Recombinant Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)
![Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)](https://www.abcam.com/ps/products/281/ab281926/Images/ab281926-459939-anti-eph-receptor-a3-antibody-epr24479136-western-blot-carcinoma.jpg "Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24479-136] to Eph receptor A3
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Eph receptor A3 antibody [EPR24479-136]
See all Eph receptor A3 primary antibodies -
Description
Rabbit monoclonal [EPR24479-136] to Eph receptor A3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 22Rv1 and B16-F10 untreated/treated with PNGase F whole cell lysate. His-tagged human EPHA3 recombinant protein.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24479-136 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab281926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 110 kDa.
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| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 110 kDa. |
Target
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Function
Receptor for members of the ephrin-A family. Binds to ephrin-A2, -A3, -A4 and -A5. Could play a role in lymphoid function. -
Tissue specificity
Widely expressed. Highest level in placenta. -
Involvement in disease
Defects in EPHA3 may be a cause of colorectal cancer (CRC) [MIM:114500]. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2042 Human
- Entrez Gene: 13837 Mouse
- Omim: 179611 Human
- SwissProt: P29320 Human
- SwissProt: P29319 Mouse
- Unigene: 123642 Human
- Unigene: 1977 Mouse
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Alternative names
- AW492086 antibody
- Cek4 antibody
- EC 2.7.10.1 antibody
see all
Images
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Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)All lanes : Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926) at 1/1000 dilution
Lane 1 : 22Rv1 (human prostate carcinoma epithelial cell), whole cell lysate
Lane 2 : HL-60 (human Acute Promyelocytic Leukemia promyeloblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 110 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: HL-60 (PMID: 18814179).
Exposure time: 59 seconds.
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![Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)](https://www.abcam.com/ps/products/281/ab281926/Images/ab281926-459943-anti-eph-receptor-a3-antibody-epr24479136-western-blot-carcinoma1.jpg)
Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)All lanes : Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926) at 1/1000 dilution
Lane 1 : 22Rv1 (human prostate carcinoma epithelial cell), whole cell lysate
Lane 2 : 22Rv1 whole cell lysate treated with PNGase F
Lane 3 : B16-F10 (Mouse skin melanoma), whole cell lysate
Lane 4 : B16-F10 whole cell lysate treated with PNGase F
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 110 kDa
Observed band size: 140-150 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
EPHA3 is a glycoprotein and can be de-glycosylated by PNGase F.
The molecular mass observed is consistent with the literature (PMID:28415715).
Exposure times: Lane 1-2: 37 secs; Lane 3-4: 15 secs.
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![Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)](https://www.abcam.com/ps/products/281/ab281926/Images/ab281926-460008-anti-eph-receptor-a3-antibody-epr24479136-western-blot-recombinant.jpg)
Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)All lanes : Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926) at 1/1000 dilution
Lane 1 : His-tagged human EPHA3 recombinant protein at 0.01 µg
Lane 2 : His-tagged human EPHA2 recombinant protein at 0.01 µg
Lane 3 : His-tagged human EPHA4 recombinant protein at 0.01 µg
Lane 4 : His-tagged human EPHA7 recombinant protein at 0.01 µg
Lane 5 : His-tagged human EPHA5 recombinant protein at 0.1 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 110 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure times: Lanes 1-4: 5.5 secs; Lane 5: 26 secs.
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![Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)](https://www.abcam.com/ps/products/281/ab281926/Images/ab281926-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab281926 has not yet been referenced specifically in any publications.