Recombinant Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891)
![Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891)](https://www.abcam.com/ps/products/189/ab189891/Images/ab189891-256219-anti-eno1-eno2-eno3-antibody-epr18407-western-blot.jpg "Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18407] to ENO1 + ENO2 + ENO3
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407]
See all ENO1 + ENO2 + ENO3 primary antibodies -
Description
Rabbit monoclonal [EPR18407] to ENO1 + ENO2 + ENO3 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human ENO1, ENO2 and ENO3 full length recombinant proteins; Human fetal heart, fetal kidney and fetal spleen lysates; HeLa, Jurkat, MCF7, A431, C6, RAW 264.7 and NIH/3T3 whole cell lysates; Mouse brain and heart lysates; Rat brain and heart lysates. ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt (intra): NIH/3T3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18407 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/70.
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| ICC/IF |
1/500.
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| WB |
1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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| Notes |
|---|
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Flow Cyt (Intra)
1/70. |
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ICC/IF
1/500. |
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WB
1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
Target
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Relevance
Enolase 1 is a multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. Enolase 2 has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. Enolase 3 appears to have a function in striated muscle development and regeneration. -
Cellular localization
ENO1: Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M-band. Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M-band. ENO2: Cytoplasm. Cell membrane. Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form ENO3: Cytoplasm. Note: Localized to the Z line. Some colocalization with CKM at M-band. -
Database links
- Entrez Gene: 2023 Human
- Entrez Gene: 2026 Human
- Entrez Gene: 2027 Human
- Entrez Gene: 13806 Mouse
- Entrez Gene: 13807 Mouse
- Entrez Gene: 13808 Mouse
- Entrez Gene: 24333 Rat
- Entrez Gene: 24334 Rat
see all -
Alternative names
- 2-phospho-D-glycerate hydro-lyase antibody
- Alpha-enolase antibody
- Beta-enolase antibody
see all
Images
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Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891)All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891) at 1/1000 dilution
Lane 1 : Human ENO1 full length recombinant protein
Lane 2 : Human ENO2 full length recombinant protein
Lane 3 : Human ENO3 full length recombinant protein
Lysates/proteins at 0.02 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Human ENO1 full length recombinant protein (Cat#:ab89248) contains aa1-434. Human ENO2 full length recombinant protein contains aa1-434 with a His-Tag®. Human ENO3 full length recombinant protein(Cat#:ab113127) contains aa1-434 with a His-Tag®. Human ENO2 full length recombinant protein was made in-house.
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![Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891)](https://www.abcam.com/ps/products/189/ab189891/Images/ab189891-256217-anti-eno1-eno2-eno3-antibody-epr18407-western-blot.jpg)
Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891)All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time : Lane 1 and 2: 5 seconds; Lane 3: 15 seconds.
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![Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891)](https://www.abcam.com/ps/products/189/ab189891/Images/ab189891-256216-anti-eno1-eno2-eno3-antibody-epr18407-western-blot.jpg)
Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891)All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891)](https://www.abcam.com/ps/products/189/ab189891/Images/ab189891-256213-anti-eno1-eno2-eno3-antibody-epr18407-western-blot.jpg)
Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891)Lanes 1-4 : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891) at 1/1000 dilution
Lanes 5-7 : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891) at 1/5000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat heart lysate
Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 7 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time : Lane 1-4: 5 seconds; Lane 5-7: 1 second.
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![Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891)](https://www.abcam.com/ps/products/189/ab189891/Images/ab189891-256211-anti-eno1-eno2-eno3-antibody-epr18407-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling ENO1 + ENO2 + ENO3 with ab189891 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing mostly cytoplasmic staining on Hela cells.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab189891 at 1/500 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891)](https://www.abcam.com/ps/products/189/ab189891/Images/ab189891-256210-anti-eno1-eno2-eno3-antibody-epr18407-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling ENO1 + ENO2 + ENO3 with ab189891 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing mostly cytoplasmic staining on NIH/3T3 cells.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody -Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab189891 at 1/500 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891)](https://www.abcam.com/ps/products/189/ab189891/Images/ab189891-256209-anti-eno1-eno2-eno3-antibody-epr18407-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) labeling ENO1 + ENO2 + ENO3 with ab189891 at 1/70 dilution (red) compared with a Rabbit IgG,monoclonal- Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.
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![Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891)](https://www.abcam.com/ps/products/189/ab189891/Images/ab189891-8-benefits-of-recombinant-antibodies.png)
Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab189891 has been referenced in 1 publication.
- Satani N et al. ENOblock Does Not Inhibit the Activity of the Glycolytic Enzyme Enolase. PLoS One 11:e0168739 (2016). PubMed: 28030597