Recombinant Anti-Eph receptor A2 antibody [EPR24711-11] (ab273118)
![Western blot - Anti-Eph receptor A2 antibody [EPR24711-11] (ab273118)](https://www.abcam.com/ps/products/273/ab273118/Images/ab273118-484617-antiEph-receptor-A2-antibody-EPR2471111-human-western-blot.jpg "Western blot - Anti-Eph receptor A2 antibody [EPR24711-11] (ab273118)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24711-11] to Eph receptor A2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Eph receptor A2 antibody [EPR24711-11]
See all Eph receptor A2 primary antibodies -
Description
Rabbit monoclonal [EPR24711-11] to Eph receptor A2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate, K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate, Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate, PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate, A549 (human lung carcinoma epithelial cell) whole cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24711-11 -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab273118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 108 kDa.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 108 kDa. |
Target
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Function
Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 and -A5. Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Induces apoptosis in a p53/TP53-independent, caspase-8-dependent manner. -
Tissue specificity
Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g., skin, intestine, lung, and ovary. -
Involvement in disease
Genetic variations in EPHA2 are the cause of susceptibility to cataract cortical age-related type 2 (ARCC2) [MIM:613020]. A developmental punctate opacity common in the cortex and present in most lenses. The cataract is white or cerulean, increases in number with age, but rarely affects vision.
Defects in EPHA2 are the cause of cataract posterior polar type 1 (CTPP1) [MIM:116600]. A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
Post-translational
modificationsActivated by EFNA1 via tyrosine phosphorylation. Phosphorylated residues Tyr-588 and Tyr-594 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-735 and Tyr-930 are required for binding PI3-kinase p85 subunit. These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and endothelial cell migration. They also play a critical role in transducing EPHA2 signaling in vascular endothelial cells during tumor angiogenesis. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1969 Human
- Omim: 176946 Human
- SwissProt: P29317 Human
- Unigene: 171596 Human
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Alternative names
- ARCC2 antibody
- AW545284 antibody
- CTPA antibody
see all
Images
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Western blot - Anti-Eph receptor A2 antibody [EPR24711-11] (ab273118)All lanes : Anti-Eph receptor A2 antibody [EPR24711-11] (ab273118) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate
Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 108 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 81 secondsBlocking / Diluent buffer and concentration: 5% NFDM/TBST
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![Western blot - Anti-Eph receptor A2 antibody [EPR24711-11] (ab273118)](https://www.abcam.com/ps/products/273/ab273118/Images/ab273118-484620-antiEph-receptor-A2-antibody-EPR2471111-western-blot-human.jpg)
Western blot - Anti-Eph receptor A2 antibody [EPR24711-11] (ab273118)All lanes : Anti-Eph receptor A2 antibody [EPR24711-11] (ab273118) at 1/1000 dilution
Lane 1 : Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate.
Lane 2 : Ephrin type-A receptor 2 knockout HeLa (ab264795) whole cell lysate.
Lane 3 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate.
Lane 4 : A549 (human lung carcinoma epithelial cell) whole cell lysate.
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at 1/10000 dilution
Predicted band size: 108 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 81 secondsBlocking / Diluent buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS
Lanes 1-4: Merged signal (red and green). Green - ab273118 observed at 130 kDa. Red - loading control ab8245 observed at 36 kDa.
ab273118 Anti-Ephrin type-A receptor 2 antibody [EPR24711-11] was shown to specifically react with Ephrin type-A receptor 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line (ab264795) was used. Wild-type and Ephrin type-A receptor 2 knockout samples were subjected to SDS-PAGE. ab273118 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging. -
![Anti-Eph receptor A2 antibody [EPR24711-11] (ab273118)](https://www.abcam.com/ps/products/273/ab273118/Images/ab273118-484511-Benefits-of-Recombinant-antibodies.jpg)
Anti-Eph receptor A2 antibody [EPR24711-11] (ab273118)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab273118 has not yet been referenced specifically in any publications.