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    products/primary-antibodies/entactinnid-antibody-epr8962b-ab133686.pdf

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Signal Transduction Cytoskeleton / ECM Basal Lamina
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RecombinantRabMAb

Recombinant Anti-Entactin/NID antibody [EPR8962(B)] (ab133686)

  • Datasheet
  • SDS
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Western blot - Anti-Entactin/NID antibody [EPR8962(B)] (ab133686)
  • Anti-Entactin/NID antibody [EPR8962(B)] (ab133686)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8962(B)] to Entactin/NID
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Anti-Entactin/NID antibody [EPR8962(B)]
    See all Entactin/NID primary antibodies
  • Description

    Rabbit monoclonal [EPR8962(B)] to Entactin/NID
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Entactin/NID aa 50-150 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • Human fetal heart and fetal lung lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8962(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Basal Lamina
    • Neuroscience
    • Cell Adhesion Proteins
    • ECM Proteins
    • Cell Biology
    • Cell Cycle
    • Cell differentiation

Associated products

  • Alternative Versions

    • Anti-Entactin/NID antibody [EPR8962(B)] - BSA and Azide free (ab248616)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Entactin/NID protein (ab158997)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 136 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 136 kDa.
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
  • Sequence similarities

    Contains 6 EGF-like domains.
    Contains 4 LDL-receptor class B repeats.
    Contains 1 NIDO domain.
    Contains 1 nidogen G2 beta-barrel domain.
    Contains 1 thyroglobulin type-1 domain.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Target information above from: UniProt accession P14543 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4811 Human
    • Omim: 131390 Human
    • SwissProt: P14543 Human
    • Unigene: 356624 Human
    • Alternative names

      • Enactin antibody
      • Ent antibody
      • Entactin 1 antibody
      • Entactin antibody
      • NID 1 antibody
      • NID antibody
      • NID-1 antibody
      • Nid1 antibody
      • NID1_HUMAN antibody
      • Nidogen (enactin) antibody
      • Nidogen 1 antibody
      • Nidogen antibody
      • Nidogen-1 antibody
      see all

    Images

    • Western blot - Anti-Entactin/NID antibody [EPR8962(B)] (ab133686)
      Western blot - Anti-Entactin/NID antibody [EPR8962(B)] (ab133686)
      All lanes : Anti-Entactin/NID antibody [EPR8962(B)] (ab133686) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : Human fetal lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 136 kDa

    • Anti-Entactin/NID antibody [EPR8962(B)] (ab133686)
      Anti-Entactin/NID antibody [EPR8962(B)] (ab133686)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab133686? Please let us know so that we can cite the reference in this datasheet.

    ab133686 has been referenced in 3 publications.

    • Zhou S  et al. BET protein inhibitor JQ1 downregulates chromatin accessibility and suppresses metastasis of gastric cancer via inactivating RUNX2/NID1 signaling. Oncogenesis 9:33 (2020). PubMed: 32157097
    • Aleckovic M  et al. Identification of Nidogen 1 as a lung metastasis protein through secretome analysis. Genes Dev 31:1439-1455 (2017). PubMed: 28827399
    • Zhu D  et al. Nidogen-1 is a common target of microRNAs MiR-192/215 in the pathogenesis of Hirschsprung's disease. J Neurochem 134:39-46 (2015). PubMed: 25857602

    Customer reviews and Q&As

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