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    products/primary-antibodies/eph-receptor-a3-antibody-epr24479-136-ab281926.pdf

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Cardiovascular Angiogenesis Ephrins Eph Receptors
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RecombinantRabMAb

Recombinant Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)
  • Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)
  • Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)
  • Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24479-136] to Eph receptor A3
  • Suitable for: WB
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Eph receptor A3 antibody [EPR24479-136]
    See all Eph receptor A3 primary antibodies
  • Description

    Rabbit monoclonal [EPR24479-136] to Eph receptor A3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 22Rv1 and B16-F10 untreated/treated with PNGase F whole cell lysate. His-tagged human EPHA3 recombinant protein.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR24479-136
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Ephrins
    • Eph Receptors
    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Growth Cone
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Tyrosine Kinase Receptors

Associated products

  • Alternative Versions

    • Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free (ab281946)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab281926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 110 kDa.
Notes
WB
1/1000. Predicted molecular weight: 110 kDa.
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Target

  • Function

    Receptor for members of the ephrin-A family. Binds to ephrin-A2, -A3, -A4 and -A5. Could play a role in lymphoid function.
  • Tissue specificity

    Widely expressed. Highest level in placenta.
  • Involvement in disease

    Defects in EPHA3 may be a cause of colorectal cancer (CRC) [MIM:114500].
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession P29320 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2042 Human
    • Entrez Gene: 13837 Mouse
    • Omim: 179611 Human
    • SwissProt: P29320 Human
    • SwissProt: P29319 Mouse
    • Unigene: 123642 Human
    • Unigene: 1977 Mouse
    • Alternative names

      • AW492086 antibody
      • Cek4 antibody
      • EC 2.7.10.1 antibody
      • EK4 antibody
      • End3 antibody
      • Eph receptor A3 antibody
      • EPH-like kinase 4 antibody
      • EPH-like tyrosine kinase 1 antibody
      • EPHA3 antibody
      • EPHA3_HUMAN antibody
      • Ephrin receptor EphA3 antibody
      • Ephrin type-A receptor 3 antibody
      • ETK 1 antibody
      • ETK antibody
      • ETK1 antibody
      • HEK 4 antibody
      • HEK antibody
      • HEK4 antibody
      • Human embryo kinase 1 antibody
      • Human embryo kinase antibody
      • Mek4 antibody
      • MGC109882 antibody
      • Receptor tyrosine kinase HEK antibody
      • Testicular tissue protein Li 64 antibody
      • Tyro 4 antibody
      • Tyro4 antibody
      • TYRO4 protein tyrosine kinase antibody
      • Tyrosine protein kinase receptor ETK 1 antibody
      • Tyrosine-protein kinase receptor ETK1 antibody
      • Tyrosine-protein kinase TYRO4 antibody
      see all

    Images

    • Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)
      Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)
      All lanes : Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926) at 1/1000 dilution

      Lane 1 : 22Rv1 (human prostate carcinoma epithelial cell), whole cell lysate
      Lane 2 : HL-60 (human Acute Promyelocytic Leukemia promyeloblast), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 110 kDa
      Observed band size: 150 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Negative control: HL-60 (PMID: 18814179).

      Exposure time: 59 seconds.

    • Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)
      Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)
      All lanes : Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926) at 1/1000 dilution

      Lane 1 : 22Rv1 (human prostate carcinoma epithelial cell), whole cell lysate
      Lane 2 : 22Rv1 whole cell lysate treated with PNGase F
      Lane 3 : B16-F10 (Mouse skin melanoma), whole cell lysate
      Lane 4 : B16-F10 whole cell lysate treated with PNGase F

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 110 kDa
      Observed band size: 140-150 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      EPHA3 is a glycoprotein and can be de-glycosylated by PNGase F.

      The molecular mass observed is consistent with the literature (PMID:28415715).

      Exposure times: Lane 1-2: 37 secs; Lane 3-4: 15 secs.

    • Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)
      Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)
      All lanes : Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926) at 1/1000 dilution

      Lane 1 : His-tagged human EPHA3 recombinant protein at 0.01 µg
      Lane 2 : His-tagged human EPHA2 recombinant protein at 0.01 µg
      Lane 3 : His-tagged human EPHA4 recombinant protein at 0.01 µg
      Lane 4 : His-tagged human EPHA7 recombinant protein at 0.01 µg
      Lane 5 : His-tagged human EPHA5 recombinant protein at 0.1 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 110 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Exposure times: Lanes 1-4: 5.5 secs; Lane 5: 26 secs.

    • Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)
      Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab281926? Please let us know so that we can cite the reference in this datasheet.

    ab281926 has not yet been referenced specifically in any publications.

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