Recombinant Anti-ELMO1 antibody [EPR12919] (ab174298)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12919] to ELMO1
- Suitable for: ICC/IF, WB, IP, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-ELMO1 antibody [EPR12919]
See all ELMO1 primary antibodies -
Description
Rabbit monoclonal [EPR12919] to ELMO1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IP, Flow Cyt (Intra)more details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12919 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/100 - 1/250.
|
|
WB |
1/10000 - 1/50000. Predicted molecular weight: 84 kDa.
|
|
IP |
1/10 - 1/100.
|
|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
Notes |
---|
ICC/IF
1/100 - 1/250. |
WB
1/10000 - 1/50000. Predicted molecular weight: 84 kDa. |
IP
1/10 - 1/100. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
Target
-
Function
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. -
Tissue specificity
Widely expressed, with a higher expression in the spleen and placenta. -
Sequence similarities
Contains 1 ELMO domain.
Contains 1 PH domain. -
Post-translational
modificationsPhosphorylated by HCK. -
Cellular localization
Cytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK. - Information by UniProt
-
Database links
- Entrez Gene: 9844 Human
- Entrez Gene: 140580 Mouse
- Entrez Gene: 361251 Rat
- Omim: 606420 Human
- SwissProt: Q92556 Human
- SwissProt: Q8BPU7 Mouse
- Unigene: 656638 Human
- Unigene: 342392 Mouse
-
Alternative names
- CED 12 antibody
- Ced 12 homolog 1 antibody
- Ced 12 homolog antibody
see all
Images
-
All lanes : Anti-ELMO1 antibody [EPR12919] (ab174298) at 1/10000 dilution
Lane 1 : Human placenta lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 84 kDa -
Immunocytochemistry/ Immunofluorescence analysis of K562 labeling ELMO1 with ab174298 at 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor® 488); ab150077 at 1/1000 was used as the secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. DAPI was used to counter stain nuclei (blue).
-
Immunocytochemistry/ Immunofluorescence - Anti-ELMO1 antibody [EPR12919] (ab174298)Kim, K. et al Sci Rep. 2017 Jun 30;7(1):4404. doi: 10.1038/s41598-017-04810-6 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
LR73 cells were transfected with the indicated plasmids and stained with Alexa Fluor 488–conjugated phalloidin and anti-Elmo1 antibody.
One day after transfection, cells were washed with cold PBS, fixed in 4% paraformaldehyde for 15 min, and permeabilized with 0.1% Triton X-100 for 5 min. Next, permeabilized cells were blocked with 1% BSA for 30 min and stained with Alex Fluor 594–conjugated phalloidin and anti-Elmo1 antibody for 1 h at room temperature.
(After Figure 5 of Kim et al).
-
Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling ELMO1 with unpurified ab174298 at 1/200 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
-
Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab174298 at 1/10 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab174298 has been referenced in 2 publications.
- Liang Y et al. Downregulation of Dock1 and Elmo1 suppresses the migration and invasion of triple-negative breast cancer epithelial cells through the RhoA/Rac1 pathway. Oncol Lett 16:3481-3488 (2018). PubMed: 30127952
- Kim K et al. Intermolecular steric inhibition of Ephexin4 is relieved by Elmo1. Sci Rep 7:4404 (2017). PubMed: 28667327