Recombinant Anti-ELKS antibody [EPR13777] (ab180507)
![Western blot - Anti-ELKS antibody [EPR13777] (ab180507)](https://www.abcam.com/ps/products/180/ab180507/Images/ab180507-215604-anti-elks-antibody-epr13777-western-blot.jpg "Western blot - Anti-ELKS antibody [EPR13777] (ab180507)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13777] to ELKS
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ELKS antibody [EPR13777]
See all ELKS primary antibodies -
Description
Rabbit monoclonal [EPR13777] to ELKS -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, HEK293, Jurkat and SW480 cell lysate. SW480 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13777 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 128 kDa).
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| ICC/IF |
1/250.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 128 kDa). |
|
ICC/IF
1/250. |
Target
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Function
Regulatory subunit of the IKK complex. Probably recruits IkappaBalpha/NFKBIA to the complex. May be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. May be involved in vesicle trafficking at the CAZ. May be involved in Rab-6 regulated endosomes to Golgi transport. -
Tissue specificity
Widely expressed. Isoform 2 and isoform 4 are abundantly expressed in brain. Isoform 1 and isoform 3 are predominantly expressed in testis and thyroid, and isoform 1 predominates in other tissues tested. -
Involvement in disease
Thyroid papillary carcinoma (TPC) [MIM:188550]: A common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=The gene represented in this entry may be involved in disease pathogenesis. A chromosomal aberration involving ERC1/RAB6IP2 is found in cause of thyroid papillary carcinomas. Translocation t(10;12)(q11;p13) involving RET. In vitro, isoform 1, isoform 3 and isoform 5 participating in a ERC1-RET fusion protein activate tyrosine-protein kinase activity. -
Sequence similarities
Contains 1 FIP-RBD domain. -
Cellular localization
Cytoplasm. Membrane. Golgi apparatus membrane. In neurons, localized closed to presynaptic membrane. Recruited on Golgi membranes by RAB6A in a GTP-dependent manner. - Information by UniProt
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Database links
- Entrez Gene: 23085 Human
- Entrez Gene: 111173 Mouse
- Entrez Gene: 266806 Rat
- Omim: 607127 Human
- SwissProt: Q8IUD2 Human
- SwissProt: Q99MI1 Mouse
- SwissProt: Q811U3 Rat
- Unigene: 601216 Human
see all -
Alternative names
- Cast 2 antibody
- CAST2 antibody
- CAZ associated structural protein 2 antibody
see all
Images
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Western blot - Anti-ELKS antibody [EPR13777] (ab180507)All lanes : Anti-ELKS antibody [EPR13777] (ab180507) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : SW480 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 128 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-ELKS antibody [EPR13777] (ab180507)](https://www.abcam.com/ps/products/180/ab180507/Images/ab180507-215607-anti-elks-antibody-epr13777-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ELKS antibody [EPR13777] (ab180507)Immunofluorescence analysis of HeLa cells labeling ELKS (red) with ab180507 at 1/250 dilution. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/250 dilution. Nuclei were counterstained with DAPI (blue).
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![Anti-ELKS antibody [EPR13777] (ab180507)](https://www.abcam.com/ps/products/180/ab180507/Images/ab180507-3-benefits-of-recombinant-antibodies.png)
Anti-ELKS antibody [EPR13777] (ab180507)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab180507 has been referenced in 2 publications.
- Kong F et al. Forsythoside B attenuates memory impairment and neuroinflammation via inhibition on NF-?B signaling in Alzheimer's disease. J Neuroinflammation 17:305 (2020). WB ; Mouse . PubMed: 33059746
- Tirosh O et al. The Transcription and Translation Landscapes during Human Cytomegalovirus Infection Reveal Novel Host-Pathogen Interactions. PLoS Pathog 11:e1005288 (2015). WB . PubMed: 26599541