Recombinant Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)](https://www.abcam.com/ps/products/238/ab238437/Images/ab238437-332151-anti-erbb-2-phospho-y877-antibody-ep2324y-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2324Y] to ErbB2 / HER2 (phospho Y877) - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, Dot blot
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free
See all ErbB2 / HER2 primary antibodies -
Description
Rabbit monoclonal [EP2324Y] to ErbB2 / HER2 (phospho Y877) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, Dot blotmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human breast ductal infiltrating carcinoma tissue. ICC/IF: SK-BR-3 cells. Flow Cyt (intra): A431 cells (+/- EGF treatment). Dot Blot: ErbB2 / HER2 (pY877) peptide.
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General notes
ab238437 is the carrier-free version of ab108371.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2324Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238437 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 138 kDa.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC/IF |
Use at an assay dependent concentration.
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| Dot blot |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 138 kDa. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ICC/IF
Use at an assay dependent concentration. |
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Dot blot
Use at an assay dependent concentration. |
Target
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Function
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. -
Tissue specificity
Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth. -
Involvement in disease
Hereditary diffuse gastric cancer
Glioma
Ovarian cancer
Lung cancer
Gastric cancer
Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172). -
Cellular localization
Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. - Information by UniProt
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Database links
- Entrez Gene: 2064 Human
- Omim: 164870 Human
- SwissProt: P04626 Human
- Unigene: 446352 Human
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Alternative names
- Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
- C erb B2/neu protein antibody
- CD340 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)
ab108371, at 1/250, staining Human ErbB2 / HER2 (phospho Y877) in human breast ductal infiltrating carcinoma tissue by immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab108371)
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)](https://www.abcam.com/ps/products/238/ab238437/Images/ab238437-332153-anti-erbb-2-phospho-y877-antibody-ep2324y-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) untreated/treated with 200 ng/ml EGF for 15 minutes labeling ErbB 2 with purified ab108371 at 1/20.
Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab108371)
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![Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)](https://www.abcam.com/ps/products/238/ab238437/Images/ab238437-332150-anti-erbb-2-phospho-y877-antibody-ep2324y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437) -
![Dot Blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)](https://www.abcam.com/ps/products/238/ab238437/Images/ab238437-332152-anti-erbb-2-phospho-y877-antibody-ep2324y-dot-blot.jpg)
Dot Blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)Dot blot analysis of ErbB2 / HER2 (pY877) peptide (Lane 1) and ErbB2 non-phospho peptide (Lane 2) labeling ErbB 2 (phospho Y877) with ab108371 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab108371)
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![Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)](https://www.abcam.com/ps/products/238/ab238437/Images/ab238437-9-benefits-of-recombinant-antibodies.png)
Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab238437 has not yet been referenced specifically in any publications.
