Anti-Eph receptor A2+A3+A4 antibody (ab196899)
Key features and details
- Rabbit polyclonal to Eph receptor A2+A3+A4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Eph receptor A2+A3+A4 antibody
See all Eph receptor A2+A3+A4 primary antibodies -
Description
Rabbit polyclonal to Eph receptor A2+A3+A4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human Eph receptor A2+A3+A4. Synthesized non-phosphopeptide derived from Human EPH receptor A2 + A3 + A4 around the phosphorylation site of tyrosine 588/596 (T-Y(p)-V-D-P). Swiss-Prot: P29317/P29320/P54764
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Positive control
- A549 cells, MCF7, A549, HepG2 cell extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine)
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 108-110 kDa.
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| ICC/IF |
1/100 - 1/500.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 108-110 kDa. |
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ICC/IF
1/100 - 1/500. |
Target
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Relevance
Eph receptor A2 is the receptor for members of the ephrin-A family. It binds to ephrin-A1, -A3, -A4 and -A5. Eph receptor A3 is also a receptor for members of the ephrin-A family. It binds to ephrin-A2, -A3, -A4 and -A5 and is thought to play a role in lymphoid function. Eph receptor A4 is a receptor for members of the ephrin-A family. It binds to ephrin-A1, -A4 and -A5. It binds more poorly to ephrin-A2 and -A3. It may play a role in a signal transduction process involved in hindbrain pattern formation. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 1969 Human
- Entrez Gene: 2042 Human
- Entrez Gene: 2043 Human
- Entrez Gene: 13836 Mouse
- Entrez Gene: 13837 Mouse
- Entrez Gene: 13838 Mouse
- SwissProt: P29317 Human
- SwissProt: P29320 Human
see all -
Alternative names
- ECK antibody
- EPH Receptor A2 antibody
- EPH Receptor A3 antibody
see all
Images
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Western blot - Anti-Eph receptor A2+A3+A4 antibody (ab196899)All lanes : Anti-Eph receptor A2+A3+A4 antibody (ab196899) at 1/500 dilution
Lane 1 : MCF7 cell extract
Lane 2 : MCF7 cell extract with synthesized peptide.
Predicted band size: 108-110 kDa -
Western blot - Anti-Eph receptor A2+A3+A4 antibody (ab196899)All lanes : Anti-Eph receptor A2+A3+A4 antibody (ab196899) at 1/500 dilution
Lane 1 : A549 cell extract with synthesized peptide
Lane 2 : A549 cell extract
Lane 3 : HepG2 cell extract
Predicted band size: 108-110 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor A2+A3+A4 antibody (ab196899)Immunofluorescence analysis of A549 cells labeling Eph receptor A2+A3+A4 with ab196899 at 1/100. Right panel was preincubated with blocking peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab196899 has been referenced in 1 publication.
- Pegg CL et al. Glycoengineering of EphA4 Fc leads to a unique, long-acting and broad spectrum, Eph receptor therapeutic antagonist. Sci Rep 7:6519 (2017). WB ; Human . PubMed: 28747680