Recombinant Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)
![Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)](https://www.abcam.com/ps/products/250/ab250997/Images/ab250997-446479-anti-eno1-eno2-eno3-antibody-epr18407-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18407] to ENO1 + ENO2 + ENO3 - BSA and Azide free
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free
See all ENO1 + ENO2 + ENO3 primary antibodies -
Description
Rabbit monoclonal [EPR18407] to ENO1 + ENO2 + ENO3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250997 is the carrier-free version of ab189891.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR18407 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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| ICC/IF |
Use at an assay dependent concentration.
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
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ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
Target
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Relevance
Enolase 1 is a multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. Enolase 2 has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. Enolase 3 appears to have a function in striated muscle development and regeneration. -
Cellular localization
ENO1: Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M-band. Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M-band. ENO2: Cytoplasm. Cell membrane. Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form ENO3: Cytoplasm. Note: Localized to the Z line. Some colocalization with CKM at M-band. -
Database links
- Entrez Gene: 2023 Human
- Entrez Gene: 2026 Human
- Entrez Gene: 2027 Human
- Entrez Gene: 13806 Mouse
- Entrez Gene: 13807 Mouse
- Entrez Gene: 13808 Mouse
- Entrez Gene: 24333 Rat
- Entrez Gene: 24334 Rat
see all -
Alternative names
- 2-phospho-D-glycerate hydro-lyase antibody
- Alpha-enolase antibody
- Beta-enolase antibody
see all
Images
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Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891) at 1/1000 dilution
Lane 1 : Human ENO1 full length recombinant protein
Lane 2 : Human ENO2 full length recombinant protein
Lane 3 : Human ENO3 full length recombinant protein
Lysates/proteins at 0.02 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 5 secondsThis data was developed using ab189891, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Human ENO1 full length recombinant protein (ab89248) contains aa1-434. Human ENO2 full length recombinant protein contains aa1-434 with a His-Tag®. Human ENO3 full length recombinant protein (ab113127) contains aa1-434 with a His-Tag®. Human ENO2 full length recombinant protein was made in-house.
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![Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)](https://www.abcam.com/ps/products/250/ab250997/Images/ab250997-445804-anti-eno1-eno2-eno3-antibody-epr18407-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDaThis data was developed using ab189891, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-2: 5 seconds; Lane 3: 15 seconds.
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![Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)](https://www.abcam.com/ps/products/250/ab250997/Images/ab250997-410541-anti-eno1-eno2-eno3-antibody-epr18407-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 3 secondsThis data was developed using ab189891, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)](https://www.abcam.com/ps/products/250/ab250997/Images/ab250997-408225-anti-eno1-eno2-eno3-antibody-epr18407-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)Lanes 1-4 : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891) at 1/1000 dilution
Lanes 5-7 : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] (ab189891) at 1/5000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat heart tissue lysate
Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 7 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDaThis data was developed using ab189891, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-4: 5 seconds; Lanes 5-7: 1 second.
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![Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)](https://www.abcam.com/ps/products/250/ab250997/Images/ab250997-3-anti-eno1-eno2-eno3-antibody-epr18407-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)This data was developed using ab189891, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling ENO1 + ENO2 + ENO3 with ab189891 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing mostly cytoplasmic staining on Hela cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red). The negative controls are as follows:- -ve control 1: ab189891 at 1/500 dilution followed by ab150120 at 1/1000 dilution. -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)](https://www.abcam.com/ps/products/250/ab250997/Images/ab250997-2-anti-eno1-eno2-eno3-antibody-epr18407-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)This data was developed using ab189891, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling ENO1 + ENO2 + ENO3 with ab189891 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing mostly cytoplasmic staining on NIH/3T3 cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody -Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red). The negative controls are as follows:- -ve control 1: ab189891 at 1/500 dilution followed by ab150120 at 1/1000 dilution. -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution. -
![Flow Cytometry (Intracellular) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)](https://www.abcam.com/ps/products/250/ab250997/Images/ab250997-1-anti-eno1-eno2-eno3-antibody-epr18407-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)This data was developed using ab189891, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) labeling ENO1 + ENO2 + ENO3 with ab189891 at 1/70 dilution (red) compared with a Rabbit IgG,monoclonal - Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.
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![Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)](https://www.abcam.com/ps/products/250/ab250997/Images/ab250997-6-benefits-of-recombinant-antibodies.png)
Anti-ENO1 + ENO2 + ENO3 antibody [EPR18407] - BSA and Azide free (ab250997)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250997 has not yet been referenced specifically in any publications.
