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    products/primary-antibodies/endo-g-antibody-ep1665y-ab76122.pdf

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Cell Biology Apoptosis Nucleus DNAses
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Endo G antibody [EP1665Y] (ab76122)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (6)

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Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)
  • Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)
  • Anti-Endo G antibody [EP1665Y] (ab76122)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1665Y] to Endo G
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Secondary
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Anti-CCK1-R antibody (ab75153)

View more associated products

Overview

  • Product name

    Anti-Endo G antibody [EP1665Y]
    See all Endo G primary antibodies
  • Description

    Rabbit monoclonal [EP1665Y] to Endo G
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human Endo G (C terminal). The exact sequence is proprietary.

  • Positive control

    • HepG2 whole cell lysate (ab7900)
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1665Y
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • DNAses
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Other
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Other
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • DNAses
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-Endo G antibody [EP1665Y] - BSA and Azide free (ab247443)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 nuclear extract lysate (ab14660)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000 - 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
Notes
WB
1/2000 - 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Target

  • Function

    Cleaves DNA at double-stranded (DG)n.(DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, also has ribonuclease (RNase) and RNase H activities. Capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.
  • Sequence similarities

    Belongs to the DNA/RNA non-specific endonuclease family.
  • Cellular localization

    Mitochondrion.
  • Target information above from: UniProt accession Q14249 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2021 Human
    • Entrez Gene: 13804 Mouse
    • Entrez Gene: 362100 Rat
    • Omim: 600440 Human
    • SwissProt: Q14249 Human
    • SwissProt: O08600 Mouse
    • Unigene: 745161 Human
    • Unigene: 474473 Mouse
    • Alternative names

      • Endo G antibody
      • ENDOG antibody
      • Endonuclease G antibody
      • Endonuclease G mitochondrial antibody
      • EndonucleaseG antibody
      • FLJ27463 antibody
      • mitochondrial antibody
      • Mitochondrial endonuclease G antibody
      • NUCG_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)
      Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Endo G HAP1 cell lysate (20 µg)
      Lane 3: Mouse liver tissue lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab76122 observed at 29 kDa. Red - loading control, ab7291, observed at 52 kDa.

      ab76122 was shown to specifically react with Endo G when Endo G knockout samples were used. Wild-type and Endo G knockout samples were subjected to SDS-PAGE. ab76122 and ab7291 (loading control to alpha tubulin) were diluted 1/2000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)
      Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)
      Anti-Endo G antibody [EP1665Y] (ab76122) at 1/2000 dilution + HepG2 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 36 kDa
      Observed band size: 35 kDa why is the actual band size different from the predicted?

    • Anti-Endo G antibody [EP1665Y] (ab76122)
      Anti-Endo G antibody [EP1665Y] (ab76122)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (6)

    Publishing research using ab76122? Please let us know so that we can cite the reference in this datasheet.

    ab76122 has been referenced in 6 publications.

    • Liu H  et al. Copper Induces Oxidative Stress and Apoptosis in the Mouse Liver. Oxid Med Cell Longev 2020:1359164 (2020). PubMed: 32411316
    • Blasco N  et al. Involvement of the mitochondrial nuclease EndoG in the regulation of cell proliferation through the control of reactive oxygen species. Redox Biol 37:101736 (2020). PubMed: 33032073
    • Zhu T  et al. TRPM2 channel regulates cytokines production in astrocytes and aggravates brain disorder during lipopolysaccharide-induced endotoxin sepsis. Int Immunopharmacol 75:105836 (2019). PubMed: 31450153
    • Wei Q  et al. The mitochondrial pathway is involved in sodium fluoride (NaF)-induced renal apoptosis in mice. Toxicol Res (Camb) 7:792-808 (2018). PubMed: 30310657
    • Yang S  et al. AKT2 Blocks Nucleus Translocation of Apoptosis-Inducing Factor (AIF) and Endonuclease G (EndoG) While Promoting Caspase Activation during Cardiac Ischemia. Int J Mol Sci 18:N/A (2017). PubMed: 28272306
    • Pardo R  et al. EndoG Knockout Mice Show Increased Brown Adipocyte Recruitment in White Adipose Tissue and Improved Glucose Homeostasis. Endocrinology 157:3873-3887 (2016). Mouse . PubMed: 27547848

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I have purchased ab76122 and it is not working in my western blotting;

    Read More

    Abcam community

    Verified customer

    Asked on Aug 17 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge for one vial of ab7900replacement with the order number 1148322.

    Expect to receive this product on Tuesday August 21. To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Aug 17 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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