog antibody
- cytoplasmic protein p167 antibody
- eIF 3 theta antibody
see all
Images
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Western blot - Anti-eIF3A antibody [EPR5819(2)] (ab128996)All lanes : Anti-eIF3A antibody [EPR5819(2)] (ab128996) at 1/1000 dilution
Lane 1 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 5 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 7 : L6 (Rat skeletal muscle cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 166 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3A antibody [EPR5819(2)] (ab128996)](https://www.abcam.com/ps/products/128/ab128996/Images/ab128996-142881-anti-eif3a-antibody-epr58192-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3A antibody [EPR5819(2)] (ab128996)ab128996, at 1/50 dilution, staining eIF3A in paraffin-embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-eIF3A antibody [EPR5819(2)] (ab128996)](https://www.abcam.com/ps/products/128/ab128996/Images/ab128996-142880-anti-eif3a-antibody-epr58192-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-eIF3A antibody [EPR5819(2)] (ab128996)ab128996, at 1/250 dilution, staining eIF3A in BxPC3 cells by Immunofluorescence. -
![OI-RD Scanning - Anti-eIF3A antibody [EPR5819(2)] (ab128996)](https://www.abcam.com/ps/products/128/ab128996/Images/ab128996-142879-anti-eif3a-antibody-epr58192.jpg)
OI-RD Scanning - Anti-eIF3A antibody [EPR5819(2)] (ab128996) -
![Anti-eIF3A antibody [EPR5819(2)] (ab128996)](https://www.abcam.com/ps/products/128/ab128996/Images/ab128996-5-benefits-of-recombinant-antibodies.png)
Anti-eIF3A antibody [EPR5819(2)] (ab128996)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab128996 has been referenced in 6 publications.
- Shi HZ et al. N6-methyladenosine reader YTHDF3 regulates melanoma metastasis via its 'executor'LOXL3. Clin Transl Med 12:e1075 (2022). PubMed: 36324258
- Wang J et al. WTAP promotes myocardial ischemia/reperfusion injury by increasing endoplasmic reticulum stress via regulating m6A modification of ATF4 mRNA. Aging (Albany NY) 13:11135-11149 (2021). PubMed: 33819187
- Jiang SL et al. The Downregulation of eIF3a Contributes to Vemurafenib Resistance in Melanoma by Activating ERK via PPP2R1B. Front Pharmacol 12:720619 (2021). PubMed: 34512348
- Chen T et al. MicroRNA-875-5p inhibits tumor growth and metastasis of hepatocellular carcinoma by targeting eukaryotic translation initiation factor 3 subunit a. Oncol Rep 44:2067-2079 (2020). PubMed: 33000235
- Sonobe Y et al. Translation of dipeptide repeat proteins from the C9ORF72 expanded repeat is associated with cellular stress. Neurobiol Dis 116:155-165 (2018). PubMed: 29792928
- Luo CH et al. HE4 and eIF3a Expression Correlates with Surgical Outcome and Overall Survival in Ovarian Cancer Patients with Secondary Cytoreduction. J Cancer 9:2472-2479 (2018). PubMed: 30026845