Anti-Eph receptor B4/HTK antibody [RM0056-6A14] (ab73259)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B4/HTK antibody [RM0056-6A14] (ab73259)](https://www.abcam.com/ps/products/73/ab73259/Images/ab73259-88402-anti-eph-receptor-b4-antibody-rm0056-6a14-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B4/HTK antibody [RM0056-6A14] (ab73259)")
Key features and details
- Rat monoclonal [RM0056-6A14] to Eph receptor B4/HTK
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-Eph receptor B4/HTK antibody [RM0056-6A14]
See all Eph receptor B4/HTK primary antibodies -
Description
Rat monoclonal [RM0056-6A14] to Eph receptor B4/HTK -
Host species
Rat -
Specificity
ab73259 is specific for mouse Eph receptor B4/HTK. It does not cross-react with recombinant mouse EphA2, EphA3, EphA4, EphA6, EphA7 and EphB6
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Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment corresponding to Mouse Eph receptor B4/HTK (extracellular).
Database link: P54761 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab73259 was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with recombinant mouse Eph receptor B4/HTK extracellular domain). -
Clonality
Monoclonal -
Clone number
RM0056-6A14 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
1/100 - 1/200.
Perform enzymatic antigen retrieval with 20 ug/ml proteinase K before commencing with IHC protocol. |
| Notes |
|---|
|
IHC-P
1/100 - 1/200. Perform enzymatic antigen retrieval with 20 ug/ml proteinase K before commencing with IHC protocol. |
Target
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Function
Receptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development. -
Tissue specificity
Abundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 13846 Mouse
- SwissProt: P54761 Mouse
- Unigene: 34533 Mouse
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Alternative names
- Ephb4 antibody
- EPHB4_HUMAN antibody
- Ephrin receptor EphB4 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B4/HTK antibody [RM0056-6A14] (ab73259)
ab73259 at 1/200 dilution, staining Eph receptor B4/HTK in LPS treated mouse kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B4/HTK antibody [RM0056-6A14] (ab73259)](https://www.abcam.com/ps/products/73/ab73259/Images/ab73259-88401-anti-eph-receptor-b4-antibody-rm0056-6a14-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B4/HTK antibody [RM0056-6A14] (ab73259)This image is courtesy of an anonymous Abreviewab73259 staining the Eph receptor B4/HTK in Mouse lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was not performed. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated Rabbit anti-rat polyclonal (1/200) was used as the secondary antibody.
Datasheets and documents
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Datasheet download
References (3)
ab73259 has been referenced in 3 publications.
- Sagar V et al. EPHB4 inhibition activates ER stress to promote immunogenic cell death of prostate cancer cells. Cell Death Dis 10:801 (2019). PubMed: 31641103
- Munro DAD et al. Refuting the hypothesis that semaphorin-3f/neuropilin-2 exclude blood vessels from the cap mesenchyme in the developing kidney. Dev Dyn 246:1047-1056 (2017). PubMed: 28929539
- Chen II et al. EphrinB2 regulates the emergence of a hemogenic endothelium from the aorta. Sci Rep 6:27195 (2016). ICC/IF ; Mouse . PubMed: 27250641
