Recombinant Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)
![Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)](https://www.abcam.com/ps/products/254/ab254994/Images/ab254994-4-anti-entactin-nid-antibody-epr22414-125-immunohistochemistry-frozen-heart-rat.jpg "Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22414-125] to Entactin/NID - BSA and Azide free
- Suitable for: IHC-Fr, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free
See all Entactin/NID primary antibodies -
Description
Rabbit monoclonal [EPR22414-125] to Entactin/NID - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse cardiac tissue and rat cardiac muscle tissue. IHC-Fr: Mouse and rat heart tissue.
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General notes
ab254994 is the carrier-free version of ab254325.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22414-125 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 136 kDa.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC recommended for mouse and rat only. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. |
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 136 kDa. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC recommended for mouse and rat only. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. |
Target
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Function
Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions. -
Sequence similarities
Contains 6 EGF-like domains.
Contains 4 LDL-receptor class B repeats.
Contains 1 NIDO domain.
Contains 1 nidogen G2 beta-barrel domain.
Contains 1 thyroglobulin type-1 domain. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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Database links
- Entrez Gene: 4811 Human
- Entrez Gene: 18073 Mouse
- Entrez Gene: 25494 Rat
- Omim: 131390 Human
- SwissProt: P14543 Human
- SwissProt: P10493 Mouse
- SwissProt: P08460 Rat
- Unigene: 356624 Human
see all -
Alternative names
- Enactin antibody
- Ent antibody
- Entactin 1 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)
Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilized frozen rat heart tissue labeling Entactin/NID with ab254325 at 1/500 dilution (green), followed by an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining on the basement membrane of cardiac muscle (PMID: 12215539) is observed. The nuclear counterstain is DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254325).
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![Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)](https://www.abcam.com/ps/products/254/ab254994/Images/ab254994-3-anti-entactin-nid-antibody-epr22414-125-immunohistochemistry-frozen-heart-mouse.jpg)
Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilized frozen mouse heart tissue labeling Entactin/NID with ab254325 at 1/500 dilution (green), followed by an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining on the basement membrane of cardiac muscle (PMID: 12215539) is observed. The nuclear counterstain is DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254325).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)](https://www.abcam.com/ps/products/254/ab254994/Images/ab254994-2-anti-entactin-nid-antibody-epr22414-125-immunohistochemistry-cardiac-muscle-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Entactin/NID with ab254325 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on basement membrane of rat cardiac muscle (PMID: 10958695). The section was incubated with ab254325 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254325).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)](https://www.abcam.com/ps/products/254/ab254994/Images/ab254994-1-anti-entactin-nid-antibody-epr22414-125-immunohistochemistry-cardiac-muscle-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Entactin/NID with ab254325 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on basement membrane of mouse cardiac muscle (PMID: 10958695). The section was incubated with ab254325 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254325).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)](https://www.abcam.com/ps/products/254/ab254994/Images/ab254994-5-benefits-of-recombinant-antibodies.png)
Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab254994 has not yet been referenced specifically in any publications.
