Recombinant Anti-Endostatin/COL18A1 antibody [EPR22479-279] (ab255601)
![Western blot - Anti-Endostatin/COL18A1 antibody [EPR22479-279] (ab255601)](https://www.abcam.com/ps/products/255/ab255601/Images/ab255601-1-anti-endostatin-col18a1-antibody-epr22479-279-western-blot-mouse.jpg "Western blot - Anti-Endostatin/COL18A1 antibody [EPR22479-279] (ab255601)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22479-279] to Endostatin/COL18A1
- Suitable for: WB
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Endostatin/COL18A1 antibody [EPR22479-279]
See all Endostatin/COL18A1 primary antibodies -
Description
Rabbit monoclonal [EPR22479-279] to Endostatin/COL18A1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse placenta, skin and lung tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22479-279 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab255601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 240, 200, 180, 38, 26, 24, 22 kDa (predicted molecular weight: 182 kDa).
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 240, 200, 180, 38, 26, 24, 22 kDa (predicted molecular weight: 182 kDa). |
Target
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Function
COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.
Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. -
Tissue specificity
Present in multiple organs with highest levels in liver, lung and kidney. -
Involvement in disease
Defects in COL18A1 are a cause of Knobloch syndrome (KNO) [MIM:267750]. KNO is an autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele. -
Sequence similarities
Belongs to the multiplexin collagen family.
Contains 1 FZ (frizzled) domain.
Contains 1 TSP N-terminal (TSPN) domain. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 12819 Mouse
- Entrez Gene: 12822 Mouse
- SwissProt: O35206 Mouse
- SwissProt: P39061 Mouse
- Unigene: 233547 Mouse
- Unigene: 4352 Mouse
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Alternative names
- Alpha 1 collagen type 18 (XVIII)(COL18A1) antibody
- Alpha 1 type XVIII collagen antibody
- Antiangiogenic agent antibody
see all
Images
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Western blot - Anti-Endostatin/COL18A1 antibody [EPR22479-279] (ab255601)All lanes : Anti-Endostatin/COL18A1 antibody [EPR22479-279] (ab255601) at 1/1000 dilution
Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : Mouse placenta tissue lysate
Lane 3 : Mouse skin tissue lysate
Lane 4 : Mouse lung tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 182 kDa
Observed band size: 180,200,22,24,240,26,38 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11581192, 16465514, 10506577).
Negative control: RAW 264.7 (PMID: 27034233).
This antibody reacts with an unidentifiable protein around 75 kDa in lane 1.
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![Anti-Endostatin/COL18A1 antibody [EPR22479-279] (ab255601)](https://www.abcam.com/ps/products/255/ab255601/Images/ab255601-2-benefits-of-recombinant-antibodies.png)
Anti-Endostatin/COL18A1 antibody [EPR22479-279] (ab255601)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab255601 has not yet been referenced specifically in any publications.