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RecombinantRabMAb

Recombinant Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)
  • Immunohistochemistry (Frozen sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)
  • Immunohistochemistry (Frozen sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)
  • Immunohistochemistry (Frozen sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)
  • Immunohistochemistry (Frozen sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)
  • Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22427-268] to Eph receptor B2 - BSA and Azide free
  • Suitable for: IHC-Fr, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free
    See all Eph receptor B2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22427-268] to Eph receptor B2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NIH/3T3, HT-1080 and 4T1 whole cell lysate. Human, rat and mouse brain tissue lysate. IHC-P: Human, mouse and rat colon tissue. Human bladder cancer tissue. IHC-Fr: Mouse and rat colon tissue.

  • General notes

    ab255274 is the carrier-free version of ab252935.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab255274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).
WB
Use at an assay dependent concentration. Predicted molecular weight: 117 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).
WB
Use at an assay dependent concentration. Predicted molecular weight: 117 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Receptor for members of the ephrin-B family. Phosphorylates ARHGEF15, leading to its ubiquitination and degradation by the proteasome which promotes EFNB1-dependent synapse formation. Can function in aspects of retinal ganglion cell axon guidance to the optic disk even when lacking its tyrosine kinase domain. Acts as a tumor suppressor.

  • Tissue specificity

    Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain.

  • Involvement in disease

    Defects in EPHB2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. Note=EPHB2 mutations have been found in a prostate cancer cell line derived from a brain metastasis.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • cek5 antibody
      • Developmentally regulated EPH related tyrosine kinase antibody
      • DRT antibody
      • EK5 antibody
      • ELK related protein tyrosine kinase antibody
      • Eph receptor B2 antibody
      • EPH tyrosine kinase 3 antibody
      • EPH-like kinase 5 antibody
      • EPHB2 antibody
      • EPHB2_HUMAN antibody
      • Ephrin type B receptor 2 antibody
      • Ephrin type-B receptor 2 antibody
      • EPHT 3 antibody
      • ERK antibody
      • ETECK antibody
      • hEK5 antibody
      • Nuk antibody
      • Prkm 5 antibody
      • Receptor protein tyrosine kinase HEK 5 antibody
      • Renal carcinoma antigen NY-REN-47 antibody
      • Sek 3 antibody
      • Tyro 5 antibody
      • Tyrosine protein kinase receptor CEK 5 antibody
      • Tyrosine protein kinase receptor EPH 3 antibody
      • Tyrosine protein kinase receptor QEK 5 antibody
      • Tyrosine-protein kinase receptor EPH-3 antibody
      • Tyrosine-protein kinase TYRO5 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)

      Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Eph receptor B2 with ab252935 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on the base of the crypts of human colon (PMID: 24151532) is observed. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252935).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)
      Immunohistochemistry (Frozen sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling Eph receptor B2 with ab252935 at 1/250 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on the membrane of epithelial cells, especially located in the basal crypt region (PMID: 19621336) is observed. Counterstained with DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252935).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)

      Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Eph receptor B2 with ab252935 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on rat colon (PMID: 24151532) is observed. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252935).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)
      Immunohistochemistry (Frozen sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat colon tissue labeling Eph receptor B2 with ab252935 at 1/250 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on the membrane of epithelial cells, especially located in the basal crypt region (PMID: 19621336) is observed. Counterstained with DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252935).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)

      Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Eph receptor B2 with ab252935 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on mouse colon (PMID: 24151532) is observed. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252935).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)

      Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Eph receptor B2 with ab252935 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on human bladder cancer (PMID: 24151532, 25148033) is observed. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252935).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)
      Immunohistochemistry (Frozen sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling Eph receptor B2 with ab252935 at 1/50 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. No staining in mouse liver. Negative control: liver (PMID: 19366806). Counterstained with DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252935).

    • Immunohistochemistry (Frozen sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)
      Immunohistochemistry (Frozen sections) - Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver tissue labeling Eph receptor B2 with ab252935 at 1/50 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. No staining in rat liver. Negative control: liver (PMID: 19366806). Counterstained with DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252935).

    • Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)
      Anti-Eph receptor B2 antibody [EPR22427-268] - BSA and Azide free (ab255274)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab255274? Please let us know so that we can cite the reference in this datasheet.

    ab255274 has not yet been referenced specifically in any publications.

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