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  1. Link

    products/primary-antibodies/eph-receptor-a2-antibody-rm-0051-8f21-ab73254.pdf

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Cardiovascular Angiogenesis Ephrins Eph Receptors
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Anti-Eph receptor A2 antibody [RM-0051-8F21] (ab73254)

  • Datasheet
Reviews (7) Submit a question References (4)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor A2 antibody [RM-0051-8F21] (ab73254)
  • Flow Cytometry - Anti-Eph receptor A2 antibody [RM-0051-8F21] (ab73254)

Key features and details

  • Rat monoclonal [RM-0051-8F21] to Eph receptor A2
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant Human Eph receptor A2 protein (ab114439)
Secondary
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Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
Primary
Product image
Anti-Eph receptor A2 antibody [EPR17660-120] (ab185156)

View more associated products

Overview

  • Product name

    Anti-Eph receptor A2 antibody [RM-0051-8F21]
    See all Eph receptor A2 primary antibodies
  • Description

    Rat monoclonal [RM-0051-8F21] to Eph receptor A2
  • Host species

    Rat
  • Specificity

    ab73254 is specific for Eph receptor A2. It does not cross react with recombinant mouse Eph receptors A3, 4, 6, 7, 8, and Eph receptors B1, 2, 3, 4, or 6.
  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant mouse EphA2 extracellular domain

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab73254 was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with recombinant mouse EphA2 extracellular domain. The IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and lyophilized from a 0.2 µm filtered solution.
  • Clonality

    Monoclonal
  • Clone number

    RM-0051-8F21
  • Isotype

    IgG1
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Ephrins
    • Eph Receptors
    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Growth Cone
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Tyrosine Kinase Receptors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Isotype control

    • Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)
  • Recombinant Protein

    • Recombinant Human Eph receptor A2 protein (ab114439)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab73254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100 - 1/200.
Flow Cyt (1)
Use 1µg for 106 cells.

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. 

Notes
IHC-P
1/100 - 1/200.
Flow Cyt
Use 1µg for 106 cells.

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. 

Target

  • Function

    Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 and -A5. Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Induces apoptosis in a p53/TP53-independent, caspase-8-dependent manner.
  • Tissue specificity

    Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g., skin, intestine, lung, and ovary.
  • Involvement in disease

    Genetic variations in EPHA2 are the cause of susceptibility to cataract cortical age-related type 2 (ARCC2) [MIM:613020]. A developmental punctate opacity common in the cortex and present in most lenses. The cataract is white or cerulean, increases in number with age, but rarely affects vision.
    Defects in EPHA2 are the cause of cataract posterior polar type 1 (CTPP1) [MIM:116600]. A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Post-translational
    modifications

    Activated by EFNA1 via tyrosine phosphorylation. Phosphorylated residues Tyr-588 and Tyr-594 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-735 and Tyr-930 are required for binding PI3-kinase p85 subunit. These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and endothelial cell migration. They also play a critical role in transducing EPHA2 signaling in vascular endothelial cells during tumor angiogenesis.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P29317 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1969 Human
    • Entrez Gene: 13836 Mouse
    • Omim: 176946 Human
    • SwissProt: P29317 Human
    • SwissProt: Q03145 Mouse
    • Unigene: 171596 Human
    • Unigene: 2581 Mouse
    • Alternative names

      • ARCC2 antibody
      • AW545284 antibody
      • CTPA antibody
      • CTPP1 antibody
      • CTRCT6 antibody
      • EC 2.7.10.1 antibody
      • Eck antibody
      • Eph receptor A2 antibody
      • EPHA2 antibody
      • EPHA2_HUMAN antibody
      • Ephrin receptor antibody
      • Ephrin receptor EphA2 antibody
      • Ephrin type A receptor 2 antibody
      • Ephrin type-A receptor 2 antibody
      • Epithelial cell kinase antibody
      • Epithelial cell receptor protein tyrosine kinase antibody
      • Myk 2 antibody
      • Myk2 antibody
      • Sek 2 antibody
      • Sek2 antibody
      • Soluble EPHA2 variant 1 antibody
      • Tyrosine protein kinase receptor ECK antibody
      • Tyrosine-protein kinase receptor ECK antibody
      • Tyrosine-protein kinase receptor MPK-5 antibody
      • Tyrosine-protein kinase receptor SEK-2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor A2 antibody [RM-0051-8F21] (ab73254)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor A2 antibody [RM-0051-8F21] (ab73254)

