Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

Reviews (5) Submit a question References (9)
Western blot - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
  • Flow Cytometry (Intracellular) - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
  • Immunoprecipitation - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
  • Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14301(B)] to eIF4A3
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free PE

Overview

  • Product name

    Anti-eIF4A3 antibody [EPR14301(B)]
    See all eIF4A3 primary antibodies

  • Description

    Rabbit monoclonal [EPR14301(B)] to eIF4A3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Cynomolgus monkey, Xenopus tropicalis

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • MCF7, HeLa, Raji and HepG2 whole cell lysate (ab7900); MCF7 and HeLa cells; Human prostate tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14301(B)

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/160.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (2)
1/1000 - 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF (2)
1/500.
IP (1)
1/30.
Notes
Flow Cyt (Intra)
1/160.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.
IP
1/30.

Target

  • Function

    ATP-dependent RNA helicase. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed.

  • Tissue specificity

    Ubiquitously expressed.

  • Sequence similarities

    Belongs to the DEAD box helicase family. eIF4A subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.

  • Cellular localization

    Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Detected in dendritic layer as well as the nuclear and cytoplasmic (somatic) compartments of neurons. Colocalizes with STAU1 and FMR1 in dendrites.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ATP-dependent RNA helicase DDX48 antibody
    • ATP-dependent RNA helicase eIF4A-3 antibody
    • DDX48 antibody
    • DEAD box protein 48 antibody
    • eIF-4A-III antibody
    • eIF4A-III antibody
    • EIF4A3 antibody
    • eIF4AIII antibody
    • Eukaryotic initiation factor 4A-III antibody
    • Eukaryotic initiation factor 4A-like NUK-34 antibody
    • Eukaryotic translation initiation factor 4A isoform 3 antibody
    • hNMP 265 antibody
    • IF4A3_HUMAN antibody
    • NMP 265 antibody
    • NMP265 antibody
    • Nuclear matrix protein 265 antibody
    • NUK34 antibody
    see all

Images

  • Western blot - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
    Western blot - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
    All lanes : Anti-eIF4A3 antibody [EPR14301(B)] (ab180573) at 1/1000 dilution

    Lane 1 : MCF7 cell line with NFDM
    Lane 2 : Hela cell line with NFDM
    Lane 3 : Raji cell line with NFDM
    Lane 4 : HepG2 cell line with NFDM

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Goat Anti-rabbit HRP at 1/1000 dilution

    Predicted band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

    Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling eIF4A3 with ab180573 at 1/100 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

    Immunofluorescent analysis of 4% paraformaldehyde MCF7 cells labeling eIF4A3 with ab180573 at 1/500 dilution (green), or with Dapi counter stain (blue). Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody, at a dilution of 1/200.

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

    Immunofluorescent analysis of 4% paraformaldehyde Hela cells labeling eIF4A3 with ab180573 at 1/500 dilution (green), or with Dapi counter stain (blue). Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody at a dilution of 1/200.

  • Flow Cytometry (Intracellular) - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
    Flow Cytometry (Intracellular) - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

    Intracellular flow cytometric analysis of 2% paraformaldehydeMCF7 cells labeling eIF4A3 with ab180573 at 1/160 dilution,or a rabbit monoclonal IgG isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution. 

     

     

  • Immunoprecipitation - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
    Immunoprecipitation - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

    Immunoprecipitation of Hela labeling eIF4A3 using ab180573 at a 1/30 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST.



    All lanes : Anti-eIF4A3 antibody [EPR14301(B)] (ab180573) at 1/30 dilution

    Lane 1 : Hela cell lysate with NFDM
    Lane 2 : Negative control with NFDM

    Blocking peptides at 5 % per lane.
  • Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)
    Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

References (9)

Publishing research using ab180573? Please let us know so that we can cite the reference in this datasheet.

ab180573 has been referenced in 9 publications.

  • Qiu M  et al. Plasmacytoma variant translocation 1 stabilized by EIF4A3 promoted malignant biological behaviors of lung adenocarcinoma by generating circular RNA LMNB2. Bioengineered 13:10123-10140 (2022). PubMed: 35435126
  • Sui X  et al. hsa_circ_0101119 facilitates the progression of cervical cancer via an interaction with EIF4A3 to inhibit TCEAL6 expression. Mol Med Rep 24:N/A (2021). PubMed: 34278492
  • Wu Y & Zhao H Circ_0074027 binds to EIF4A3 and promotes gastric cancer progression. Oncol Lett 22:704 (2021). PubMed: 34457059
  • Mao S  et al. Hsa_circ_0004296 inhibits metastasis of prostate cancer by interacting with EIF4A3 to prevent nuclear export of ETS1 mRNA. J Exp Clin Cancer Res 40:336 (2021). PubMed: 34696782
  • Zhu Y  et al. RPL34-AS1-induced RPL34 inhibits cervical cancer cell tumorigenesis via the MDM2-P53 pathway. Cancer Sci 112:1811-1821 (2021). PubMed: 33675124
  • Shadle SC  et al. DUX4-induced bidirectional HSATII satellite repeat transcripts form intranuclear double-stranded RNA foci in human cell models of FSHD. Hum Mol Genet 28:3997-4011 (2019). PubMed: 31630170
  • Ren X  et al. Avian Influenza A Virus Polymerase Recruits Cellular RNA Helicase eIF4A3 to Promote Viral mRNA Splicing and Spliced mRNA Nuclear Export. Front Microbiol 10:1625 (2019). PubMed: 31379779
  • Viphakone N  et al. Co-transcriptional Loading of RNA Export Factors Shapes the Human Transcriptome. Mol Cell 75:310-323.e8 (2019). PubMed: 31104896
  • Shadle SC  et al. DUX4-induced dsRNA and MYC mRNA stabilization activate apoptotic pathways in human cell models of facioscapulohumeral dystrophy. PLoS Genet 13:e1006658 (2017). ICC/IF ; Human . PubMed: 28273136

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-eIF4A3 antibody [EPR14301(B)]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Schmidtea mediterranea Cell lysate - whole cell (planarian cell lysate - whole animal)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
50 µg
Specification
planarian cell lysate - whole animal
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 10 2023

Western blot abreview for Anti-eIF4A3 antibody [EPR14301(B)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse ESC (CCE))
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Mouse ESC (CCE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 26 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com