Recombinant Anti-EPCR/CD201 antibody [EPR25647-49] (ab300565)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25647-49] to EPCR/CD201
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-EPCR/CD201 antibody [EPR25647-49]
See all EPCR/CD201 primary antibodies -
Description
Rabbit monoclonal [EPR25647-49] to EPCR/CD201 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Human
Does not react with: Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HUVEC, Ea.Hy926, RKO, Jurkat, bEnd.3, B16-F10 whole cell lysates; HUVEC whole cell lysate treated with Protein Deglycosylation Mix II; human placenta, lung, skin tissue lysates. IHC-P: Human kidney, placenta, colon cancer FFPE tissue sections.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR25647-49 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000.
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
Notes |
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WB
1/1000. |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation. -
Tissue specificity
Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney. -
Post-translational
modificationsN-glycosylated.
A soluble form exists; probably released by a metalloprotease. Seems to have the same activity as the membrane-bound form. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10544 Human
- Entrez Gene: 19124 Mouse
- Omim: 600646 Human
- SwissProt: Q9UNN8 Human
- SwissProt: Q64695 Mouse
- Unigene: 647450 Human
- Unigene: 3243 Mouse
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Alternative names
- Activated protein C receptor antibody
- APC receptor antibody
- APCR antibody
see all
Images
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All lanes : Anti-EPCR/CD201 antibody [EPR25647-49] (ab300565) at 1/1000 dilution
Lane 1 : bEnd.3 (mouse brain endothelioma), whole cell lysate
Lane 2 : B16-F10 (mouse skin melanoma mixture of spindle-shaped and epithelial-like), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 45 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: B16-F10 (PMID: 19188668)
Exposure time: 3 minutes
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All lanes : Anti-EPCR/CD201 antibody [EPR25647-49] (ab300565) at 1/1000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : Human lung tissue lysate
Lane 3 : Human skin tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 45 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The band beneath the target band is supposed to be a soluble form. (PMID: 19806250)
Exposure time: Lane 1: 70 seconds Lane 2, 3: 3 minutes
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All lanes : Anti-EPCR/CD201 antibody [EPR25647-49] (ab300565) at 1/1000 dilution
Lane 1 : Untreated HUVEC whole cell lysate
Lane 2 : HUVEC whole cell lysate treated with Protein Deglycosylation Mix II
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 23,45 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
PROCR is a glycoprotein of approximately 45 kDa and detected as a 23kDa band after treated with Protein Deglycosylation MIX II.
Exposure time: 26 seconds
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All lanes : Anti-EPCR/CD201 antibody [EPR25647-49] (ab300565) at 1/1000 dilution
Lane 1 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
Lane 2 : Ea.Hy926 (human somatic cell hybrid endothelial), whole cell lysate
Lane 3 : RKO (human colon epithelial cell), whole cell lysate
Lane 4 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 45 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: Jurkat (PMID: 7929370)
The band beneath the target band is supposed to be a soluble form. (PMID: 19806250)
Exposure time: 15 seconds
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling EPCR/CD201 with ab300565 at 1/2000 dilution (0.524 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on human colon cancer (PMID: 12110501). The section was incubated with ab300565 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling EPCR/CD201 with ab300565 at 1/2000 dilution (0.524 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on human placenta (PMID:32003123). The section was incubated with ab300565 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling EPCR/CD201 with ab300565 at 1/2000 dilution (0.524 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on endothelial cells of human kidney (PMID:26990852). The section was incubated with ab300565 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab300565 has not yet been referenced specifically in any publications.