Anti-ErbB2 / HER2 antibody [3B5] (ab16901)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [3B5] (ab16901)](https://www.abcam.com/ps/products/16/ab16901/Images/ab16901-273927-anti-erbb-2-antibody-3b5-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [3B5] (ab16901)")
Key features and details
- Mouse monoclonal [3B5] to ErbB2 / HER2
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-ErbB2 / HER2 antibody [3B5]
See all ErbB2 / HER2 primary antibodies -
Description
Mouse monoclonal [3B5] to ErbB2 / HER2 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog, Monkey
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Immunogen
Synthetic peptide corresponding to Human ErbB2/ HER2 aa 1242-1255 (C terminal).
Sequence:TAENPEYLGLDVPV
Database link: P04626 -
Epitope
Within amino acids 1242 - 1255 of human c-neu (TAENPEYLGLDVPV) -
Positive control
- SK-BR-3 cells or breast carcinoma tissue
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General notes
For paraffin sections, we recommend pretreating with a pressure cooker, but trypsin and heat will also work well. Including 0.05% saponin during staining of paraffin sections may help reduce background. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribriform growth type were stained by this antibody.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3B5 -
Isotype
IgG1 -
Research areas
Associated products
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Affibody® Molecule
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (3) |
Use at an assay dependent concentration.
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| WB | (5) |
Use a concentration of 2.5 µg/ml. Detects a band of approximately 190 kDa (predicted molecular weight: 138 kDa).
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| Flow Cyt | (1) |
1/50 - 1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. |
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WB
Use a concentration of 2.5 µg/ml. Detects a band of approximately 190 kDa (predicted molecular weight: 138 kDa). |
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Flow Cyt
1/50 - 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. -
Tissue specificity
Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth. -
Involvement in disease
Hereditary diffuse gastric cancer
Glioma
Ovarian cancer
Lung cancer
Gastric cancer
Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172). -
Cellular localization
Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. - Information by UniProt
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Database links
- Entrez Gene: 403883 Dog
- Entrez Gene: 2064 Human
- Entrez Gene: 13866 Mouse
- Entrez Gene: 24337 Rat
- Omim: 164870 Human
- SwissProt: O18735 Dog
- SwissProt: P04626 Human
- SwissProt: P70424 Mouse
see all -
Alternative names
- Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
- C erb B2/neu protein antibody
- CD340 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [3B5] (ab16901)This image is courtesy of an anonymous abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling ErbB2 / HER2 with ab16901 at 1/400 dilution. the tissue was fixed with formaldehyde; heat mediated antigen retrieval was performed using a acetate buffer pH6. An undiluted goat anti-mouse HRP conjugated seconday antibody was used.
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![Western blot - Anti-ErbB2 / HER2 antibody [3B5] (ab16901)](https://www.abcam.com/ps/products/16/ab16901/Images/ab16901-273933-anti-erbb-2-antibody-3b5-western-blot.png)
Western blot - Anti-ErbB2 / HER2 antibody [3B5] (ab16901)This image is courtesy of an anonymous abreview.Anti-ErbB2 / HER2 antibody [3B5] (ab16901) at 1/250 dilution + Human Huh7 whole cell lysate at 20 µg
Secondary
Goat anti-mouse HRP conjugate at 1/5000 dilution
Predicted band size: 138 kDa
Observed band size: 138 kDa
Additional bands at: 48 kDa (possible non-specific binding)
Exposure time: 5 minutesBlocking: 5% milk for 1 hour at 25°C.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [3B5] (ab16901)](https://www.abcam.com/ps/products/16/ab16901/Images/ab16901-25785-anti-erbb-2-antibody-3b5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [3B5] (ab16901)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Mamma tumor Her2Neu tissue labeling ErbB 2 with ab16901.
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![Western blot - Anti-ErbB2 / HER2 antibody [3B5] (ab16901)](https://www.abcam.com/ps/products/16/ab16901/Images/ab16901-25788-anti-erbb-2-antibody-3b5-western-blot.jpg)
Western blot - Anti-ErbB2 / HER2 antibody [3B5] (ab16901)Anti-ErbB2 / HER2 antibody [3B5] (ab16901) at 1 µg/ml + Whole cell lysate HEK293
Predicted band size: 138 kDa -
![Flow Cytometry - Anti-ErbB2 / HER2 antibody [3B5] (ab16901)](https://www.abcam.com/ps/products/16/ab16901/Images/ab16901-25787-anti-erbb-2-antibody-3b5-flow-cytometry.jpg)
Flow Cytometry - Anti-ErbB2 / HER2 antibody [3B5] (ab16901)Overlay histogram showing LoVo cells stained with ab16901 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16901, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in LoVo cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [3B5] (ab16901)](https://www.abcam.com/ps/products/16/ab16901/Images/ab16901-25786-anti-erbb2-antibody-3b5-cytoskeleton-marker-immunohistochemistry-breast-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [3B5] (ab16901)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma labeling ErbB 2 with ab16901 at 1/100.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [3B5] (ab16901)](https://www.abcam.com/ps/products/16/ab16901/Images/ab16901-396060-anti-erbb2-antibody-3b5-cytoskeleton-marker-immunohistochemistry-breast-cancer-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [3B5] (ab16901)Immunohistochemistry analysis of formalin fixed, paraffin embedded tissue section of human breast carcinoma labeling ErbB 2 with at16901 at dilution of 1/1000.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (63)
ab16901 has been referenced in 63 publications.
- Lim M et al. Landscape of Epidermal Growth Factor Receptor Heterodimers in Brain Metastases. Cancers (Basel) 14:N/A (2022). PubMed: 35158800
- Han D et al. The NF-κB/miR-488/ERBB2 axis modulates pancreatic cancer cell malignancy and tumor growth through cell cycle signaling. Cancer Biol Ther 23:294-309 (2022). PubMed: 35343383
- Sasamori H et al. Mutation Profiles of Ovarian Seromucinous Borderline Tumors in Japanese Patients. Curr Oncol 29:3658-3667 (2022). PubMed: 35621684
- Yücel D et al. Stimulation of Cardiomyocyte Proliferation Is Dependent on Species and Level of Maturation. Front Cell Dev Biol 10:806564 (2022). PubMed: 35663393
- Padmanabhan R et al. Quantification of the growth suppression of HER2+ breast cancer colonies under the effect of trastuzumab and PD-1/PD-L1 inhibitor. Front Oncol 12:977664 (2022). PubMed: 36568154