Recombinant Anti-ErbB2 / HER2 antibody [CAL27] (ab237715)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CAL27] (ab237715)](https://www.abcam.com/ps/products/237/ab237715/Images/ab237715-349357-anti-erbb-2-antibody-cal27-immunocytochemistry-breastcarcinoma-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CAL27] (ab237715)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [CAL27] to ErbB2 / HER2
- Suitable for: WB, ICC/IF, IP, Flow Cyt (Intra), IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ErbB2 / HER2 antibody [CAL27]
See all ErbB2 / HER2 primary antibodies -
Description
Rabbit monoclonal [CAL27] to ErbB2 / HER2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, Flow Cyt (Intra), IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, 4T1 and C6 whole cell lysates. IHC-P: Human breast carcinoma tissue. ICC/IF: SK-BR-3 cells. Flow Cyt (intra): SK-BR-3 cells. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purity >99%. -
Clonality
Monoclonal -
Clone number
CAL27 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 137 kDa).
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| ICC/IF |
1/500.
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| IP |
1/13.
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| Flow Cyt (Intra) |
1/500.
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| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 137 kDa). |
|
ICC/IF
1/500. |
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IP
1/13. |
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Flow Cyt (Intra)
1/500. |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. -
Tissue specificity
Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth. -
Involvement in disease
Hereditary diffuse gastric cancer
Glioma
Ovarian cancer
Lung cancer
Gastric cancer
Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172). -
Cellular localization
Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. - Information by UniProt
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Database links
- Entrez Gene: 2064 Human
- Entrez Gene: 13866 Mouse
- Entrez Gene: 24337 Rat
- Omim: 164870 Human
- SwissProt: P04626 Human
- SwissProt: P70424 Mouse
- SwissProt: P06494 Rat
- Unigene: 446352 Human
see all -
Alternative names
- Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
- C erb B2/neu protein antibody
- CD340 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CAL27] (ab237715)
Immunohistochemical analysis of human breast carcinoma tissue labeling ErbB2 / HER2 with ab237715 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the human breast carcinoma is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab237715 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [CAL27] (ab237715)](https://www.abcam.com/ps/products/237/ab237715/Images/ab237715-349358-anti-erbb-2-antibody-cal27-immunocytochemistry-immunofluorescence-skbr3-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [CAL27] (ab237715)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilised SK-BR-3 (human mammary gland adenocarcinoma cell line) cells labeling ERbB 2 with ab237715 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in SK-BR-3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Immunoprecipitation - Anti-ErbB2 / HER2 antibody [CAL27] (ab237715)](https://www.abcam.com/ps/products/237/ab237715/Images/ab237715-349359-anti-erbb-2-antibody-cal27-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-ErbB2 / HER2 antibody [CAL27] (ab237715)ERbB 2 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab237715 at 130 dilution. Western blot was performed from the immunoprecipitate using ab237715 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10 μg (Input).
Lane 2: ab237715 IP in HeLa whole lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237715 in HeLa whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds. -
![Flow Cytometry (Intracellular) - Anti-ErbB2 / HER2 antibody [CAL27] (ab237715)](https://www.abcam.com/ps/products/237/ab237715/Images/ab237715-349356-anti-erbb-2-antibody-cal27-flow-cytometry-skbr3-human.jpg)
Flow Cytometry (Intracellular) - Anti-ErbB2 / HER2 antibody [CAL27] (ab237715)Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methonao-permeabilized SH-BR-3 (human mammary gland adenocarcinoma cell line) cells labeling ErbB2 / HER2 with ab237715 at 1/500 dilution (red) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
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![Western blot - Anti-ErbB2 / HER2 antibody [CAL27] (ab237715)](https://www.abcam.com/ps/products/237/ab237715/Images/ab237715-349360-anti-erbb-2-antibody-cal27-western-blot-hela-human.jpg)
Western blot - Anti-ErbB2 / HER2 antibody [CAL27] (ab237715)Anti-ErbB2 / HER2 antibody [CAL27] (ab237715) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 137 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
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![Western blot - Anti-ErbB2 / HER2 antibody [CAL27] (ab237715)](https://www.abcam.com/ps/products/237/ab237715/Images/ab237715-349361-anti-erbb-2-antibody-cal27-western-blot-mouse-rat.jpg)
Western blot - Anti-ErbB2 / HER2 antibody [CAL27] (ab237715)All lanes : Anti-ErbB2 / HER2 antibody [CAL27] (ab237715) at 1/1000 dilution
Lane 1 : 4T1 (mouse mammary gland carcinoma cell line) whole cell lysate
Lane 2 : C6 (rat glial tumor glial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution
Predicted band size: 137 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CAL27] (ab237715)](https://www.abcam.com/ps/products/237/ab237715/Images/ab237715-315260-anti-erbb-2-antibody-cal27-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [CAL27] (ab237715)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for ErbB2 / HER2 using ab237715 at 0.3 μg/ml in immunohistochemical analysis.
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![Anti-ErbB2 / HER2 antibody [CAL27] (ab237715)](https://www.abcam.com/ps/products/237/ab237715/Images/ab237715-8-benefits-of-recombinant-antibodies.png)
Anti-ErbB2 / HER2 antibody [CAL27] (ab237715)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab237715 has been referenced in 3 publications.
- Song X et al. LncRNA-PAX8-AS1 Silencing Decreases Cell Viability, Enhances Apoptosis, and Suppresses Doxorubicin Resistance in Myeloid Leukemia via the miR-378g/ERBB2 Axis. Evid Based Complement Alternat Med 2022:2295044 (2022). PubMed: 36248434
- Liu J et al. Anthelminthic niclosamide inhibits tumor growth and invasion in cisplatin-resistant human epidermal growth factor receptor 2-positive breast cancer. Oncol Lett 22:666 (2021). PubMed: 34386088
- Yu W et al. The combination of circRNA-UMAD1 and Galectin-3 in peripheral circulation is a co-biomarker for predicting lymph node metastasis of thyroid carcinoma. Am J Transl Res 12:5399-5415 (2020). PubMed: 33042427