Recombinant Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)
![Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)](https://www.abcam.com/ps/products/207/ab207162/Images/ab207162-266559-anti-col18a1-antibody-epr19995-western-blot.jpg "Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19995] to Endostatin/COL18A1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Endostatin/COL18A1 antibody [EPR19995]
See all Endostatin/COL18A1 primary antibodies -
Description
Rabbit monoclonal [EPR19995] to Endostatin/COL18A1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human placenta, seminoma, testis, fetal liver, fetal heart, fetal kidney and fetal spleen lysates; HepG2 whole cell lysate and supernatant.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19995 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab207162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 20, 38, 50, 100, 120, 150, 180, 300 kDa (predicted molecular weight: 178 kDa).
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 20, 38, 50, 100, 120, 150, 180, 300 kDa (predicted molecular weight: 178 kDa). |
Target
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Function
COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.
Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. -
Tissue specificity
Present in multiple organs with highest levels in liver, lung and kidney. -
Involvement in disease
Defects in COL18A1 are a cause of Knobloch syndrome (KNO) [MIM:267750]. KNO is an autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele. -
Sequence similarities
Belongs to the multiplexin collagen family.
Contains 1 FZ (frizzled) domain.
Contains 1 TSP N-terminal (TSPN) domain. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 1306 Human
- Entrez Gene: 80781 Human
- Omim: 120328 Human
- SwissProt: P39059 Human
- SwissProt: P39060 Human
- Unigene: 409034 Human
- Unigene: 517356 Human
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Alternative names
- Alpha 1 collagen type 18 (XVIII)(COL18A1) antibody
- Alpha 1 type XVIII collagen antibody
- Antiangiogenic agent antibody
see all
Images
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Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)All lanes : Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : U937 (Human histiocytic lymphoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
Predicted band size: 178 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Negative control: U937 (PMID: 15451191).
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![Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)](https://www.abcam.com/ps/products/207/ab207162/Images/ab207162-266558-anti-col18a1-antibody-epr19995-western-blot.jpg)
Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)All lanes : Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162) at 1/1000 dilution
Lane 1 : Human seminoma lysate
Lane 2 : Human testis lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Human fetal heart lysate
Lane 5 : Human fetal kidney lysate
Lane 6 : Human fetal spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
Lanes 4-6 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/50000 dilution
Predicted band size: 178 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/3: 1 minute; Lane 2/4-6: 3 minutes.
The MW observed is consistent with the literature (PMID:10716919; PMID: 15451191; PMID: 9687493; PMID: 11581192; PMID: 15545970; PMID: 21555590). -
![Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)](https://www.abcam.com/ps/products/207/ab207162/Images/ab207162-266557-anti-col18a1-antibody-epr19995-western-blot.jpg)
Western blot - Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)All lanes : Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162) at 1/1000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) supernatant
Lysates/proteins at 10 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 178 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Endostatin and NC1 fragments from proteolytic cleavage were secreted into culture supernatant as described by literature (PMID:1590618).
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![Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)](https://www.abcam.com/ps/products/207/ab207162/Images/ab207162-4-benefits-of-recombinant-antibodies.png)
Anti-Endostatin/COL18A1 antibody [EPR19995] (ab207162)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab207162 has been referenced in 1 publication.
- Vaisar T et al. Parallel Murine and Human Plaque Proteomics Reveals Pathways of Plaque Rupture. Circ Res 127:997-1022 (2020). PubMed: 32762496