Recombinant Anti-Ellis van Creveld syndrome antibody [EPR10408] (ab171952)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10408] to Ellis van Creveld syndrome
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Ellis van Creveld syndrome antibody [EPR10408]
See all Ellis van Creveld syndrome primary antibodies -
Description
Rabbit monoclonal [EPR10408] to Ellis van Creveld syndrome -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Ellis van Creveld syndrome aa 200-300 (Cysteine residue). The exact sequence is proprietary.
Database link: P57679 -
Positive control
- MCF7, K562, HeLa and 293T cell lysates. HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10408 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 112 kDa.
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ICC/IF |
1/50 - 1/100.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 112 kDa. |
ICC/IF
1/50 - 1/100. |
Target
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Relevance
Ellis van Creveld syndrome is an autosomal recessive disorder characterized by the clinical tetrad of chondrodystrophy, polydactyly, ectodermal dysplasia and cardiac anomalies. Patients manifest short limb dwarfism, short ribs, postaxial polydactyly and dysplastic nails and teeth. Congenital heart defects, most commonly an atrioventricular septal defect, are observed in 60% of affected individuals. Defects in this protein are a cause of Ellis van Creveld syndrome (EVC). -
Cellular localization
Membrane; Single pass membrane protein -
Database links
- Entrez Gene: 2121 Human
- Entrez Gene: 59056 Mouse
- Entrez Gene: 289712 Rat
- Omim: 225500 Human
- Omim: 604831 Human
- SwissProt: P57679 Human
- SwissProt: P57680 Mouse
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Alternative names
- DWF 1 antibody
- DWF1 antibody
- Ellis van Creveld syndrome protein antibody
see all
Images
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All lanes : Anti-Ellis van Creveld syndrome antibody [EPR10408] (ab171952) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 112 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Ellis van Creveld syndrome antibody [EPR10408] (ab171952)
Immunofluorescent analysis of HeLa cells labeling Ellis van Creveld syndrome with ab171952 at 1/50 dilution (red). DAPI nuclear staining (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab171952 has not yet been referenced specifically in any publications.