Anti-Eph receptor A4/SEK antibody [M280] (ab157588)
![Western blot - Anti-Eph receptor A4/SEK antibody [M280] (ab157588)](https://www.abcam.com/ps/products/157/ab157588/Images/ab157588-164691-anti-eph-receptor-a4-antibody-m280-western-blot.jpg "Western blot - Anti-Eph receptor A4/SEK antibody [M280] (ab157588)")
Key features and details
- Mouse monoclonal [M280] to Eph receptor A4/SEK
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Eph receptor A4/SEK antibody [M280]
See all Eph receptor A4/SEK primary antibodies -
Description
Mouse monoclonal [M280] to Eph receptor A4/SEK -
Host species
Mouse -
Specificity
The region used as immunogen has less than 50% homology with other EphA and EphB family members. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Chicken
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Immunogen
Recombinant fragment corresponding to Human Eph receptor A4/SEK (N terminal).
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Positive control
- HUVEC and Mouse brain tissue lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
M280 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 95, 120 kDa (predicted molecular weight: 110 kDa).
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 95, 120 kDa (predicted molecular weight: 110 kDa). |
Target
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Function
Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in a signal transduction process involved in hindbrain pattern formation. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
Domain
The protein kinase domain mediates interaction with NGEF/ephexin-1. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 2043 Human
- Entrez Gene: 316539 Rat
- Omim: 602188 Human
- SwissProt: P54764 Human
- Unigene: 371218 Human
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Alternative names
- Cek 8 antibody
- CEK8 antibody
- EK8 antibody
see all
Images
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Western blot - Anti-Eph receptor A4/SEK antibody [M280] (ab157588)All lanes : Anti-Eph receptor A4/SEK antibody [M280] (ab157588) at 1/1000 dilution
Lane 1 : HUVEC cells
Lane 2 : HUVEC cells treated with
pervanadate (1 mM) for 30 min
Predicted band size: 110 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab157588 has been referenced in 1 publication.
- Frejno M et al. Pharmacoproteomic characterisation of human colon and rectal cancer. Mol Syst Biol 13:951 (2017). WB . PubMed: 29101300