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    products/primary-antibodies/elmo1-antibody-epr12919-ab174298.pdf

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RecombinantRabMAb

Recombinant Anti-ELMO1 antibody [EPR12919] (ab174298)

  • Datasheet
  • SDS
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Western blot - Anti-ELMO1 antibody [EPR12919] (ab174298)
  • Immunocytochemistry/ Immunofluorescence - Anti-ELMO1 antibody [EPR12919] (ab174298)
  • Immunocytochemistry/ Immunofluorescence - Anti-ELMO1 antibody [EPR12919] (ab174298)
  • Flow Cytometry (Intracellular) - Anti-ELMO1 antibody [EPR12919] (ab174298)
  • Immunoprecipitation - Anti-ELMO1 antibody [EPR12919] (ab174298)
  • Anti-ELMO1 antibody [EPR12919] (ab174298)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12919] to ELMO1
  • Suitable for: ICC/IF, WB, IP, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

You may also be interested in

Protein
Product image
Recombinant Human ELMO1 protein (ab131839)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Primary
Product image
Anti-RhoA antibody [EPR18134] - Low endotoxin, Azide free (ab219371)

View more associated products

Overview

  • Product name

    Anti-ELMO1 antibody [EPR12919]
    See all ELMO1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12919] to ELMO1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IP, Flow Cyt (Intra)more details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat whole cell lysate (ab7899); human placenta lysate. IP: Jurkat whole cell lysate (ab7899). Flow Cyt (intra): Jurkat cells. ICC/IF: K562 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12919
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Fibronectin
    • Cell Biology
    • Apoptosis
    • Phagocytosis
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Other
    • Cancer
    • Signal transduction
    • G protein signaling
    • Small G proteins
    • Other
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Signal transducers
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Other
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Other
    • Cancer
    • Cell Death
    • Apoptosis
    • Phagocytosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • HRP Anti-ELMO1 antibody [EPR12919] (ab204386)
    • Anti-ELMO1 antibody [EPR12919] - BSA and Azide free (ab222227)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human ELMO1 protein (ab131839)
  • Related Products

    • Recombinant Human ELMO1 protein (ab131839)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab174298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100 - 1/250.
WB
1/10000 - 1/50000. Predicted molecular weight: 84 kDa.
IP
1/10 - 1/100.
Flow Cyt (Intra)
Use at an assay dependent concentration.
Notes
ICC/IF
1/100 - 1/250.
WB
1/10000 - 1/50000. Predicted molecular weight: 84 kDa.
IP
1/10 - 1/100.
Flow Cyt (Intra)
Use at an assay dependent concentration.
Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
  • Tissue specificity

    Widely expressed, with a higher expression in the spleen and placenta.
  • Sequence similarities

    Contains 1 ELMO domain.
    Contains 1 PH domain.
  • Post-translational
    modifications

    Phosphorylated by HCK.
  • Cellular localization

    Cytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK.
  • Target information above from: UniProt accession Q92556 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9844 Human
    • Entrez Gene: 140580 Mouse
    • Entrez Gene: 361251 Rat
    • Omim: 606420 Human
    • SwissProt: Q92556 Human
    • SwissProt: Q8BPU7 Mouse
    • Unigene: 656638 Human
    • Unigene: 342392 Mouse
    • Alternative names

      • CED 12 antibody
      • Ced 12 homolog 1 antibody
      • Ced 12 homolog antibody
      • CED-12 antibody
      • CED12 antibody
      • Ced12 homolog 1 antibody
      • Ced12 homolog antibody
      • ELMO 1 antibody
      • ELMO-1 antibody
      • Elmo1 antibody
      • ELMO1_HUMAN antibody
      • Engulfment and cell motility 1 antibody
      • Engulfment and cell motility protein 1 antibody
      • KIAA0281 antibody
      • MGC126406 antibody
      • Protein ced-12 homolog antibody
      see all

    Images

    • Western blot - Anti-ELMO1 antibody [EPR12919] (ab174298)
      Western blot - Anti-ELMO1 antibody [EPR12919] (ab174298)
      All lanes : Anti-ELMO1 antibody [EPR12919] (ab174298) at 1/10000 dilution

      Lane 1 : Human placenta lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 84 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-ELMO1 antibody [EPR12919] (ab174298)
      Immunocytochemistry/ Immunofluorescence - Anti-ELMO1 antibody [EPR12919] (ab174298)

      Immunocytochemistry/ Immunofluorescence analysis of K562 labeling ELMO1 with ab174298 at 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor® 488); ab150077 at 1/1000 was used as the secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. DAPI was used to counter stain nuclei (blue).

    • Immunocytochemistry/ Immunofluorescence - Anti-ELMO1 antibody [EPR12919] (ab174298)
      Immunocytochemistry/ Immunofluorescence - Anti-ELMO1 antibody [EPR12919] (ab174298)Kim, K. et al Sci Rep. 2017 Jun 30;7(1):4404. doi: 10.1038/s41598-017-04810-6 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      LR73 cells were transfected with the indicated plasmids and stained with Alexa Fluor 488–conjugated phalloidin and anti-Elmo1 antibody.

      One day after transfection, cells were washed with cold PBS, fixed in 4% paraformaldehyde for 15 min, and permeabilized with 0.1% Triton X-100 for 5 min. Next, permeabilized cells were blocked with 1% BSA for 30 min and stained with Alex Fluor 594–conjugated phalloidin and anti-Elmo1 antibody for 1 h at room temperature.

      (After Figure 5 of Kim et al).

      http://creativecommons.org/licenses/by/4.0/.

    • Flow Cytometry (Intracellular) - Anti-ELMO1 antibody [EPR12919] (ab174298)
      Flow Cytometry (Intracellular) - Anti-ELMO1 antibody [EPR12919] (ab174298)

      Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling ELMO1 with unpurified ab174298 at 1/200 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. 

       

       

    • Immunoprecipitation - Anti-ELMO1 antibody [EPR12919] (ab174298)
      Immunoprecipitation - Anti-ELMO1 antibody [EPR12919] (ab174298)

      Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab174298 at 1/10 dilution.

    • Anti-ELMO1 antibody [EPR12919] (ab174298)
      Anti-ELMO1 antibody [EPR12919] (ab174298)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab174298? Please let us know so that we can cite the reference in this datasheet.

    ab174298 has been referenced in 2 publications.

    • Liang Y  et al. Downregulation of Dock1 and Elmo1 suppresses the migration and invasion of triple-negative breast cancer epithelial cells through the RhoA/Rac1 pathway. Oncol Lett 16:3481-3488 (2018). PubMed: 30127952
    • Kim K  et al. Intermolecular steric inhibition of Ephexin4 is relieved by Elmo1. Sci Rep 7:4404 (2017). PubMed: 28667327

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ELMO1 antibody [EPR12919]

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Stomach)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: PH6
    Permeabilization
    Yes - 0.1% triton
    Specification
    Stomach
    Blocking step
    1% BSA, Goat serum, donkey serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 18 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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