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RecombinantRabMAb

Recombinant Anti-Elafin/ESI antibody [EPR14631] (ab184972)

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Western blot - Anti-Elafin/ESI antibody [EPR14631] (ab184972)
  • Western blot - Anti-Elafin/ESI antibody [EPR14631] (ab184972)
  • Anti-Elafin/ESI antibody [EPR14631] (ab184972)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14631] to Elafin/ESI
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Elafin/ESI antibody [EPR14631]
    See all Elafin/ESI primary antibodies

  • Description

    Rabbit monoclonal [EPR14631] to Elafin/ESI

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • A431 cell lysate; Human recombinant Elafin/ESI protein.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 12, 14 kDa (predicted molecular weight: 12 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 12, 14 kDa (predicted molecular weight: 12 kDa).

Target

  • Function

    Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

  • Sequence similarities

    Contains 1 WAP domain.

  • Domain

    Consists of two domains: the transglutaminase substrate domain (cementoin moiety) and the elastase inhibitor domain. The transglutaminase substrate domain serves as an anchor to localize elafin covalently to specific sites on extracellular matrix proteins.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cementoin antibody
      • ELAF_HUMAN antibody
      • Elafin antibody
      • Elafin/Skalp antibody
      • Elastase Specific Inhibitor antibody
      • Elastase-specific inhibitor antibody
      • ES 1 antibody
      • ESI antibody
      • Peptidase inhibitor 3 antibody
      • Peptidase inhibitor 3, skin derived antibody
      • PI 3 antibody
      • PI-3 antibody
      • PI3 antibody
      • Pre elafin antibody
      • Protease inhibitor 3 skin derived antibody
      • Protease inhibitor 3, skin derived (SKALP) antibody
      • Protease inhibitor WAP3 antibody
      • SKALP antibody
      • Skin derived Anti leukoproteinase antibody
      • Skin-derived antileukoproteinase antibody
      • Trappin 2 antibody
      • WAP four disulfide core domain 14 antibody
      • WAP four disulfide core domain protein 14 antibody
      • WAP four-disulfide core domain protein 14 antibody
      • WAP3 antibody
      • WFDC14 antibody
      see all

    Images

    • Western blot - Anti-Elafin/ESI antibody [EPR14631] (ab184972)
      Western blot - Anti-Elafin/ESI antibody [EPR14631] (ab184972)
      Anti-Elafin/ESI antibody [EPR14631] (ab184972) at 1/2000 dilution + A431 cell lysate at 20 µg

      Secondary
      Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 12 kDa

    • Western blot - Anti-Elafin/ESI antibody [EPR14631] (ab184972)
      Western blot - Anti-Elafin/ESI antibody [EPR14631] (ab184972)
      Anti-Elafin/ESI antibody [EPR14631] (ab184972) at 1/10000 dilution + Human recombinant His-tagged Elafin/ESI protein (amino acids 23-117) at 0.01 µg

      Secondary
      goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 12 kDa

    • Anti-Elafin/ESI antibody [EPR14631] (ab184972)
      Anti-Elafin/ESI antibody [EPR14631] (ab184972)

    References (0)

    Publishing research using ab184972? Please let us know so that we can cite the reference in this datasheet.

    ab184972 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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