Recombinant Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)
![Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)](https://www.abcam.com/ps/products/53/ab53290/Images/ab53290-255420-anti-erbb-2-phospho-y1139-antibody-ep1046y-western-blot.jpg "Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1046Y] to ErbB2 / HER2 (phospho Y1139)
- Suitable for: WB, IP, Dot blot
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y]
See all ErbB2 / HER2 primary antibodies -
Description
Rabbit monoclonal [EP1046Y] to ErbB2 / HER2 (phospho Y1139) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Dot blotmore details
Unsuitable for: ICC/IF,IHC-Fr or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SKBR3 and A431 cell lysates treated with EGF.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1046Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 185 kDa (predicted molecular weight: 138 kDa).
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| IP |
1/30.
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| Dot blot |
1/1000.
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| Notes |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 185 kDa (predicted molecular weight: 138 kDa). |
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IP
1/30. |
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Dot blot
1/1000. |
Target
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Function
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. -
Tissue specificity
Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth. -
Involvement in disease
Hereditary diffuse gastric cancer
Glioma
Ovarian cancer
Lung cancer
Gastric cancer
Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172). -
Cellular localization
Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. - Information by UniProt
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Database links
- Entrez Gene: 2064 Human
- Omim: 164870 Human
- SwissProt: P04626 Human
- Unigene: 446352 Human
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Alternative names
- Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
- C erb B2/neu protein antibody
- CD340 antibody
see all
Images
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Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)All lanes : Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290) at 1/500 dilution
Lane 1 : A431 whole cell lysate - untreated
Lane 2 : A431 whole cell lysate - treated with Epidermal Growth Factor (EGF)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 138 kDa
Observed band size: 185 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)](https://www.abcam.com/ps/products/53/ab53290/Images/ab53290-272009-anti-erbb-2-phospho-y1139-antibody-ep1046y-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)ab53290 at 1/70 dilution immunoprecipitating ErbB 2 (phospho Y1139) in A431(human epidermoid carcinoma) whole cell lysate.
Lane 1 (input): A431treated with 100 ng/mL EGF for 10 minutes whole cell lysate 10μg
Lane 2 (+): ab53290 + A431 treated with 100 ng/mL EGF for 10 minutes whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab53290 in A431treated with 100 ng/mL EGF for 10 minutes whole cell lysate
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Blocking and Diluting buffer and concentration: 5% NFDM/TBST.
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![Dot Blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)](https://www.abcam.com/ps/products/53/ab53290/Images/ab53290-255419-anti-erbb-2-phospho-y1139-antibody-ep1046y-dot-blot.jpg)
Dot Blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)Dot blot analysis of ErbB 2 (pY1139) peptide (Lane 1) and ErbB 2 non-phospho peptide (Lane 2) labelling ErbB 2 (phospho Y1139) with ab53290 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)](https://www.abcam.com/ps/products/53/ab53290/Images/ab53290-66080-anti-erbb-2-phospho-y1139-antibody-ep1046y-western-blot.jpg)
Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)All lanes : Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290) at 1/1000 dilution
Lane 1 : SKBR3 cell lysate
Lane 2 : SKBR3 cell lysate - treated with EGF
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 138 kDa
Observed band size: 185 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)](https://www.abcam.com/ps/products/53/ab53290/Images/ab53290-66079-anti-erbb-2-phospho-y1139-antibody-ep1046y-western-blot.jpg)
Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)This image is courtesy of an Abreview by Surekha Pimple.All lanes : Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290) at 1/2000 dilution
Lane 1 : HTB-2O Human breast ductal carcinoma lysate untreated
Lanes 2-9 : HTB-2O Human breast ductal carcinoma lysate treated with HER-2 inhibitor
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated Mouse anti-Rabbit monoclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 138 kDa
Exposure time: 5 seconds
Lanes 2-9 are treated with an increasing concentration of HER2 inhibitor.
Blocking performed with 5% Milk for 1 hour at room temperature.
Antibody diluted in CPPT and incubated for 16 hours at 4°C.
Performed under denaturing conditions. -
![Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)](https://www.abcam.com/ps/products/53/ab53290/Images/ab53290-6-benefits-of-recombinant-antibodies.png)
Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab53290 has been referenced in 14 publications.
- Padmanabhan R et al. Quantification of the growth suppression of HER2+ breast cancer colonies under the effect of trastuzumab and PD-1/PD-L1 inhibitor. Front Oncol 12:977664 (2022). PubMed: 36568154
- Pereira PMR et al. Immuno-PET Detects Changes in Multi-RTK Tumor Cell Expression Levels in Response to Targeted Kinase Inhibition. J Nucl Med 62:366-371 (2021). PubMed: 32646879
- Yoshizawa R et al. Biphasic spatiotemporal regulation of GRB2 dynamics by p52SHC for transient RAS activation. Biophys Physicobiol 18:1-12 (2021). PubMed: 33665062
- Yoshizawa R et al. p52Shc regulates the sustainability of ERK activation in a RAF-independent manner. Mol Biol Cell 32:1838-1848 (2021). PubMed: 34260260
- Li J et al. Circular HER2 RNA positive triple negative breast cancer is sensitive to Pertuzumab. Mol Cancer 19:142 (2020). PubMed: 32917240