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RecombinantRabMAb

Recombinant Anti-Elastin antibody [EPR20603] - BSA and Azide free (ab238981)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] - BSA and Azide free (ab238981)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] - BSA and Azide free (ab238981)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] - BSA and Azide free (ab238981)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] - BSA and Azide free (ab238981)
  • Anti-Elastin antibody [EPR20603] - BSA and Azide free (ab238981)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20603] to Elastin - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-Elastin antibody [EPR20603] - BSA and Azide free
    See all Elastin primary antibodies

  • Description

    Rabbit monoclonal [EPR20603] to Elastin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Blood vessels in Human lung tissue.

  • General notes

    ab238981 is the carrier-free version of ab213720.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab238981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.

  • Tissue specificity

    Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.

  • Involvement in disease

    Defects in ELN are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Cutis laxa is a rare connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. The skin changes are often accompanied by extracutaneous manifestations, including pulmonary emphysema, bladder diverticula, pulmonary artery stenosis and pyloric stenosis.
    Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
    Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.

  • Sequence similarities

    Belongs to the elastin family.

  • Post-translational
    modifications

    Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
    Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers.
  • Information by UniProt

  • Database links

    • Alternative names

      • Elastin antibody
      • ELN antibody
      • ELN_HUMAN antibody
      • SVAS antibody
      • Tropoelastin antibody
      • WBS antibody
      • WS antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] - BSA and Azide free (ab238981)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] - BSA and Azide free (ab238981)

      Immunohistochemical analysis of paraffin-embedded human breast carcinoma labeling Elastin with ab213720 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) Ready to use. Positive staining on vascular smooth muscle cells of human breast carcinoma.

      Counter stained with Hematoxylin.

      Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) before commencing with IHC staining protocol. The section was incubated with ab213720 at 4°C overnight.

      This data was developed using the same antibody clone in a different buffer formulation (ab213720).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] - BSA and Azide free (ab238981)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] - BSA and Azide free (ab238981)

      Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Elastin with ab213720 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on smooth muscle cells of human testis.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213720).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] - BSA and Azide free (ab238981)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] - BSA and Azide free (ab238981)

      Immunohistochemical analysis of paraffin-embedded artery in human liver tissue labeling Elastin with ab213720 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Extracellular staining on the artery in human liver [PMID: 24461327].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213720).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] - BSA and Azide free (ab238981)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody [EPR20603] - BSA and Azide free (ab238981)

      Immunohistochemical analysis of paraffin-embedded blood vessels in human lung tissue labeling Elastin with ab213720 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on blood vessels in human lung [PMID: 24461327].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213720).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-Elastin antibody [EPR20603] - BSA and Azide free (ab238981)
      Anti-Elastin antibody [EPR20603] - BSA and Azide free (ab238981)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab238981? Please let us know so that we can cite the reference in this datasheet.

    ab238981 has not yet been referenced specifically in any publications.

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