Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791)
Key features and details
- Rabbit polyclonal to Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody
See all Eph receptor B1 + Eph receptor B2 primary antibodies -
Description
Rabbit polyclonal to Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) -
Host species
Rabbit -
Specificity
ab61791 detects endogenous levels of EPHB1/2 only when phosphorylated at tyrosine 594 and tyrosine 596.
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Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596).
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Positive control
- Extracts from HepG2 cells, HuvEc cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab61791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
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| ICC/IF | (2) |
1/500 - 1/1000.
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| Notes |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa). |
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ICC/IF
1/500 - 1/1000. |
Target
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Relevance
Ephrin receptors bind members of the ephrin ligand family. They are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. They make up the largest subgroup of the receptor tyrosine kinase (RTK) family. Eph receptor B1 (EphB1) and Eph receptor B2 (EphB2) are receptors for ephrin-B family members. Developing and adult neural tissue express nearly all of the Ephrin receptors and ephrin ligands. Ephrins and ephrin receptors also play a significant role in angiogenesis. EphB2 acts as a tumor suppressor. -
Cellular localization
Membrane; Single-pass type I membrane protein. -
Database links
- Entrez Gene: 2047 Human
- Entrez Gene: 2048 Human
- Entrez Gene: 13844 Mouse
- Entrez Gene: 270190 Mouse
- Entrez Gene: 24338 Rat
- Entrez Gene: 313633 Rat
- Omim: 600600 Human
- Omim: 600997 Human
see all -
Alternative names
- EPH B1 antibody
- EPH B2 antibody
- Eph tyrosine kinase 2 antibody
see all
Images
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Western blot - Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791)This image is courtesy of an Abreview submitted by Thomas JungasAll lanes : Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa whole cell lysate - overexpressing EphB2
Lane 3 : HeLa whole cell lysate - overexpressinf EphB2-kinase dead (K661R)
Lysates/proteins at 100000 cells per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG monoclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 5 minutesAll cells were stimulated with preclustered ephrinb1-Fc chimeric protein.
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Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791)This image is courtesy of an Abreview submitted by Thomas Jungasab61791 staining Eph receptor B1 + Eph receptor B2 (phospho Y594) in HeLa cells (overexpressing EphB2 and stimulated for 20 minutes with preclustered efnb1-Fc chimeric protein) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA + 0.1% Triton X-100) for 2 hour at 25°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody. Red - anti-EphB2 (goat), Grey - DAPI, Green - ab61791.
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Western blot - Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791)All lanes : Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells
Lane 2 : Extracts from HepG2 cells with the immunising phosphopeptide
Predicted band size: 110 kDa
Observed band size: 110 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791)Immunofluorescence analysis of HuvEc cells, using ab61791 at 1/500-1/1000 dilution with (+) or without (-) immunising phosphopeptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab61791 has been referenced in 19 publications.
- Huang Z et al. Key role for EphB2 receptor in kidney fibrosis. Clin Sci (Lond) 135:2127-2142 (2021). PubMed: 34462781
- Wang L et al. Ligand-independent EphB1 signaling mediates TGF-ß-activated CDH2 and promotes lung cancer cell invasion and migration. J Cancer 11:4123-4131 (2020). PubMed: 32368295
- Kischel A et al. Ephrin-B2 paces neuronal production in the developing neocortex. BMC Dev Biol 20:12 (2020). PubMed: 32404061
- Jin F et al. The pathophysiological nature of sarcomeres in trigger points in patients with myofascial pain syndrome: A preliminary study. Eur J Pain 24:1968-1978 (2020). PubMed: 32841448
- Wu Z et al. Role of forward and reverse signaling in Eph receptor and ephrin mediated cell segregation. Exp Cell Res 381:57-65 (2019). PubMed: 31075258