Anti-eNOS (phospho S1177) antibody (ab184154)
Key features and details
- Rabbit polyclonal to eNOS (phospho S1177)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-eNOS (phospho S1177) antibody
See all eNOS primary antibodies -
Description
Rabbit polyclonal to eNOS (phospho S1177) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Guinea pig, Pig
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Immunogen
Synthetic peptide within Human eNOS (phospho S1177). The exact sequence is proprietary.
Database link: P29474 -
Positive control
- THP1 whole cell lysate (untreated and treated with 100 nM PMA treatment for 6, 24 or 48 hr), Jurkat, Raji and NCI-H929 whole cell lysate; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 133 kDa.
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| ICC/IF | (1) |
1/100 - 1/1000.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 133 kDa. |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. -
Tissue specificity
Platelets, placenta, liver and kidney. -
Involvement in disease
Variation in NOS3 seem to be associated with susceptibility to coronary spasm. -
Sequence similarities
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. -
Post-translational
modificationsPhosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity. -
Cellular localization
Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity. - Information by UniProt
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Database links
- Entrez Gene: 100135577 Guinea pig
- Entrez Gene: 4846 Human
- Entrez Gene: 397557 Pig
- Omim: 163729 Human
- SwissProt: P97270 Guinea pig
- SwissProt: P29474 Human
- SwissProt: Q28969 Pig
- Unigene: 647092 Human
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Alternative names
- cNOS antibody
- Constitutive NOS antibody
- EC NOS antibody
see all
Images
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Western blot - Anti-eNOS (phospho S1177) antibody (ab184154)All lanes : Anti-eNOS (phospho S1177) antibody (ab184154) at 1/1000 dilution
Lane 1 : HUVEC whole cell lysate
Lane 2 : VEGF (ab9571) treated HUVEC whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit antibody (green) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 133 kDa
Additional bands at: 150 kDa (possible post-translational modification)2e7 HUVEC, grown in serum free medium for 18 hours, were harvested with 0.5 mM EDTA. Cells, collected and resuspended in 10 mL cell culture medium, were stimulated for 2 minutes with 50 ng per mL VEGF (ab9571), after which 30 mL of ice-cold PBS was added. The tubes were spun for 2 min at 1.5k RPM and cell pellets were snap frozen. Control cells were treated in parallel, but without the addition of VEGF.
This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour before being incubated with anti-eNOS (phospho S1177) antibody (ab184154; diluted 1:1000) and loading control (anti-GAPDH antibody [6C5] ab8245; diluted 1:20000) at 4°C overnight. Antibody binding was detected using infrared labelled goat anti-rabbit (green) or anti-mouse (red) antibodies (diluted 1:20000) for 1 hour at room temperature before imaging.
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Western blot - Anti-eNOS (phospho S1177) antibody (ab184154)All lanes : Anti-eNOS (phospho S1177) antibody (ab184154) at 1/500 dilution
Lanes 1 & 3 & 5 : THP1 whole cell lysate
Lane 2 : THP1 whole cell lysate (100 nM PMA treatment for 6 hr)
Lane 4 : THP1 whole cell lysate (100 nM PMA treatment for 24 hr)
Lane 6 : THP1 whole cell lysate (100 nM PMA treatment for 48 hr)
Lysates/proteins at 30 µg per lane.
Predicted band size: 133 kDa5 % SDS-PAGE
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Western blot - Anti-eNOS (phospho S1177) antibody (ab184154)All lanes : Anti-eNOS (phospho S1177) antibody (ab184154) at 1/500 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : NCI-H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 133 kDa5 % SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-eNOS (phospho S1177) antibody (ab184154)Immunofluorescent analysis of methanol fixed HeLa cells labeling eNOS using ab184154 at 1/500 dilution. Image at the right costained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (41)
ab184154 has been referenced in 41 publications.
- Ouyang R et al. FGF21 attenuates high uric acid‑induced endoplasmic reticulum stress, inflammation and vascular endothelial cell dysfunction by activating Sirt1. Mol Med Rep 25:N/A (2022). PubMed: 34850960
- Zhou C et al. Endothelial S1pr2 regulates post-ischemic angiogenesis via AKT/eNOS signaling pathway. Theranostics 12:5172-5188 (2022). PubMed: 35836816
- Zhu Q et al. Nuclear Factor Kappa-B/Homeobox A9-Mediated Modulation of Leucine-Rich Repeat Flightless-Interacting Protein 1 Is Involved in Advanced Glycation End Product-Induced Endothelial Dysfunction. J Vasc Res 58:311-320 (2021). PubMed: 33882503
- Gan YR et al. Dickkopf-1/cysteine-rich angiogenic inducer 61 axis mediates palmitic acid-induced inflammation and apoptosis of vascular endothelial cells. Mol Med Rep 23:N/A (2021). PubMed: 33300071
- Wang T et al. Activation of EphrinB2 Signaling Promotes Adaptive Venous Remodeling in Murine Arteriovenous Fistulae. J Surg Res 262:224-239 (2021). PubMed: 33039109