Recombinant Anti-Entactin/NID antibody [EPR8962(B)] (ab133686)
![Western blot - Anti-Entactin/NID antibody [EPR8962(B)] (ab133686)](https://www.abcam.com/ps/products/133/ab133686/Images/ab133686-147680-anti-entactin-nid-antibody-epr8962b-western-blot.jpg "Western blot - Anti-Entactin/NID antibody [EPR8962(B)] (ab133686)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8962(B)] to Entactin/NID
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Entactin/NID antibody [EPR8962(B)]
See all Entactin/NID primary antibodies -
Description
Rabbit monoclonal [EPR8962(B)] to Entactin/NID -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Entactin/NID aa 50-150 (internal sequence). The exact sequence is proprietary.
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Positive control
- Human fetal heart and fetal lung lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8962(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 136 kDa.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 136 kDa. |
Application notes
Is unsuitable for IHC-P or IP.
Target
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Function
Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions. -
Sequence similarities
Contains 6 EGF-like domains.
Contains 4 LDL-receptor class B repeats.
Contains 1 NIDO domain.
Contains 1 nidogen G2 beta-barrel domain.
Contains 1 thyroglobulin type-1 domain. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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Database links
- Entrez Gene: 4811 Human
- Omim: 131390 Human
- SwissProt: P14543 Human
- Unigene: 356624 Human
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Alternative names
- Enactin antibody
- Ent antibody
- Entactin 1 antibody
see all
Images
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Western blot - Anti-Entactin/NID antibody [EPR8962(B)] (ab133686)All lanes : Anti-Entactin/NID antibody [EPR8962(B)] (ab133686) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 136 kDa -
![Anti-Entactin/NID antibody [EPR8962(B)] (ab133686)](https://www.abcam.com/ps/products/133/ab133686/Images/ab133686-2-benefits-of-recombinant-antibodies.png)
Anti-Entactin/NID antibody [EPR8962(B)] (ab133686)
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab133686 has been referenced in 3 publications.
- Zhou S et al. BET protein inhibitor JQ1 downregulates chromatin accessibility and suppresses metastasis of gastric cancer via inactivating RUNX2/NID1 signaling. Oncogenesis 9:33 (2020). PubMed: 32157097
- Aleckovic M et al. Identification of Nidogen 1 as a lung metastasis protein through secretome analysis. Genes Dev 31:1439-1455 (2017). PubMed: 28827399
- Zhu D et al. Nidogen-1 is a common target of microRNAs MiR-192/215 in the pathogenesis of Hirschsprung's disease. J Neurochem 134:39-46 (2015). PubMed: 25857602