Anti-Entactin/NID antibody (ab14511)
Key features and details
- Rabbit polyclonal to Entactin/NID
- Suitable for: IHC-Fr, ELISA, ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Entactin/NID antibody
See all Entactin/NID primary antibodies -
Description
Rabbit polyclonal to Entactin/NID -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, ELISA, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration. PubMed: 23409002
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| WB |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 23409002 |
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WB
Use at an assay dependent concentration. |
Target
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Function
Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions. -
Sequence similarities
Contains 6 EGF-like domains.
Contains 4 LDL-receptor class B repeats.
Contains 1 NIDO domain.
Contains 1 nidogen G2 beta-barrel domain.
Contains 1 thyroglobulin type-1 domain. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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Database links
- Entrez Gene: 18073 Mouse
- SwissProt: P10493 Mouse
- Unigene: 4691 Mouse
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Alternative names
- Enactin antibody
- Ent antibody
- Entactin 1 antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (16)
ab14511 has been referenced in 16 publications.
- Vaes N et al. Loss of enteric neuronal Ndrg4 promotes colorectal cancer via increased release of Nid1 and Fbln2. EMBO Rep 22:e51913 (2021). PubMed: 33890711
- Franzka P et al. GMPPA defects cause a neuromuscular disorder with α-dystroglycan hyperglycosylation. J Clin Invest 131:N/A (2021). PubMed: 33755596
- Molè MA et al. Integrin-Mediated Focal Anchorage Drives Epithelial Zippering during Mouse Neural Tube Closure. Dev Cell 52:321-334.e6 (2020). PubMed: 32049039
- Bryan CD et al. Optic cup morphogenesis requires neural crest-mediated basement membrane assembly. Development 147:N/A (2020). PubMed: 31988185
- Carrara N et al. Temporal characterization of optic fissure basement membrane composition suggests nidogen may be an initial target of remodeling. Dev Biol 452:43-54 (2019). PubMed: 31034836
