Recombinant Anti-Endo G antibody [EP1665Y] (ab76122)
![Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)](https://www.abcam.com/ps/products/76/ab76122/Images/ab76122-258295-anti-endo-g-antibody-ep1665y-western-blot.jpg "Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1665Y] to Endo G
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Endo G antibody [EP1665Y]
See all Endo G primary antibodies -
Description
Rabbit monoclonal [EP1665Y] to Endo G -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide within Human Endo G (C terminal). The exact sequence is proprietary.
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Positive control
- HepG2 whole cell lysate (ab7900)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1665Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
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| Notes |
|---|
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WB
1/2000 - 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa). |
Target
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Function
Cleaves DNA at double-stranded (DG)n.(DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, also has ribonuclease (RNase) and RNase H activities. Capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. -
Sequence similarities
Belongs to the DNA/RNA non-specific endonuclease family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 2021 Human
- Entrez Gene: 13804 Mouse
- Entrez Gene: 362100 Rat
- Omim: 600440 Human
- SwissProt: Q14249 Human
- SwissProt: O08600 Mouse
- Unigene: 745161 Human
- Unigene: 474473 Mouse
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Alternative names
- Endo G antibody
- ENDOG antibody
- Endonuclease G antibody
see all
Images
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Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Endo G HAP1 cell lysate (20 µg)
Lane 3: Mouse liver tissue lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab76122 observed at 29 kDa. Red - loading control, ab7291, observed at 52 kDa.
ab76122 was shown to specifically react with Endo G when Endo G knockout samples were used. Wild-type and Endo G knockout samples were subjected to SDS-PAGE. ab76122 and ab7291 (loading control to alpha tubulin) were diluted 1/2000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)](https://www.abcam.com/ps/products/76/ab76122/Images/ab76122-91461-anti-endo-g-antibody-ep1665y-western-blot.jpg)
Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)Anti-Endo G antibody [EP1665Y] (ab76122) at 1/2000 dilution + HepG2 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted? -
![Anti-Endo G antibody [EP1665Y] (ab76122)](https://www.abcam.com/ps/products/76/ab76122/Images/ab76122-3-benefits-of-recombinant-antibodies.png)
Anti-Endo G antibody [EP1665Y] (ab76122)
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab76122 has been referenced in 6 publications.
- Liu H et al. Copper Induces Oxidative Stress and Apoptosis in the Mouse Liver. Oxid Med Cell Longev 2020:1359164 (2020). PubMed: 32411316
- Blasco N et al. Involvement of the mitochondrial nuclease EndoG in the regulation of cell proliferation through the control of reactive oxygen species. Redox Biol 37:101736 (2020). PubMed: 33032073
- Zhu T et al. TRPM2 channel regulates cytokines production in astrocytes and aggravates brain disorder during lipopolysaccharide-induced endotoxin sepsis. Int Immunopharmacol 75:105836 (2019). PubMed: 31450153
- Wei Q et al. The mitochondrial pathway is involved in sodium fluoride (NaF)-induced renal apoptosis in mice. Toxicol Res (Camb) 7:792-808 (2018). PubMed: 30310657
- Yang S et al. AKT2 Blocks Nucleus Translocation of Apoptosis-Inducing Factor (AIF) and Endonuclease G (EndoG) While Promoting Caspase Activation during Cardiac Ischemia. Int J Mol Sci 18:N/A (2017). PubMed: 28272306
- Pardo R et al. EndoG Knockout Mice Show Increased Brown Adipocyte Recruitment in White Adipose Tissue and Improved Glucose Homeostasis. Endocrinology 157:3873-3887 (2016). Mouse . PubMed: 27547848