Anti-ENO1 antibody [2G2AG11BF8] (ab112994)
![Immunoprecipitation - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)](https://www.abcam.com/ps/products/112/ab112994/Images/ab112994-125709-anti-eno1-antibody-2g2ag11bf8-immunoprecipitation.jpg "Immunoprecipitation - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)")
Key features and details
- Mouse monoclonal [2G2AG11BF8] to ENO1
- Suitable for: IP, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-ENO1 antibody [2G2AG11BF8]
See all ENO1 primary antibodies -
Description
Mouse monoclonal [2G2AG11BF8] to ENO1 -
Host species
Mouse -
Tested applications
Suitable for: IP, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human
Does not react with: Non human primates -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- HeLa and HL60 cells; HeLa, Human Liver and Heart homogenates.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99.98% HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
ab112994 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE (>95%). -
Clonality
Monoclonal -
Clone number
2G2AG11BF8 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
| Notes |
|---|
|
IP
Use at an assay dependent concentration. |
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF
Use a concentration of 5 µg/ml. |
Target
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Function
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. -
Tissue specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
Sequence similarities
Belongs to the enolase family. -
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
Post-translational
modificationsISGylated. -
Cellular localization
Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line. - Information by UniProt
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Database links
- Entrez Gene: 2023 Human
- Omim: 172430 Human
- SwissProt: P06733 Human
- Unigene: 517145 Human
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Alternative names
- 2 phospho D glycerate hydro lyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Alpha enolase antibody
see all
Images
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Immunoprecipitation - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)Immunprecipitation of ENO1 by ab112994.
Staining of ENO1 (47 kDa) from HeLa cell lysate (1), Human liver (2) and Heart (3) homogenates. The identity of the protein was confirmed by mass spectrometry. This gel was stained with colloidal Coomassie blue G. -
![Flow Cytometry - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)](https://www.abcam.com/ps/products/112/ab112994/Images/ab112994-125708-anti-eno1-antibody-2g2ag11bf8-flow-cytometry.jpg)
Flow Cytometry - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)ab112994 at 1ug/ml staining ENO1 in MeOH-fixed HL60 cells by Flow Cytometry(blue). Isotype control antibody (red). -
![Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)](https://www.abcam.com/ps/products/112/ab112994/Images/ab112994-125707-anti-eno1-antibody-2g2ag11bf8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)ab112994 at 5ug/ml staining ENO1 in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green). The target locates mainly to the cytoplasm.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab112994 has been referenced in 1 publication.
- Chen X et al. Interaction between granulin A and enolase 1 attenuates the migration and invasion of human hepatoma cells. Oncotarget 8:30305-30316 (2017). PubMed: 28415822