      ab73254 at 1/200 dilution, staining Eph receptor A2 in mouse kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

    • Flow Cytometry - Anti-Eph receptor A2 antibody [RM-0051-8F21] (ab73254)
      Flow Cytometry - Anti-Eph receptor A2 antibody [RM-0051-8F21] (ab73254)

      Overlay histogram showing A431 cells stained with ab73254 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73254, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was goat anti-rat DyLigt 488 (IgG  H+L) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG1 [RTK2071] (ab18412, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (4)

    Publishing research using ab73254? Please let us know so that we can cite the reference in this datasheet.

    ab73254 has been referenced in 4 publications.

    • Jeong MG  et al. Analysis of transient membrane protein interactions by single-molecule diffusional mobility shift assay. Exp Mol Med 53:291-299 (2021). PubMed: 33603128
    • Zong S  et al. A Chinese Herbal Formula Suppresses Colorectal Cancer Migration and Vasculogenic Mimicry Through ROS/HIF-1a/MMP2 Pathway in Hypoxic Microenvironment. Front Pharmacol 11:705 (2020). PubMed: 32499699
    • Wei Q  et al. EphA2-enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer. Mol Med Rep 22:2941-2947 (2020). PubMed: 32945400
    • Ema A  et al. Immunohistochemical analysis of RTKs expression identified HER3 as a prognostic indicator of gastric cancer. Cancer Sci 105:1591-600 (2014). PubMed: 25455899

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-Eph receptor A2 antibody [RM-0051-8F21]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HUES7 ES cells)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    20 µg
    Specification
    HUES7 ES cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 04 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Eph receptor A2 antibody [RM-0051-8F21]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEPG2)
    Specification
    HEPG2
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 18 2015

    Western blot abreview for Anti-Eph receptor A2 antibody [RM-0051-8F21]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEPG2)
    Gel Running Conditions
    Reduced Denaturing (12.5)
    Loading amount
    20 µg
    Specification
    HEPG2
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 13 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Eph receptor A2 antibody [RM-0051-8F21]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    1% serum, 0.1% BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
    Sample
    Mouse Cell (embryonic stem cell)
    Specification
    embryonic stem cell
    Permeabilization
    Yes - 0.1% triton X
    Fixative
    methanol:acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 04 2014

    Western blot abreview for Anti-Eph receptor A2 antibody [RM-0051-8F21]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    1e+006 cells
    Gel Running Conditions
    Non-reduced Denaturing (10% gel)
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic stem cell)
    Specification
    mouse embryonic stem cell
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 11 2014

    Flow Cytometry abreview for Anti-Eph receptor A2 antibody [RM-0051-8F21]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Sample
    Mouse Cell (mouse embryonic stem cell)
    Specification
    mouse embryonic stem cell
    Gating Strategy
    live cells in fsc vs ssc
    Preparation
    Cell harvesting/tissue preparation method: cell dissociation buffer
    Sample buffer: 0.2% BSA, 0.1%NaAz in PBS
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 16 2013

    Immunoprecipitation abreview for Anti-Eph receptor A2 antibody [RM-0051-8F21]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (A431 cancer cell line)
    Total protein in input
    500 µg
    Specification
    A431 cancer cell line
    Immuno-precipitation step
    Other - Trosylactivated Dynabeads
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mrs. Alice Bjerregaard

    Verified customer

    Submitted Dec 18 2009

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